The ameliorative role of specific probiotic combinations on bone loss in the ovariectomized rat model
Abstract Background Osteoporosis, a skeletal disease described by impaired bone strength, cause an increased risk of fractures. We aimed in this study to clarify which particular wise combination of probiotics has the most beneficial effect in the rat model of osteoporosis. Methods Sixty-three matur...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-09-01
|
| Series: | BMC Complementary Medicine and Therapies |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12906-022-03713-y |
| _version_ | 1828238194044305408 |
|---|---|
| author | Ahmad Gholami Mohammad Hossein Dabbaghmanesh Younes Ghasemi Farhad Koohpeyma Pedram Talezadeh Nima Montazeri-Najafabady |
| author_facet | Ahmad Gholami Mohammad Hossein Dabbaghmanesh Younes Ghasemi Farhad Koohpeyma Pedram Talezadeh Nima Montazeri-Najafabady |
| author_sort | Ahmad Gholami |
| collection | DOAJ |
| description | Abstract Background Osteoporosis, a skeletal disease described by impaired bone strength, cause an increased risk of fractures. We aimed in this study to clarify which particular wise combination of probiotics has the most beneficial effect in the rat model of osteoporosis. Methods Sixty-three mature female Sprague Dawley rats (12–14 weeks old, weight 200 ± 20 g) were ovariectomized and then divided into nine random groups, each group consisting of 7 rats. Lactic acid bacteria were isolated from traditional fermented yogurt on the northern coast of the Persian Gulf. Seven combinations of probiotics, each containing three probiotic strains, were designed and administered (1 × 10 9 CFU / ml/strain daily along with their water) to treat ovariectomized rats. The period from ovariectomy to eutanásia was 3 months. For evaluating femur, spine, and tibia, bone mineral density (BMD), and bone mineral content (BMC), Dual-energy X-ray absorptiometry (DEXA) scans were performed. Also, effect of probiotic combinations was assessed on biochemical markers including vitamin D, calcium, phosphorus, and alkaline phosphatase in serum. Results Combination NO 4, containing L. acidophilus, B. longum, and L. reuteri, is the most influential group on global, spine, and femur BMD. Combination NO 3, containing L. acidophilus, L. casei, and L. reuteri, also significantly affects the BMD of the tibia among the treatment group. We found that the combination NO 4 had the most significant ameliorative effect on global BMC. Also, combination NO 1 (comprising L. acidophilus, L. casei, and B. longum), NO 6 (containing L. casei, B. longum, and Bacillus coagulans), NO 7 (containing L. casei, L. reuteri, and B. longum), and NO 4 had the most considerable raising effect on spine BMC. In addition, the serum calcium and Vitamin D concentration in the groups NO 4, 6, and 7 were significantly higher than in OVX groups, whereas the alkaline phosphatase concentration was considerably reduced in these groups. Conclusion Among nine effective probiotics, a combination containing L. acidophilus, B. longum, and L. reuteri is the most influential group in ovariectomized osteoporotic rat. |
| first_indexed | 2024-04-12T20:59:30Z |
| format | Article |
| id | doaj.art-c5a395245d044d0fa0ba880e6c268936 |
| institution | Directory Open Access Journal |
| issn | 2662-7671 |
| language | English |
| last_indexed | 2024-04-12T20:59:30Z |
| publishDate | 2022-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary Medicine and Therapies |
| spelling | doaj.art-c5a395245d044d0fa0ba880e6c2689362022-12-22T03:16:52ZengBMCBMC Complementary Medicine and Therapies2662-76712022-09-0122111110.1186/s12906-022-03713-yThe ameliorative role of specific probiotic combinations on bone loss in the ovariectomized rat modelAhmad Gholami0Mohammad Hossein Dabbaghmanesh1Younes Ghasemi2Farhad Koohpeyma3Pedram Talezadeh4Nima Montazeri-Najafabady5Biotechnology Research Center, Shiraz University of Medical SciencesEndocrinology and Metabolism Research Center, Nemazee Hospital, Shiraz University of Medical SciencesPharmaceutical Science Research Center and School of Pharmacy Shiraz University of Medical SciencesEndocrinology and Metabolism Research Center, Nemazee Hospital, Shiraz University of Medical SciencesEndocrinology and Metabolism Research Center, Nemazee Hospital, Shiraz University of Medical SciencesEndocrinology and Metabolism Research Center, Nemazee Hospital, Shiraz University of Medical SciencesAbstract Background Osteoporosis, a skeletal disease described by impaired bone strength, cause an increased risk of fractures. We aimed in this study to clarify which particular wise combination of probiotics has the most beneficial effect in the rat model of osteoporosis. Methods Sixty-three mature female Sprague Dawley rats (12–14 weeks old, weight 200 ± 20 g) were ovariectomized and then divided into nine random groups, each group consisting of 7 rats. Lactic acid bacteria were isolated from traditional fermented yogurt on the northern coast of the Persian Gulf. Seven combinations of probiotics, each containing three probiotic strains, were designed and administered (1 × 10 9 CFU / ml/strain daily along with their water) to treat ovariectomized rats. The period from ovariectomy to eutanásia was 3 months. For evaluating femur, spine, and tibia, bone mineral density (BMD), and bone mineral content (BMC), Dual-energy X-ray absorptiometry (DEXA) scans were performed. Also, effect of probiotic combinations was assessed on biochemical markers including vitamin D, calcium, phosphorus, and alkaline phosphatase in serum. Results Combination NO 4, containing L. acidophilus, B. longum, and L. reuteri, is the most influential group on global, spine, and femur BMD. Combination NO 3, containing L. acidophilus, L. casei, and L. reuteri, also significantly affects the BMD of the tibia among the treatment group. We found that the combination NO 4 had the most significant ameliorative effect on global BMC. Also, combination NO 1 (comprising L. acidophilus, L. casei, and B. longum), NO 6 (containing L. casei, B. longum, and Bacillus coagulans), NO 7 (containing L. casei, L. reuteri, and B. longum), and NO 4 had the most considerable raising effect on spine BMC. In addition, the serum calcium and Vitamin D concentration in the groups NO 4, 6, and 7 were significantly higher than in OVX groups, whereas the alkaline phosphatase concentration was considerably reduced in these groups. Conclusion Among nine effective probiotics, a combination containing L. acidophilus, B. longum, and L. reuteri is the most influential group in ovariectomized osteoporotic rat.https://doi.org/10.1186/s12906-022-03713-yProbioticOsteoporosisLactobacillusBone |
| spellingShingle | Ahmad Gholami Mohammad Hossein Dabbaghmanesh Younes Ghasemi Farhad Koohpeyma Pedram Talezadeh Nima Montazeri-Najafabady The ameliorative role of specific probiotic combinations on bone loss in the ovariectomized rat model BMC Complementary Medicine and Therapies Probiotic Osteoporosis Lactobacillus Bone |
| title | The ameliorative role of specific probiotic combinations on bone loss in the ovariectomized rat model |
| title_full | The ameliorative role of specific probiotic combinations on bone loss in the ovariectomized rat model |
| title_fullStr | The ameliorative role of specific probiotic combinations on bone loss in the ovariectomized rat model |
| title_full_unstemmed | The ameliorative role of specific probiotic combinations on bone loss in the ovariectomized rat model |
| title_short | The ameliorative role of specific probiotic combinations on bone loss in the ovariectomized rat model |
| title_sort | ameliorative role of specific probiotic combinations on bone loss in the ovariectomized rat model |
| topic | Probiotic Osteoporosis Lactobacillus Bone |
| url | https://doi.org/10.1186/s12906-022-03713-y |
| work_keys_str_mv | AT ahmadgholami theameliorativeroleofspecificprobioticcombinationsonbonelossintheovariectomizedratmodel AT mohammadhosseindabbaghmanesh theameliorativeroleofspecificprobioticcombinationsonbonelossintheovariectomizedratmodel AT younesghasemi theameliorativeroleofspecificprobioticcombinationsonbonelossintheovariectomizedratmodel AT farhadkoohpeyma theameliorativeroleofspecificprobioticcombinationsonbonelossintheovariectomizedratmodel AT pedramtalezadeh theameliorativeroleofspecificprobioticcombinationsonbonelossintheovariectomizedratmodel AT nimamontazerinajafabady theameliorativeroleofspecificprobioticcombinationsonbonelossintheovariectomizedratmodel AT ahmadgholami ameliorativeroleofspecificprobioticcombinationsonbonelossintheovariectomizedratmodel AT mohammadhosseindabbaghmanesh ameliorativeroleofspecificprobioticcombinationsonbonelossintheovariectomizedratmodel AT younesghasemi ameliorativeroleofspecificprobioticcombinationsonbonelossintheovariectomizedratmodel AT farhadkoohpeyma ameliorativeroleofspecificprobioticcombinationsonbonelossintheovariectomizedratmodel AT pedramtalezadeh ameliorativeroleofspecificprobioticcombinationsonbonelossintheovariectomizedratmodel AT nimamontazerinajafabady ameliorativeroleofspecificprobioticcombinationsonbonelossintheovariectomizedratmodel |