Assessment of the Effects of Extremely Low Frequency Electromagnetic Fields on Toxoplasma gondii
Background: The effects of extremely low frequency electromagnetic fields (ELF-EMF) on Toxoplasma gondii have not been explained yet. The aim of this study was to assess the possible effects of ELF-EMF on growth, survival time and viability of Toxoplasma gondii. In addition, the life span of Toxopla...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2016-12-01
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| Series: | Iranian Journal of Parasitology |
| Subjects: | |
| Online Access: | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/1055 |
| _version_ | 1798017398924640256 |
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| author | Sercin OZLEM-CALISKAN Hatice ERTABAKLAR Mehmet Dincer BILGIN Sema ERTUG |
| author_facet | Sercin OZLEM-CALISKAN Hatice ERTABAKLAR Mehmet Dincer BILGIN Sema ERTUG |
| author_sort | Sercin OZLEM-CALISKAN |
| collection | DOAJ |
| description | Background: The effects of extremely low frequency electromagnetic fields (ELF-EMF) on Toxoplasma gondii have not been explained yet. The aim of this study was to assess the possible effects of ELF-EMF on growth, survival time and viability of Toxoplasma gondii. In addition, the life span of Toxoplasma infected animals was investigated.
Methods: Sixty adult male BALB/c mice were used for in vivo and in vivo experiments in Laboratory of Biopyhsics and Parasitology of Medical Faculty, Adnan Menderes University, Turkey, in 2010. During in vivo experiments, pulsed and continuous EMFs were applied for 5 d to the infected mice. During in vivo experiments, pulsed and continuous EMF was applied to the tachyzoites within peritoneal exudates for 8 h/d at 4 °C and the tachyzoites were then injected to mice. In both experiments, the number of T. gondii in peritoneal exudates was counted and T. gondii protein bands patterns were investigated with polyacrylamide gel electrophoresis and Western Blotting.
Results: Pulsed and continuous EMF exposure reduced the number of T. gondii tachyzoites in comparison to controls. However, no statistically significant differences were observed at the patterns of protein bands among the samples.
Conclusion: EMF exposure induces a decrease in the number of T. gondii. Further studies are required to understand the mechanism of EMF on intracellular parasites. |
| first_indexed | 2024-04-11T16:06:34Z |
| format | Article |
| id | doaj.art-c59bcd1a276043468437ba0d2c5021b5 |
| institution | Directory Open Access Journal |
| issn | 1735-7020 2008-238X |
| language | English |
| last_indexed | 2024-04-11T16:06:34Z |
| publishDate | 2016-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Parasitology |
| spelling | doaj.art-c59bcd1a276043468437ba0d2c5021b52022-12-22T04:14:48ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2016-12-01112565Assessment of the Effects of Extremely Low Frequency Electromagnetic Fields on Toxoplasma gondiiSercin OZLEM-CALISKAN0Hatice ERTABAKLAR1Mehmet Dincer BILGIN2Sema ERTUG3Department of Biophysics, Institute of Health Sciences, Adnan Menderes University, Aydin, TurkeyDepartment of Parasitology, Medical Faculty, Adnan Menderes University, Aydin, TurkeyDepartment of Biophysics, Institute of Health Sciences, Adnan Menderes University, Aydin, Turkey AND Department of Biophysics, Medical Faculty, Adnan Menderes University, Aydin, TurkeyDepartment of Parasitology, Medical Faculty, Adnan Menderes University, Aydin, TurkeyBackground: The effects of extremely low frequency electromagnetic fields (ELF-EMF) on Toxoplasma gondii have not been explained yet. The aim of this study was to assess the possible effects of ELF-EMF on growth, survival time and viability of Toxoplasma gondii. In addition, the life span of Toxoplasma infected animals was investigated. Methods: Sixty adult male BALB/c mice were used for in vivo and in vivo experiments in Laboratory of Biopyhsics and Parasitology of Medical Faculty, Adnan Menderes University, Turkey, in 2010. During in vivo experiments, pulsed and continuous EMFs were applied for 5 d to the infected mice. During in vivo experiments, pulsed and continuous EMF was applied to the tachyzoites within peritoneal exudates for 8 h/d at 4 °C and the tachyzoites were then injected to mice. In both experiments, the number of T. gondii in peritoneal exudates was counted and T. gondii protein bands patterns were investigated with polyacrylamide gel electrophoresis and Western Blotting. Results: Pulsed and continuous EMF exposure reduced the number of T. gondii tachyzoites in comparison to controls. However, no statistically significant differences were observed at the patterns of protein bands among the samples. Conclusion: EMF exposure induces a decrease in the number of T. gondii. Further studies are required to understand the mechanism of EMF on intracellular parasites.https://ijpa.tums.ac.ir/index.php/ijpa/article/view/1055Electromagnetic fieldMicePolyacrylamide gel elec-trophoresisToxoplasma gondiiWestern Blotting |
| spellingShingle | Sercin OZLEM-CALISKAN Hatice ERTABAKLAR Mehmet Dincer BILGIN Sema ERTUG Assessment of the Effects of Extremely Low Frequency Electromagnetic Fields on Toxoplasma gondii Iranian Journal of Parasitology Electromagnetic field Mice Polyacrylamide gel elec-trophoresis Toxoplasma gondii Western Blotting |
| title | Assessment of the Effects of Extremely Low Frequency Electromagnetic Fields on Toxoplasma gondii |
| title_full | Assessment of the Effects of Extremely Low Frequency Electromagnetic Fields on Toxoplasma gondii |
| title_fullStr | Assessment of the Effects of Extremely Low Frequency Electromagnetic Fields on Toxoplasma gondii |
| title_full_unstemmed | Assessment of the Effects of Extremely Low Frequency Electromagnetic Fields on Toxoplasma gondii |
| title_short | Assessment of the Effects of Extremely Low Frequency Electromagnetic Fields on Toxoplasma gondii |
| title_sort | assessment of the effects of extremely low frequency electromagnetic fields on toxoplasma gondii |
| topic | Electromagnetic field Mice Polyacrylamide gel elec-trophoresis Toxoplasma gondii Western Blotting |
| url | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/1055 |
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