Somatostatin receptor staining in FFPE sections using a ligand derivative dye as an alternative to immunostaining.
The confirmation of target expression in tissues is a prerequisite for molecular-targeted therapy. However, difficulties are sometimes associated with the production of appropriate antibodies against receptors. We herein developed a ligand derivative dye for the staining of receptors. The somatostat...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5300255?pdf=render |
| _version_ | 1818450633150169088 |
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| author | Koki Hasegawa Shinji Kudoh Takaaki Ito |
| author_facet | Koki Hasegawa Shinji Kudoh Takaaki Ito |
| author_sort | Koki Hasegawa |
| collection | DOAJ |
| description | The confirmation of target expression in tissues is a prerequisite for molecular-targeted therapy. However, difficulties are sometimes associated with the production of appropriate antibodies against receptors. We herein developed a ligand derivative dye for the staining of receptors. The somatostatin receptor (sstr) was selected as the target and FITC-octreotate as the detective agent. We performed a blot analysis to detect sstr in the transfer membrane. The sstr2 recombinant protein or cell lysate from a small cell lung carcinoma cell line (H69) was boiled and loaded onto SDS-PAGE, and the proteins were transferred to a membrane. Even after denaturing processes, FITC-octreotate still bound sstr on the membrane. Furthermore, FITC-octreotate depicted the expression of sstr in formalin-fixed and paraffin-embedded (FFPE) sections, a method that we named ligand derivative staining (LDS). The accuracies of immunostaining and LDS were compared at the points of the detection of sstr using FFPE sections of 30 neuroendocrine tumor specimens. The sensitivity of LDS was 81.8%, while those of immunostaining using anti-sstr2 and sstr5 antibodies were 72.7% and 63.6%, respectively. Thus, LDS appears to be superior to immunostaining. A ligand derivative may be used as a substitute for antibodies, and has the potential to support economical, simple, and accurate detection methods. |
| first_indexed | 2024-12-14T20:54:24Z |
| format | Article |
| id | doaj.art-c584b28eb499479785b7bec55dd6fbdd |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T20:54:24Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c584b28eb499479785b7bec55dd6fbdd2022-12-21T22:47:43ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01122e017203010.1371/journal.pone.0172030Somatostatin receptor staining in FFPE sections using a ligand derivative dye as an alternative to immunostaining.Koki HasegawaShinji KudohTakaaki ItoThe confirmation of target expression in tissues is a prerequisite for molecular-targeted therapy. However, difficulties are sometimes associated with the production of appropriate antibodies against receptors. We herein developed a ligand derivative dye for the staining of receptors. The somatostatin receptor (sstr) was selected as the target and FITC-octreotate as the detective agent. We performed a blot analysis to detect sstr in the transfer membrane. The sstr2 recombinant protein or cell lysate from a small cell lung carcinoma cell line (H69) was boiled and loaded onto SDS-PAGE, and the proteins were transferred to a membrane. Even after denaturing processes, FITC-octreotate still bound sstr on the membrane. Furthermore, FITC-octreotate depicted the expression of sstr in formalin-fixed and paraffin-embedded (FFPE) sections, a method that we named ligand derivative staining (LDS). The accuracies of immunostaining and LDS were compared at the points of the detection of sstr using FFPE sections of 30 neuroendocrine tumor specimens. The sensitivity of LDS was 81.8%, while those of immunostaining using anti-sstr2 and sstr5 antibodies were 72.7% and 63.6%, respectively. Thus, LDS appears to be superior to immunostaining. A ligand derivative may be used as a substitute for antibodies, and has the potential to support economical, simple, and accurate detection methods.http://europepmc.org/articles/PMC5300255?pdf=render |
| spellingShingle | Koki Hasegawa Shinji Kudoh Takaaki Ito Somatostatin receptor staining in FFPE sections using a ligand derivative dye as an alternative to immunostaining. PLoS ONE |
| title | Somatostatin receptor staining in FFPE sections using a ligand derivative dye as an alternative to immunostaining. |
| title_full | Somatostatin receptor staining in FFPE sections using a ligand derivative dye as an alternative to immunostaining. |
| title_fullStr | Somatostatin receptor staining in FFPE sections using a ligand derivative dye as an alternative to immunostaining. |
| title_full_unstemmed | Somatostatin receptor staining in FFPE sections using a ligand derivative dye as an alternative to immunostaining. |
| title_short | Somatostatin receptor staining in FFPE sections using a ligand derivative dye as an alternative to immunostaining. |
| title_sort | somatostatin receptor staining in ffpe sections using a ligand derivative dye as an alternative to immunostaining |
| url | http://europepmc.org/articles/PMC5300255?pdf=render |
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