Quercetin Production and Phenylalanine Ammonia Lyase Activity Enhancement by Putrescine and Benzyl Amino Purine in Vitro Culture of Achillea Millefolium Linn
Introduction: Quercetin is observed in abundance in Achillea millefolium Linn and it has been employed for the treatment of diseases, infections, as well as health issues. This study examines the individual and combined impact of putrescine and benzyl amino purine (BAP) with the stimulation of querc...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2020-11-01
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| Series: | Journal of Fasting and Health |
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| Online Access: | https://jnfh.mums.ac.ir/article_16900_0dca8895ab800a2b6085a271e261dd19.pdf |
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| author | Solmaz Beldarvandi Askar Hossein Afshari Zahra Oraghi Ardebili |
| author_facet | Solmaz Beldarvandi Askar Hossein Afshari Zahra Oraghi Ardebili |
| author_sort | Solmaz Beldarvandi Askar |
| collection | DOAJ |
| description | Introduction: Quercetin is observed in abundance in Achillea millefolium Linn and it has been employed for the treatment of diseases, infections, as well as health issues. This study examines the individual and combined impact of putrescine and benzyl amino purine (BAP) with the stimulation of quercetin production and the activity of phenylalanine ammonia-lyase (PAL) in Achillea millefolium Linn cultures. Methods: Seedlings with four leaves were cut and transferred to MS basal medium (Murashige and Skoog Basal Medium). At the next stage, different concentrations of putrescine were applied. Quercetin was identified through using HPLC, and the assessment of PAL activity was completed by accounting for the level of cinnamic acid produced at 290 nm. Different experimental data were analyzed after collection using SAS software and Duncan’s test was employed to compare the means. Results: With the addition of putrescine, the quercetin production, BAP, and PAL activities were remarkably affected. Moreover, quercetin production and PAL activity were enhanced through stimulation applied using putrescine with varying concentrations. When various concentrations of putrescine were applied, the fact was illustrated that 2 mg/L and 1 mg/L experienced the highest amount of quercetin and PAL activity, respectively. Conclusion: This particular finding suggests that combining elicitors and plant growth regulators is a beneficial strategy adopted in enhancing PAL activity and the production of quercetin for in vitro cultures of A. millefolium. |
| first_indexed | 2024-12-16T15:30:19Z |
| format | Article |
| id | doaj.art-c596138c7238417ead699a972c1e1b2a |
| institution | Directory Open Access Journal |
| issn | 2345-2587 2345-2587 |
| language | English |
| last_indexed | 2024-12-16T15:30:19Z |
| publishDate | 2020-11-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Journal of Fasting and Health |
| spelling | doaj.art-c596138c7238417ead699a972c1e1b2a2022-12-21T22:26:22ZengMashhad University of Medical SciencesJournal of Fasting and Health2345-25872345-25872020-11-0184 (Special Issue on Food Safety)26427210.22038/jnfh.2020.49980.127516900Quercetin Production and Phenylalanine Ammonia Lyase Activity Enhancement by Putrescine and Benzyl Amino Purine in Vitro Culture of Achillea Millefolium LinnSolmaz Beldarvandi Askar0Hossein Afshari1Zahra Oraghi Ardebili2Department of Biotechnology, Damghan Branch, Islamic Azad University, Damghan, Iran.Department of Horticulture, Faculty of Agriculture,Damghan Branch, Islamic Azad University, Damghan, Iran.Department of Biology, Garmsar Branch, Islamic Azad University, Garmsar, Iran.Introduction: Quercetin is observed in abundance in Achillea millefolium Linn and it has been employed for the treatment of diseases, infections, as well as health issues. This study examines the individual and combined impact of putrescine and benzyl amino purine (BAP) with the stimulation of quercetin production and the activity of phenylalanine ammonia-lyase (PAL) in Achillea millefolium Linn cultures. Methods: Seedlings with four leaves were cut and transferred to MS basal medium (Murashige and Skoog Basal Medium). At the next stage, different concentrations of putrescine were applied. Quercetin was identified through using HPLC, and the assessment of PAL activity was completed by accounting for the level of cinnamic acid produced at 290 nm. Different experimental data were analyzed after collection using SAS software and Duncan’s test was employed to compare the means. Results: With the addition of putrescine, the quercetin production, BAP, and PAL activities were remarkably affected. Moreover, quercetin production and PAL activity were enhanced through stimulation applied using putrescine with varying concentrations. When various concentrations of putrescine were applied, the fact was illustrated that 2 mg/L and 1 mg/L experienced the highest amount of quercetin and PAL activity, respectively. Conclusion: This particular finding suggests that combining elicitors and plant growth regulators is a beneficial strategy adopted in enhancing PAL activity and the production of quercetin for in vitro cultures of A. millefolium.https://jnfh.mums.ac.ir/article_16900_0dca8895ab800a2b6085a271e261dd19.pdfachillea millefolium linnbenzyl amino purineputrescinequercetin |
| spellingShingle | Solmaz Beldarvandi Askar Hossein Afshari Zahra Oraghi Ardebili Quercetin Production and Phenylalanine Ammonia Lyase Activity Enhancement by Putrescine and Benzyl Amino Purine in Vitro Culture of Achillea Millefolium Linn Journal of Fasting and Health achillea millefolium linn benzyl amino purine putrescine quercetin |
| title | Quercetin Production and Phenylalanine Ammonia Lyase Activity Enhancement by Putrescine and Benzyl Amino Purine in Vitro Culture of Achillea Millefolium Linn |
| title_full | Quercetin Production and Phenylalanine Ammonia Lyase Activity Enhancement by Putrescine and Benzyl Amino Purine in Vitro Culture of Achillea Millefolium Linn |
| title_fullStr | Quercetin Production and Phenylalanine Ammonia Lyase Activity Enhancement by Putrescine and Benzyl Amino Purine in Vitro Culture of Achillea Millefolium Linn |
| title_full_unstemmed | Quercetin Production and Phenylalanine Ammonia Lyase Activity Enhancement by Putrescine and Benzyl Amino Purine in Vitro Culture of Achillea Millefolium Linn |
| title_short | Quercetin Production and Phenylalanine Ammonia Lyase Activity Enhancement by Putrescine and Benzyl Amino Purine in Vitro Culture of Achillea Millefolium Linn |
| title_sort | quercetin production and phenylalanine ammonia lyase activity enhancement by putrescine and benzyl amino purine in vitro culture of achillea millefolium linn |
| topic | achillea millefolium linn benzyl amino purine putrescine quercetin |
| url | https://jnfh.mums.ac.ir/article_16900_0dca8895ab800a2b6085a271e261dd19.pdf |
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