Qualitative Analysis of Multiple Phytochemical Compounds in Tojapride Based on UHPLC Q-Exactive Orbitrap Mass Spectrometry
Tojapride is composed of Caulis Perillae, Rhizoma Cyperi, Radix Glycyrrhizae, <i>Citrus aurantium</i> L., <i>Coptis chinensis</i> Franch, Pericarpium Citri Reticulatae, <i>Reynoutria japonica</i> Houtt, <i>Tetradium ruticarpum</i>, and <i>Cleisto...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/19/6639 |
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| author | Liying Zhang Shihan Qin Sunv Tang Shuai E Kailin Li Jing Li Wei Cai Lei Sun Hui Li |
| author_facet | Liying Zhang Shihan Qin Sunv Tang Shuai E Kailin Li Jing Li Wei Cai Lei Sun Hui Li |
| author_sort | Liying Zhang |
| collection | DOAJ |
| description | Tojapride is composed of Caulis Perillae, Rhizoma Cyperi, Radix Glycyrrhizae, <i>Citrus aurantium</i> L., <i>Coptis chinensis</i> Franch, Pericarpium Citri Reticulatae, <i>Reynoutria japonica</i> Houtt, <i>Tetradium ruticarpum</i>, and <i>Cleistocactus sepium</i>. It has the effects of inhibiting gastric acid and relieving pain. It is clinically used for treating gastroesophageal reflux disease. To further study the pharmacodynamic properties of Tojapride, the systematic characterization of the chemical constituents in Tojapride was investigated using ultra-performance liquid chromatography with Q-Exactive Orbitrap mass spectrometry combined with parallel reaction monitoring for the first time. Eventually, a total of 222 compounds, including flavonoids, alkaloids, and glycyrrhizic acid derivatives, were identified based on the chromatographic retention times, MS/MS<sup>2</sup> information, and bibliography data; a total of 218 of these were reported for the first time as being present in Tojapride. This newly developed approach provides a powerful tool for extending our understanding of chemical constituents of Tojapride, which can be further extended to other TCMP composition research. |
| first_indexed | 2024-03-09T21:24:04Z |
| format | Article |
| id | doaj.art-c5a29b528eca47b68daec1435fb9241b |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T21:24:04Z |
| publishDate | 2022-10-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-c5a29b528eca47b68daec1435fb9241b2023-11-23T21:14:45ZengMDPI AGMolecules1420-30492022-10-012719663910.3390/molecules27196639Qualitative Analysis of Multiple Phytochemical Compounds in Tojapride Based on UHPLC Q-Exactive Orbitrap Mass SpectrometryLiying Zhang0Shihan Qin1Sunv Tang2Shuai E3Kailin Li4Jing Li5Wei Cai6Lei Sun7Hui Li8Institute of Digestive Diseases, Xiyuan Hospital of China Academy of Chinese Medical Sciences, Beijing 100000, ChinaSchool of Pharmaceutical Sciences, Hunan University of Medicine, Huaihua 418000, ChinaSchool of Pharmaceutical Sciences, Hunan University of Medicine, Huaihua 418000, ChinaSchool of Pharmaceutical Sciences, Hunan University of Medicine, Huaihua 418000, ChinaSchool of Pharmaceutical Sciences, Hunan University of Medicine, Huaihua 418000, ChinaSchool of Pharmaceutical Sciences, Hunan University of Medicine, Huaihua 418000, ChinaSchool of Pharmaceutical Sciences, Hunan University of Medicine, Huaihua 418000, ChinaNational Institutes for Food and Drug Control, Beijing 100050, ChinaSchool of Pharmaceutical Sciences, Hunan University of Medicine, Huaihua 418000, ChinaTojapride is composed of Caulis Perillae, Rhizoma Cyperi, Radix Glycyrrhizae, <i>Citrus aurantium</i> L., <i>Coptis chinensis</i> Franch, Pericarpium Citri Reticulatae, <i>Reynoutria japonica</i> Houtt, <i>Tetradium ruticarpum</i>, and <i>Cleistocactus sepium</i>. It has the effects of inhibiting gastric acid and relieving pain. It is clinically used for treating gastroesophageal reflux disease. To further study the pharmacodynamic properties of Tojapride, the systematic characterization of the chemical constituents in Tojapride was investigated using ultra-performance liquid chromatography with Q-Exactive Orbitrap mass spectrometry combined with parallel reaction monitoring for the first time. Eventually, a total of 222 compounds, including flavonoids, alkaloids, and glycyrrhizic acid derivatives, were identified based on the chromatographic retention times, MS/MS<sup>2</sup> information, and bibliography data; a total of 218 of these were reported for the first time as being present in Tojapride. This newly developed approach provides a powerful tool for extending our understanding of chemical constituents of Tojapride, which can be further extended to other TCMP composition research.https://www.mdpi.com/1420-3049/27/19/6639Tojapridephytochemical compoundsUHPLC Q-Exactive Orbitrap mass spectrometry |
| spellingShingle | Liying Zhang Shihan Qin Sunv Tang Shuai E Kailin Li Jing Li Wei Cai Lei Sun Hui Li Qualitative Analysis of Multiple Phytochemical Compounds in Tojapride Based on UHPLC Q-Exactive Orbitrap Mass Spectrometry Molecules Tojapride phytochemical compounds UHPLC Q-Exactive Orbitrap mass spectrometry |
| title | Qualitative Analysis of Multiple Phytochemical Compounds in Tojapride Based on UHPLC Q-Exactive Orbitrap Mass Spectrometry |
| title_full | Qualitative Analysis of Multiple Phytochemical Compounds in Tojapride Based on UHPLC Q-Exactive Orbitrap Mass Spectrometry |
| title_fullStr | Qualitative Analysis of Multiple Phytochemical Compounds in Tojapride Based on UHPLC Q-Exactive Orbitrap Mass Spectrometry |
| title_full_unstemmed | Qualitative Analysis of Multiple Phytochemical Compounds in Tojapride Based on UHPLC Q-Exactive Orbitrap Mass Spectrometry |
| title_short | Qualitative Analysis of Multiple Phytochemical Compounds in Tojapride Based on UHPLC Q-Exactive Orbitrap Mass Spectrometry |
| title_sort | qualitative analysis of multiple phytochemical compounds in tojapride based on uhplc q exactive orbitrap mass spectrometry |
| topic | Tojapride phytochemical compounds UHPLC Q-Exactive Orbitrap mass spectrometry |
| url | https://www.mdpi.com/1420-3049/27/19/6639 |
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