Soybean Callus—A Potential Source of Tocopherols
In vitro cultures have been used as an effective means to achieve a high level of secondary metabolites in various plants, including soy. In this study, the contents of α-, γ-, and δ- tocopherol were quantified in soybean callus, and their amounts were compared to those of soybeans cultivated using...
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MDPI AG
2021-11-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/12/2571 |
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| author | Liliana Mureșan Doina Clapa Teodor Rusu Thomas T. Y. Wang Jae B. Park |
| author_facet | Liliana Mureșan Doina Clapa Teodor Rusu Thomas T. Y. Wang Jae B. Park |
| author_sort | Liliana Mureșan |
| collection | DOAJ |
| description | In vitro cultures have been used as an effective means to achieve a high level of secondary metabolites in various plants, including soy. In this study, the contents of α-, γ-, and δ- tocopherol were quantified in soybean callus, and their amounts were compared to those of soybeans cultivated using the conventional tillage system with three weed controls (respectively without herbicide and with two variants of herbicide). Soybean callus was produced using Murashige and Skoog 1962 (MS) medium supplemented with 0.1 mg/L 6-Benzylaminopurine (BAP) and 0. 1 mg/L Thidiazuron (TDZ). The highest amount of fresh callus was obtained from soybeans from the conventional tillage system with second weed control (S-metolachlor 960 g/L, imazamox 40 g/L, and propaquizafop 100 g/L) respectively 13,652.4 ± 1177.62 mg. The analyzed tocopherols were in much higher content in soy dry callus than the soybean seeds (5.63 µg/g compared with the 0.35 α-toco in soybean, 47.57 µg/g compared with 18.71 µg/g γ-toco or, 5.56 µg/g compared with 1.74 µg/g β-toco). The highest content of the three analyzed tocopherols was γ -tocopherol, both in callus and soybeans. Furthermore, the data showed that herbicides used in soybean culture significantly influenced both the in vitro callus production and the tocopherol callus content (<i>p</i> ˂ 0.05). Altogether, soybean callus can be an important source of tocopherols, and herbicides significantly influence in vitro callus production and the tocopherol callus content. |
| first_indexed | 2024-03-10T03:17:49Z |
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| language | English |
| last_indexed | 2024-03-10T03:17:49Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
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| series | Plants |
| spelling | doaj.art-c5879833c133442eb98e60858cb07f732023-11-23T10:10:06ZengMDPI AGPlants2223-77472021-11-011012257110.3390/plants10122571Soybean Callus—A Potential Source of TocopherolsLiliana Mureșan0Doina Clapa1Teodor Rusu2Thomas T. Y. Wang3Jae B. Park4Diet, Genomics and Immunology Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, USAInstitute of Advanced Horticulture Research of Transylvania, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Mănăștur 3-5, 400372 Cluj-Napoca, RomaniaFaculty of Agriculture, University of Agricultural Sciences and Veterinary Medicine, Mănăștur St. 3-5, 400372 Cluj-Napoca, RomaniaDiet, Genomics and Immunology Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, USADiet, Genomics and Immunology Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, USAIn vitro cultures have been used as an effective means to achieve a high level of secondary metabolites in various plants, including soy. In this study, the contents of α-, γ-, and δ- tocopherol were quantified in soybean callus, and their amounts were compared to those of soybeans cultivated using the conventional tillage system with three weed controls (respectively without herbicide and with two variants of herbicide). Soybean callus was produced using Murashige and Skoog 1962 (MS) medium supplemented with 0.1 mg/L 6-Benzylaminopurine (BAP) and 0. 1 mg/L Thidiazuron (TDZ). The highest amount of fresh callus was obtained from soybeans from the conventional tillage system with second weed control (S-metolachlor 960 g/L, imazamox 40 g/L, and propaquizafop 100 g/L) respectively 13,652.4 ± 1177.62 mg. The analyzed tocopherols were in much higher content in soy dry callus than the soybean seeds (5.63 µg/g compared with the 0.35 α-toco in soybean, 47.57 µg/g compared with 18.71 µg/g γ-toco or, 5.56 µg/g compared with 1.74 µg/g β-toco). The highest content of the three analyzed tocopherols was γ -tocopherol, both in callus and soybeans. Furthermore, the data showed that herbicides used in soybean culture significantly influenced both the in vitro callus production and the tocopherol callus content (<i>p</i> ˂ 0.05). Altogether, soybean callus can be an important source of tocopherols, and herbicides significantly influence in vitro callus production and the tocopherol callus content.https://www.mdpi.com/2223-7747/10/12/2571<i>Glycine max</i>in vitro cultureα tocopherolγ tocopherolδ tocopherolHPLC |
| spellingShingle | Liliana Mureșan Doina Clapa Teodor Rusu Thomas T. Y. Wang Jae B. Park Soybean Callus—A Potential Source of Tocopherols Plants <i>Glycine max</i> in vitro culture α tocopherol γ tocopherol δ tocopherol HPLC |
| title | Soybean Callus—A Potential Source of Tocopherols |
| title_full | Soybean Callus—A Potential Source of Tocopherols |
| title_fullStr | Soybean Callus—A Potential Source of Tocopherols |
| title_full_unstemmed | Soybean Callus—A Potential Source of Tocopherols |
| title_short | Soybean Callus—A Potential Source of Tocopherols |
| title_sort | soybean callus a potential source of tocopherols |
| topic | <i>Glycine max</i> in vitro culture α tocopherol γ tocopherol δ tocopherol HPLC |
| url | https://www.mdpi.com/2223-7747/10/12/2571 |
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