Combined Transcriptome and Metabolome Analysis of a New Species of Microalgae from the Tibetan Plateau and Its Response to Sewage Treatment
Microalgae are pivotal in maintaining water quality in the lakes and rivers of Qinghai-Tibet plateau. The optimum sewage treatment conditions for <i>Desmodesmus</i> sp. Are, temperature: 20–25 °C, light intensity: 3000–8000 lx, and pH: 7.0–7.5, identified based on orthogonal experiments....
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MDPI AG
2022-10-01
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| author | Jinhu Wang Qiangying Zhang Junyu Chen Jinna Zhou Jing Li Yanli Wei Balmukunda Regmi Duo Bu |
| author_facet | Jinhu Wang Qiangying Zhang Junyu Chen Jinna Zhou Jing Li Yanli Wei Balmukunda Regmi Duo Bu |
| author_sort | Jinhu Wang |
| collection | DOAJ |
| description | Microalgae are pivotal in maintaining water quality in the lakes and rivers of Qinghai-Tibet plateau. The optimum sewage treatment conditions for <i>Desmodesmus</i> sp. Are, temperature: 20–25 °C, light intensity: 3000–8000 lx, and pH: 7.0–7.5, identified based on orthogonal experiments. The maximum removal rate of total nitrogen, total phosphorus, and chemical oxygen demand was more than 95% in the actual sewage treatment. The sewage treatment capacity of <i>Desmodesmus</i> sp. From plateau is higher than that from plains under the same treatment conditions. To identify the differentially expressed genes and metabolites in <i>Desmodesmus</i> sp. In response to sewage treatment, a combination of metabolomics and transcriptomics was employed to the microalgae with and without sewage treatment. The results showed that the oxidative phosphorylation, photosynthesis, and propanoate metabolism pathways were the most significantly enriched pathways in sewage treatment. Furthermore, the metabolism of adenosine diphosphate, 2-oxobutanoate, and succinate were significantly upregulated, downregulated, and both upregulated and downregulated, respectively, as shown by the combined transcriptome and metabolome analysis. Additionally, we found that sewage treatment could also induce numerous changes in the primary metabolism, such as carbohydrate, fatty acid biosynthesis, and amino acid metabolism when compared with control. Overall, our results should improve fundamental knowledge of molecular responses to <i>D</i><i>esmodesmus</i> sp. in sewage treatment and contribute to the design of strategies in microalgae response to sewage treatment. |
| first_indexed | 2024-03-09T18:33:33Z |
| format | Article |
| id | doaj.art-c59016e22d0146458bd31463b0e6c793 |
| institution | Directory Open Access Journal |
| issn | 2073-4441 |
| language | English |
| last_indexed | 2024-03-09T18:33:33Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Water |
| spelling | doaj.art-c59016e22d0146458bd31463b0e6c7932023-11-24T07:18:43ZengMDPI AGWater2073-44412022-10-011421339110.3390/w14213391Combined Transcriptome and Metabolome Analysis of a New Species of Microalgae from the Tibetan Plateau and Its Response to Sewage TreatmentJinhu Wang0Qiangying Zhang1Junyu Chen2Jinna Zhou3Jing Li4Yanli Wei5Balmukunda Regmi6Duo Bu7College of Science, Tibet University, 10 East Zangda Road, Lhasa 850000, ChinaCollege of Science, Tibet University, 10 East Zangda Road, Lhasa 850000, ChinaCollege of Science, Tibet University, 10 East Zangda Road, Lhasa 850000, ChinaCollege of Science, Tibet University, 10 East Zangda Road, Lhasa 850000, ChinaCollege of Science, Tibet University, 10 East Zangda Road, Lhasa 850000, ChinaCollege of Science, Tibet University, 10 East Zangda Road, Lhasa 850000, ChinaDepartment of Pharmacy, Institute of Medicine, Tribhuvan University, Maharajgunj, Kathmandu 44606, NepalCollege of Science, Tibet University, 10 East Zangda Road, Lhasa 850000, ChinaMicroalgae are pivotal in maintaining water quality in the lakes and rivers of Qinghai-Tibet plateau. The optimum sewage treatment conditions for <i>Desmodesmus</i> sp. Are, temperature: 20–25 °C, light intensity: 3000–8000 lx, and pH: 7.0–7.5, identified based on orthogonal experiments. The maximum removal rate of total nitrogen, total phosphorus, and chemical oxygen demand was more than 95% in the actual sewage treatment. The sewage treatment capacity of <i>Desmodesmus</i> sp. From plateau is higher than that from plains under the same treatment conditions. To identify the differentially expressed genes and metabolites in <i>Desmodesmus</i> sp. In response to sewage treatment, a combination of metabolomics and transcriptomics was employed to the microalgae with and without sewage treatment. The results showed that the oxidative phosphorylation, photosynthesis, and propanoate metabolism pathways were the most significantly enriched pathways in sewage treatment. Furthermore, the metabolism of adenosine diphosphate, 2-oxobutanoate, and succinate were significantly upregulated, downregulated, and both upregulated and downregulated, respectively, as shown by the combined transcriptome and metabolome analysis. Additionally, we found that sewage treatment could also induce numerous changes in the primary metabolism, such as carbohydrate, fatty acid biosynthesis, and amino acid metabolism when compared with control. Overall, our results should improve fundamental knowledge of molecular responses to <i>D</i><i>esmodesmus</i> sp. in sewage treatment and contribute to the design of strategies in microalgae response to sewage treatment.https://www.mdpi.com/2073-4441/14/21/3391Qinghai–Tibet plateau<i>Desmodesmus</i>sewage treatmenttranscriptomicsmetabolomicscorrelation analysis |
| spellingShingle | Jinhu Wang Qiangying Zhang Junyu Chen Jinna Zhou Jing Li Yanli Wei Balmukunda Regmi Duo Bu Combined Transcriptome and Metabolome Analysis of a New Species of Microalgae from the Tibetan Plateau and Its Response to Sewage Treatment Water Qinghai–Tibet plateau <i>Desmodesmus</i> sewage treatment transcriptomics metabolomics correlation analysis |
| title | Combined Transcriptome and Metabolome Analysis of a New Species of Microalgae from the Tibetan Plateau and Its Response to Sewage Treatment |
| title_full | Combined Transcriptome and Metabolome Analysis of a New Species of Microalgae from the Tibetan Plateau and Its Response to Sewage Treatment |
| title_fullStr | Combined Transcriptome and Metabolome Analysis of a New Species of Microalgae from the Tibetan Plateau and Its Response to Sewage Treatment |
| title_full_unstemmed | Combined Transcriptome and Metabolome Analysis of a New Species of Microalgae from the Tibetan Plateau and Its Response to Sewage Treatment |
| title_short | Combined Transcriptome and Metabolome Analysis of a New Species of Microalgae from the Tibetan Plateau and Its Response to Sewage Treatment |
| title_sort | combined transcriptome and metabolome analysis of a new species of microalgae from the tibetan plateau and its response to sewage treatment |
| topic | Qinghai–Tibet plateau <i>Desmodesmus</i> sewage treatment transcriptomics metabolomics correlation analysis |
| url | https://www.mdpi.com/2073-4441/14/21/3391 |
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