<i>DREB1</i> and <i>DREB2</i> Genes in Garlic (<i>Allium sativum</i> L.): Genome-Wide Identification, Characterization, and Stress Response
Dehydration-responsive element-binding (DREB) transcription factors (TFs) of the A1 and A2 subfamilies involved in plant stress responses have not yet been reported in <i>Allium</i> species. In this study, we used bioinformatics and comparative transcriptomics to identify and characteriz...
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MDPI AG
2023-07-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/13/2538 |
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| author | Mikhail A. Filyushin Olga K. Anisimova Anna V. Shchennikova Elena Z. Kochieva |
| author_facet | Mikhail A. Filyushin Olga K. Anisimova Anna V. Shchennikova Elena Z. Kochieva |
| author_sort | Mikhail A. Filyushin |
| collection | DOAJ |
| description | Dehydration-responsive element-binding (DREB) transcription factors (TFs) of the A1 and A2 subfamilies involved in plant stress responses have not yet been reported in <i>Allium</i> species. In this study, we used bioinformatics and comparative transcriptomics to identify and characterize <i>DREB</i> A1 and A2 genes redundant in garlic (<i>Allium sativum</i> L.) and analyze their expression in <i>A. sativum</i> cultivars differing in the sensitivity to cold and <i>Fusarium</i> infection. Eight A1 (<i>AsaDREB1.1–1.8</i>) and eight A2 (<i>AsaDREB2.1–2.8</i>) genes were identified. <i>AsaDREB1.1–1.8</i> genes located in tandem on chromosome 1 had similar expression patterns, suggesting functional redundancy. <i>AsaDREB2.1–2.8</i> were scattered on different chromosomes and had organ- and genotype-specific expressions. <i>AsaDREB1</i> and <i>AsaDREB2</i> promoters contained 7 and 9 hormone- and stress-responsive <i>cis</i>-regulatory elements, respectively, and 13 sites associated with TF binding and plant development. In both <i>Fusarium</i>-resistant and -sensitive cultivars, fungal infection upregulated the <i>AsaDREB1.1–1.5</i>, <i>1.8</i>, <i>2.2</i>, <i>2.6</i>, and <i>2.8</i> genes and downregulated <i>AsaDREB2.5</i>, but the magnitude of response depended on the infection susceptibility of the cultivar. Cold exposure strongly upregulated the <i>AsaDREB1</i> genes, but downregulated most <i>AsaDREB2</i> genes. Our results provide the foundation for further functional analysis of the DREB TFs in <i>Allium</i> crops and could contribute to the breeding of stress-tolerant varieties. |
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| language | English |
| last_indexed | 2024-03-11T01:31:40Z |
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| spelling | doaj.art-c58513257ea64a45bb53e0cd242c7bb02023-11-18T17:18:55ZengMDPI AGPlants2223-77472023-07-011213253810.3390/plants12132538<i>DREB1</i> and <i>DREB2</i> Genes in Garlic (<i>Allium sativum</i> L.): Genome-Wide Identification, Characterization, and Stress ResponseMikhail A. Filyushin0Olga K. Anisimova1Anna V. Shchennikova2Elena Z. Kochieva3Research Center of Biotechnology, Institute of Bioengineering, Russian Academy of Sciences, Leninsky Ave. 33, Bld. 2, Moscow 119071, RussiaResearch Center of Biotechnology, Institute of Bioengineering, Russian Academy of Sciences, Leninsky Ave. 33, Bld. 2, Moscow 119071, RussiaResearch Center of Biotechnology, Institute of Bioengineering, Russian Academy of Sciences, Leninsky Ave. 33, Bld. 2, Moscow 119071, RussiaResearch Center of Biotechnology, Institute of Bioengineering, Russian Academy of Sciences, Leninsky Ave. 33, Bld. 2, Moscow 119071, RussiaDehydration-responsive element-binding (DREB) transcription factors (TFs) of the A1 and A2 subfamilies involved in plant stress responses have not yet been reported in <i>Allium</i> species. In this study, we used bioinformatics and comparative transcriptomics to identify and characterize <i>DREB</i> A1 and A2 genes redundant in garlic (<i>Allium sativum</i> L.) and analyze their expression in <i>A. sativum</i> cultivars differing in the sensitivity to cold and <i>Fusarium</i> infection. Eight A1 (<i>AsaDREB1.1–1.8</i>) and eight A2 (<i>AsaDREB2.1–2.8</i>) genes were identified. <i>AsaDREB1.1–1.8</i> genes located in tandem on chromosome 1 had similar expression patterns, suggesting functional redundancy. <i>AsaDREB2.1–2.8</i> were scattered on different chromosomes and had organ- and genotype-specific expressions. <i>AsaDREB1</i> and <i>AsaDREB2</i> promoters contained 7 and 9 hormone- and stress-responsive <i>cis</i>-regulatory elements, respectively, and 13 sites associated with TF binding and plant development. In both <i>Fusarium</i>-resistant and -sensitive cultivars, fungal infection upregulated the <i>AsaDREB1.1–1.5</i>, <i>1.8</i>, <i>2.2</i>, <i>2.6</i>, and <i>2.8</i> genes and downregulated <i>AsaDREB2.5</i>, but the magnitude of response depended on the infection susceptibility of the cultivar. Cold exposure strongly upregulated the <i>AsaDREB1</i> genes, but downregulated most <i>AsaDREB2</i> genes. Our results provide the foundation for further functional analysis of the DREB TFs in <i>Allium</i> crops and could contribute to the breeding of stress-tolerant varieties.https://www.mdpi.com/2223-7747/12/13/2538<i>Allium sativum</i> L.DREB proteinsgene structuregene expressioncold stress<i>Fusarium</i> |
| spellingShingle | Mikhail A. Filyushin Olga K. Anisimova Anna V. Shchennikova Elena Z. Kochieva <i>DREB1</i> and <i>DREB2</i> Genes in Garlic (<i>Allium sativum</i> L.): Genome-Wide Identification, Characterization, and Stress Response Plants <i>Allium sativum</i> L. DREB proteins gene structure gene expression cold stress <i>Fusarium</i> |
| title | <i>DREB1</i> and <i>DREB2</i> Genes in Garlic (<i>Allium sativum</i> L.): Genome-Wide Identification, Characterization, and Stress Response |
| title_full | <i>DREB1</i> and <i>DREB2</i> Genes in Garlic (<i>Allium sativum</i> L.): Genome-Wide Identification, Characterization, and Stress Response |
| title_fullStr | <i>DREB1</i> and <i>DREB2</i> Genes in Garlic (<i>Allium sativum</i> L.): Genome-Wide Identification, Characterization, and Stress Response |
| title_full_unstemmed | <i>DREB1</i> and <i>DREB2</i> Genes in Garlic (<i>Allium sativum</i> L.): Genome-Wide Identification, Characterization, and Stress Response |
| title_short | <i>DREB1</i> and <i>DREB2</i> Genes in Garlic (<i>Allium sativum</i> L.): Genome-Wide Identification, Characterization, and Stress Response |
| title_sort | i dreb1 i and i dreb2 i genes in garlic i allium sativum i l genome wide identification characterization and stress response |
| topic | <i>Allium sativum</i> L. DREB proteins gene structure gene expression cold stress <i>Fusarium</i> |
| url | https://www.mdpi.com/2223-7747/12/13/2538 |
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