Basement membrane proteins in extracellular matrix characterize NF1 neurofibroma development and response to MEK inhibitor
Neurofibromatosis type 1 (NF1) is one of the most common tumor-predisposing genetic disorders. Neurofibromas are NF1-associated benign tumors. A hallmark feature of neurofibromas is an abundant collagen-rich extracellular matrix (ECM) that constitutes more than 50% of the tumor dry weight. However,...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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American Society for Clinical Investigation
2023-06-01
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| Series: | The Journal of Clinical Investigation |
| Subjects: | |
| Online Access: | https://doi.org/10.1172/JCI168227 |
| _version_ | 1797634458868776960 |
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| author | Chunhui Jiang Ashwani Kumar Ze Yu Tracey Shipman Yong Wang Renee M. McKay Chao Xing Lu Q. Le |
| author_facet | Chunhui Jiang Ashwani Kumar Ze Yu Tracey Shipman Yong Wang Renee M. McKay Chao Xing Lu Q. Le |
| author_sort | Chunhui Jiang |
| collection | DOAJ |
| description | Neurofibromatosis type 1 (NF1) is one of the most common tumor-predisposing genetic disorders. Neurofibromas are NF1-associated benign tumors. A hallmark feature of neurofibromas is an abundant collagen-rich extracellular matrix (ECM) that constitutes more than 50% of the tumor dry weight. However, little is known about the mechanism underlying ECM deposition during neurofibroma development and treatment response. We performed a systematic investigation of ECM enrichment during plexiform neurofibroma (pNF) development and identified basement membrane (BM) proteins, rather than major collagen isoforms, as the most upregulated ECM component. Following MEK inhibitor treatment, the ECM profile displayed an overall downregulation signature, suggesting ECM reduction as a therapeutic benefit of MEK inhibition. Through these proteomic studies, TGF-β1 signaling was identified as playing a role in ECM dynamics. Indeed, TGF-β1 overexpression promoted pNF progression in vivo. Furthermore, by integrating single-cell RNA sequencing, we found that immune cells including macrophages and T cells produce TGF-β1 to induce Schwann cells to produce and deposit BM proteins for ECM remodeling. Following Nf1 loss, neoplastic Schwann cells further increased BM protein deposition in response to TGF-β1. Our data delineate the regulation governing ECM dynamics in pNF and suggest that BM proteins could serve as biomarkers for disease diagnosis and treatment response. |
| first_indexed | 2024-03-11T12:09:07Z |
| format | Article |
| id | doaj.art-c58d1cf601994e0eb228352447eddcec |
| institution | Directory Open Access Journal |
| issn | 1558-8238 |
| language | English |
| last_indexed | 2024-03-11T12:09:07Z |
| publishDate | 2023-06-01 |
| publisher | American Society for Clinical Investigation |
| record_format | Article |
| series | The Journal of Clinical Investigation |
| spelling | doaj.art-c58d1cf601994e0eb228352447eddcec2023-11-07T16:20:29ZengAmerican Society for Clinical InvestigationThe Journal of Clinical Investigation1558-82382023-06-0113312Basement membrane proteins in extracellular matrix characterize NF1 neurofibroma development and response to MEK inhibitorChunhui JiangAshwani KumarZe YuTracey ShipmanYong WangRenee M. McKayChao XingLu Q. LeNeurofibromatosis type 1 (NF1) is one of the most common tumor-predisposing genetic disorders. Neurofibromas are NF1-associated benign tumors. A hallmark feature of neurofibromas is an abundant collagen-rich extracellular matrix (ECM) that constitutes more than 50% of the tumor dry weight. However, little is known about the mechanism underlying ECM deposition during neurofibroma development and treatment response. We performed a systematic investigation of ECM enrichment during plexiform neurofibroma (pNF) development and identified basement membrane (BM) proteins, rather than major collagen isoforms, as the most upregulated ECM component. Following MEK inhibitor treatment, the ECM profile displayed an overall downregulation signature, suggesting ECM reduction as a therapeutic benefit of MEK inhibition. Through these proteomic studies, TGF-β1 signaling was identified as playing a role in ECM dynamics. Indeed, TGF-β1 overexpression promoted pNF progression in vivo. Furthermore, by integrating single-cell RNA sequencing, we found that immune cells including macrophages and T cells produce TGF-β1 to induce Schwann cells to produce and deposit BM proteins for ECM remodeling. Following Nf1 loss, neoplastic Schwann cells further increased BM protein deposition in response to TGF-β1. Our data delineate the regulation governing ECM dynamics in pNF and suggest that BM proteins could serve as biomarkers for disease diagnosis and treatment response.https://doi.org/10.1172/JCI168227Oncology |
| spellingShingle | Chunhui Jiang Ashwani Kumar Ze Yu Tracey Shipman Yong Wang Renee M. McKay Chao Xing Lu Q. Le Basement membrane proteins in extracellular matrix characterize NF1 neurofibroma development and response to MEK inhibitor The Journal of Clinical Investigation Oncology |
| title | Basement membrane proteins in extracellular matrix characterize NF1 neurofibroma development and response to MEK inhibitor |
| title_full | Basement membrane proteins in extracellular matrix characterize NF1 neurofibroma development and response to MEK inhibitor |
| title_fullStr | Basement membrane proteins in extracellular matrix characterize NF1 neurofibroma development and response to MEK inhibitor |
| title_full_unstemmed | Basement membrane proteins in extracellular matrix characterize NF1 neurofibroma development and response to MEK inhibitor |
| title_short | Basement membrane proteins in extracellular matrix characterize NF1 neurofibroma development and response to MEK inhibitor |
| title_sort | basement membrane proteins in extracellular matrix characterize nf1 neurofibroma development and response to mek inhibitor |
| topic | Oncology |
| url | https://doi.org/10.1172/JCI168227 |
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