Study and Experimental Validation of the Functional Components and Mechanisms of <i>Hemerocallis citrina</i> Baroni in the Treatment of Lactation Deficiency
The function of <i>Hemerocallis citrina</i> Baroni (daylily) on promoting lactation is reported in several ancient Chinese medicine books. However, nowadays, there is no conclusive data to support this statement. In this study, we investigated the effect of <i>Hemerocallis citrina&...
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MDPI AG
2021-08-01
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| author | Jing Zhong Yuxuan Liang Yongchun Chen Jiawei Zhang Xiaoying Zou Jie Deng Da Wang Yuanming Sun Meiying Li |
| author_facet | Jing Zhong Yuxuan Liang Yongchun Chen Jiawei Zhang Xiaoying Zou Jie Deng Da Wang Yuanming Sun Meiying Li |
| author_sort | Jing Zhong |
| collection | DOAJ |
| description | The function of <i>Hemerocallis citrina</i> Baroni (daylily) on promoting lactation is reported in several ancient Chinese medicine books. However, nowadays, there is no conclusive data to support this statement. In this study, we investigated the effect of <i>Hemerocallis citrina</i> Baroni extract (HCE) on lactation insufficiency in chronic unpredictable mild stress (CUMS) dams and further explored the mechanism and functional components through network pharmacology. The results showed that HCE could increase the offspring’s weight, serum prolactin (PRL), and oxytocin (OT) level of CUMS dams. Network pharmacology analysis revealed that the facilitation of HCE on lactation is the result of the comprehensive action of 62 components on 209 targets and 260 pathways, among this network, quercetin, kaempferol, thymidine, etc., were the vital material basis, signal transducer and activator of transcription 3 (STAT3), mitogen activity protein kinase 1 (MAPK1), tumor protein P53 (TP53), etc., were the core targets, and the prolactin signaling pathway was the core pathway. In addition, verification test results showed that HCE regulated the abnormal expression of the prolactin signaling pathway, including STAT3, cyclin D1 (CCND1), MAPK1, MAPK8, nuclear factor NF-kappa-B p105 subunit (NFKB1), and tyrosine-protein kinase (JAK2). In conclusion, HCE exhibited a facilitation of lactation insufficiency, in which quercetin, kaempferol, thymidine, etc., were the most important material basis. The mechanism of this promotional effect is mediated by the prolactin signaling pathway in mammary gland. |
| first_indexed | 2024-03-10T08:49:04Z |
| format | Article |
| id | doaj.art-c58a10984a2542e08a133ba9155e0348 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T08:49:04Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-c58a10984a2542e08a133ba9155e03482023-11-22T07:39:55ZengMDPI AGFoods2304-81582021-08-01108186310.3390/foods10081863Study and Experimental Validation of the Functional Components and Mechanisms of <i>Hemerocallis citrina</i> Baroni in the Treatment of Lactation DeficiencyJing Zhong0Yuxuan Liang1Yongchun Chen2Jiawei Zhang3Xiaoying Zou4Jie Deng5Da Wang6Yuanming Sun7Meiying Li8College of Food Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, ChinaThe function of <i>Hemerocallis citrina</i> Baroni (daylily) on promoting lactation is reported in several ancient Chinese medicine books. However, nowadays, there is no conclusive data to support this statement. In this study, we investigated the effect of <i>Hemerocallis citrina</i> Baroni extract (HCE) on lactation insufficiency in chronic unpredictable mild stress (CUMS) dams and further explored the mechanism and functional components through network pharmacology. The results showed that HCE could increase the offspring’s weight, serum prolactin (PRL), and oxytocin (OT) level of CUMS dams. Network pharmacology analysis revealed that the facilitation of HCE on lactation is the result of the comprehensive action of 62 components on 209 targets and 260 pathways, among this network, quercetin, kaempferol, thymidine, etc., were the vital material basis, signal transducer and activator of transcription 3 (STAT3), mitogen activity protein kinase 1 (MAPK1), tumor protein P53 (TP53), etc., were the core targets, and the prolactin signaling pathway was the core pathway. In addition, verification test results showed that HCE regulated the abnormal expression of the prolactin signaling pathway, including STAT3, cyclin D1 (CCND1), MAPK1, MAPK8, nuclear factor NF-kappa-B p105 subunit (NFKB1), and tyrosine-protein kinase (JAK2). In conclusion, HCE exhibited a facilitation of lactation insufficiency, in which quercetin, kaempferol, thymidine, etc., were the most important material basis. The mechanism of this promotional effect is mediated by the prolactin signaling pathway in mammary gland.https://www.mdpi.com/2304-8158/10/8/1863<i>Hemerocallis citrina</i> Baronilactation insufficiencynetwork pharmacology |
| spellingShingle | Jing Zhong Yuxuan Liang Yongchun Chen Jiawei Zhang Xiaoying Zou Jie Deng Da Wang Yuanming Sun Meiying Li Study and Experimental Validation of the Functional Components and Mechanisms of <i>Hemerocallis citrina</i> Baroni in the Treatment of Lactation Deficiency Foods <i>Hemerocallis citrina</i> Baroni lactation insufficiency network pharmacology |
| title | Study and Experimental Validation of the Functional Components and Mechanisms of <i>Hemerocallis citrina</i> Baroni in the Treatment of Lactation Deficiency |
| title_full | Study and Experimental Validation of the Functional Components and Mechanisms of <i>Hemerocallis citrina</i> Baroni in the Treatment of Lactation Deficiency |
| title_fullStr | Study and Experimental Validation of the Functional Components and Mechanisms of <i>Hemerocallis citrina</i> Baroni in the Treatment of Lactation Deficiency |
| title_full_unstemmed | Study and Experimental Validation of the Functional Components and Mechanisms of <i>Hemerocallis citrina</i> Baroni in the Treatment of Lactation Deficiency |
| title_short | Study and Experimental Validation of the Functional Components and Mechanisms of <i>Hemerocallis citrina</i> Baroni in the Treatment of Lactation Deficiency |
| title_sort | study and experimental validation of the functional components and mechanisms of i hemerocallis citrina i baroni in the treatment of lactation deficiency |
| topic | <i>Hemerocallis citrina</i> Baroni lactation insufficiency network pharmacology |
| url | https://www.mdpi.com/2304-8158/10/8/1863 |
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