Valorization of the Antioxidant Effect of Mantua PGI Pear By-Product Extracts: Preparation, Analysis and Biological Investigation
For improving the management of the production chain of PGI Mantua pears (which comprises many varieties, including Abate Fetel), applying the cardinal principles of circular economy and sustainability, the fruits with diseases or defects were recovered for producing dried rounds of pears from the A...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/1/144 |
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| author | Carlotta Bollati Stefania Marzorati Lorenzo Cecchi Martina Bartolomei Jianqiang Li Maria Bellumori Lorenza d’Adduzio Luisella Verotta Laura Piazza Anna Arnoldi Nadia Mulinacci Carmen Lammi |
| author_facet | Carlotta Bollati Stefania Marzorati Lorenzo Cecchi Martina Bartolomei Jianqiang Li Maria Bellumori Lorenza d’Adduzio Luisella Verotta Laura Piazza Anna Arnoldi Nadia Mulinacci Carmen Lammi |
| author_sort | Carlotta Bollati |
| collection | DOAJ |
| description | For improving the management of the production chain of PGI Mantua pears (which comprises many varieties, including Abate Fetel), applying the cardinal principles of circular economy and sustainability, the fruits with diseases or defects were recovered for producing dried rounds of pears from the Abate Fetel cultivar, a new product with high nutritional value that extends the remaining life. This process led to the production of secondary and residual by-products, which are mainly composed of the highest and lowest part of the fruits, comprising seeds, pulps, peels and petioles. Hence, this study was focused on the valorization of these secondary by-products of Abate Fetel pears through the production of pear extracts using traditional and “green” extraction methods that involve the use of supercritical CO<sub>2</sub> fluid extraction. The produced extracts, together with a reference solvent-derived extract, were analyzed by HPLC-ESI-MS, and in parallel, their direct and cellular antioxidant activity were assessed. Evidence has indicated that all the tested extracts reduced the H<sub>2</sub>O<sub>2</sub>-induced reactive oxygen species (ROS), lipid peroxidation and nitric oxide (NO) levels, respectively, in human intestinal Caco-2 cells. Hence, this study clearly suggests that extracts obtained from Mantuan PGI pear by-products may be used as valuable sources of bioactive upcycled phytocomplex for the development of dietary supplements and/or functional foods. |
| first_indexed | 2024-03-09T13:46:32Z |
| format | Article |
| id | doaj.art-c597732187574a13ba170d7b11483ce8 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T13:46:32Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-c597732187574a13ba170d7b11483ce82023-11-30T20:58:56ZengMDPI AGAntioxidants2076-39212023-01-0112114410.3390/antiox12010144Valorization of the Antioxidant Effect of Mantua PGI Pear By-Product Extracts: Preparation, Analysis and Biological InvestigationCarlotta Bollati0Stefania Marzorati1Lorenzo Cecchi2Martina Bartolomei3Jianqiang Li4Maria Bellumori5Lorenza d’Adduzio6Luisella Verotta7Laura Piazza8Anna Arnoldi9Nadia Mulinacci10Carmen Lammi11Department of Pharmaceutical Sciences, University of Milan, 20133 Milan, ItalyDepartment of Environmental Science and Policy, Università degli Studi di Milano, 20133 Milan, ItalyPharmaceutical and Nutraceutical Section, Department of Neuroscience, Psychology, Drug and Child Health, University of Florence, 50019 Florence, ItalyDepartment of Pharmaceutical Sciences, University of Milan, 20133 Milan, ItalyDepartment of Pharmaceutical Sciences, University of Milan, 20133 Milan, ItalyPharmaceutical and Nutraceutical Section, Department of Neuroscience, Psychology, Drug and Child Health, University of Florence, 50019 Florence, ItalyDepartment of Pharmaceutical Sciences, University of Milan, 20133 Milan, ItalyDepartment of Environmental Science and Policy, Università degli Studi di Milano, 20133 Milan, ItalyDepartment of Environmental Science and Policy, Università degli Studi di Milano, 20133 Milan, ItalyDepartment of Pharmaceutical Sciences, University of Milan, 20133 Milan, ItalyPharmaceutical and Nutraceutical Section, Department of Neuroscience, Psychology, Drug and Child Health, University of Florence, 50019 Florence, ItalyDepartment of Pharmaceutical Sciences, University of Milan, 20133 Milan, ItalyFor improving the management of the production chain of PGI Mantua pears (which comprises many varieties, including Abate Fetel), applying the cardinal principles of circular economy and sustainability, the fruits with diseases or defects were recovered for producing dried rounds of pears from the Abate Fetel cultivar, a new product with high nutritional value that extends the remaining life. This process led to the production of secondary and residual by-products, which are mainly composed of the highest and lowest part of the fruits, comprising seeds, pulps, peels and petioles. Hence, this study was focused on the valorization of these secondary by-products of Abate Fetel pears through the production of pear extracts using traditional and “green” extraction methods that involve the use of supercritical CO<sub>2</sub> fluid extraction. The produced extracts, together with a reference solvent-derived extract, were analyzed by HPLC-ESI-MS, and in parallel, their direct and cellular antioxidant activity were assessed. Evidence has indicated that all the tested extracts reduced the H<sub>2</sub>O<sub>2</sub>-induced reactive oxygen species (ROS), lipid peroxidation and nitric oxide (NO) levels, respectively, in human intestinal Caco-2 cells. Hence, this study clearly suggests that extracts obtained from Mantuan PGI pear by-products may be used as valuable sources of bioactive upcycled phytocomplex for the development of dietary supplements and/or functional foods.https://www.mdpi.com/2076-3921/12/1/144antioxidant activitycircular economyMantua pearpear extractssupercritical CO<sub>2</sub> extraction |
| spellingShingle | Carlotta Bollati Stefania Marzorati Lorenzo Cecchi Martina Bartolomei Jianqiang Li Maria Bellumori Lorenza d’Adduzio Luisella Verotta Laura Piazza Anna Arnoldi Nadia Mulinacci Carmen Lammi Valorization of the Antioxidant Effect of Mantua PGI Pear By-Product Extracts: Preparation, Analysis and Biological Investigation Antioxidants antioxidant activity circular economy Mantua pear pear extracts supercritical CO<sub>2</sub> extraction |
| title | Valorization of the Antioxidant Effect of Mantua PGI Pear By-Product Extracts: Preparation, Analysis and Biological Investigation |
| title_full | Valorization of the Antioxidant Effect of Mantua PGI Pear By-Product Extracts: Preparation, Analysis and Biological Investigation |
| title_fullStr | Valorization of the Antioxidant Effect of Mantua PGI Pear By-Product Extracts: Preparation, Analysis and Biological Investigation |
| title_full_unstemmed | Valorization of the Antioxidant Effect of Mantua PGI Pear By-Product Extracts: Preparation, Analysis and Biological Investigation |
| title_short | Valorization of the Antioxidant Effect of Mantua PGI Pear By-Product Extracts: Preparation, Analysis and Biological Investigation |
| title_sort | valorization of the antioxidant effect of mantua pgi pear by product extracts preparation analysis and biological investigation |
| topic | antioxidant activity circular economy Mantua pear pear extracts supercritical CO<sub>2</sub> extraction |
| url | https://www.mdpi.com/2076-3921/12/1/144 |
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