Ribosome Assembly as Antimicrobial Target
Many antibiotics target the ribosome and interfere with its translation cycle. Since translation is the source of all cellular proteins including ribosomal proteins, protein synthesis and ribosome assembly are interdependent. As a consequence, the activity of translation inhibitors might indirectly...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2016-05-01
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| Series: | Antibiotics |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2079-6382/5/2/18 |
| _version_ | 1818620901019615232 |
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| author | Rainer Nikolay Sabine Schmidt Renate Schlömer Elke Deuerling Knud H. Nierhaus |
| author_facet | Rainer Nikolay Sabine Schmidt Renate Schlömer Elke Deuerling Knud H. Nierhaus |
| author_sort | Rainer Nikolay |
| collection | DOAJ |
| description | Many antibiotics target the ribosome and interfere with its translation cycle. Since translation is the source of all cellular proteins including ribosomal proteins, protein synthesis and ribosome assembly are interdependent. As a consequence, the activity of translation inhibitors might indirectly cause defective ribosome assembly. Due to the difficulty in distinguishing between direct and indirect effects, and because assembly is probably a target in its own right, concepts are needed to identify small molecules that directly inhibit ribosome assembly. Here, we summarize the basic facts of ribosome targeting antibiotics. Furthermore, we present an in vivo screening strategy that focuses on ribosome assembly by a direct fluorescence based read-out that aims to identify and characterize small molecules acting as primary assembly inhibitors. |
| first_indexed | 2024-12-16T18:00:44Z |
| format | Article |
| id | doaj.art-c58cba4824374c9e9c491e015159831f |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-12-16T18:00:44Z |
| publishDate | 2016-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-c58cba4824374c9e9c491e015159831f2022-12-21T22:22:04ZengMDPI AGAntibiotics2079-63822016-05-01521810.3390/antibiotics5020018antibiotics5020018Ribosome Assembly as Antimicrobial TargetRainer Nikolay0Sabine Schmidt1Renate Schlömer2Elke Deuerling3Knud H. Nierhaus4Institut für Medizinische Physik und Biophysik, Charité—Universitätsmedizin Berlin, 10117 Berlin, GermanyMolecular Microbiology, University of Konstanz, Konstanz 78457, GermanyMolecular Microbiology, University of Konstanz, Konstanz 78457, GermanyMolecular Microbiology, University of Konstanz, Konstanz 78457, GermanyInstitut für Medizinische Physik und Biophysik, Charité—Universitätsmedizin Berlin, 10117 Berlin, GermanyMany antibiotics target the ribosome and interfere with its translation cycle. Since translation is the source of all cellular proteins including ribosomal proteins, protein synthesis and ribosome assembly are interdependent. As a consequence, the activity of translation inhibitors might indirectly cause defective ribosome assembly. Due to the difficulty in distinguishing between direct and indirect effects, and because assembly is probably a target in its own right, concepts are needed to identify small molecules that directly inhibit ribosome assembly. Here, we summarize the basic facts of ribosome targeting antibiotics. Furthermore, we present an in vivo screening strategy that focuses on ribosome assembly by a direct fluorescence based read-out that aims to identify and characterize small molecules acting as primary assembly inhibitors.http://www.mdpi.com/2079-6382/5/2/18protein synthesis as preferential target of antibioticsribosome as antibiotic targetinhibitors of ribosome assemblyconcepts for identifying assembly inhibitors |
| spellingShingle | Rainer Nikolay Sabine Schmidt Renate Schlömer Elke Deuerling Knud H. Nierhaus Ribosome Assembly as Antimicrobial Target Antibiotics protein synthesis as preferential target of antibiotics ribosome as antibiotic target inhibitors of ribosome assembly concepts for identifying assembly inhibitors |
| title | Ribosome Assembly as Antimicrobial Target |
| title_full | Ribosome Assembly as Antimicrobial Target |
| title_fullStr | Ribosome Assembly as Antimicrobial Target |
| title_full_unstemmed | Ribosome Assembly as Antimicrobial Target |
| title_short | Ribosome Assembly as Antimicrobial Target |
| title_sort | ribosome assembly as antimicrobial target |
| topic | protein synthesis as preferential target of antibiotics ribosome as antibiotic target inhibitors of ribosome assembly concepts for identifying assembly inhibitors |
| url | http://www.mdpi.com/2079-6382/5/2/18 |
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