Endophytic bacteria of Fagonia indica Burm. f revealed to harbour rich secondary antibacterial metabolites
Endophytic bacteria are the source of novel bioactive compounds, used as therapeutic agent. Molecular docking is a computational technique use frequently, to find novel drugs targets and drugs-receptors interactions. The current study was designed to isolate and identify endophytic bacteria for the...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9754247/?tool=EBI |
| _version_ | 1797979765967159296 |
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| author | Lubna Rahman Asma Mukhtar Sajjad Ahmad Lutfur Rahman Muhammad Ali Muhammad Saeed Zabta Khan Shinwari |
| author_facet | Lubna Rahman Asma Mukhtar Sajjad Ahmad Lutfur Rahman Muhammad Ali Muhammad Saeed Zabta Khan Shinwari |
| author_sort | Lubna Rahman |
| collection | DOAJ |
| description | Endophytic bacteria are the source of novel bioactive compounds, used as therapeutic agent. Molecular docking is a computational technique use frequently, to find novel drugs targets and drugs-receptors interactions. The current study was designed to isolate and identify endophytic bacteria for the extraction of bioactive compounds. Further, to characterized extracts and to explore compounds interactions with bacterial cell wall and outer membrane synthesizing proteins. Endophytes were identified using 16s rRNA amplification technique. For bioactive compounds, solvent extraction method was followed and characterized further through GC-MS analysis. To find targets and drugs-receptors interactions, molecular docking studies and biological assays were performed. The isolated endophytes belong to five different genera namely Enterobacter, Bacillus, Erwinia, Stenotrophomonas and Pantoea. In case of antibacterial assay Stenotrophomonas maltophilia extract showed significant inhibitory zones (15.11±0.11mm and 11.3±0.16) against Staphylococcus caseolyticus and Acinetobacter baumanni, with MIC 33.3 and 50μg/mL respectively. Among the characterized fifty compounds, from endophytic bacteria “antibacterial compound” N-(5-benzyl-10b-hydroxy-2-methyl-3,6-dioxooctahydro-8H-oxazolo[3,2-α] pyrrolo[2,1c] pyrazin-2-yl)-7-methyl2,3,3a,3a1,6,6a,7,8,9,10,10a,10b-dodecahydro-1H-4λ2-indolo[4,3-fg]quinoline-9-carboxamide of bacteria Stenotrophomonas maltophilia were an excellent binder with MurF ligase active site, with binding energy of -10.2 kcal/mol. Extracts of endophytic bacteria composed of various pharmacologically active ingredients such as antibacterial compounds. Molecular docking studies provide important information regarding drug-receptor interaction, thus can be used in novel drug discovery. |
| first_indexed | 2024-04-11T05:44:51Z |
| format | Article |
| id | doaj.art-c5a6180fc8f24cf38c08bac189540e5b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-11T05:44:51Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c5a6180fc8f24cf38c08bac189540e5b2022-12-22T04:42:18ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-011712Endophytic bacteria of Fagonia indica Burm. f revealed to harbour rich secondary antibacterial metabolitesLubna RahmanAsma MukhtarSajjad AhmadLutfur RahmanMuhammad AliMuhammad SaeedZabta Khan ShinwariEndophytic bacteria are the source of novel bioactive compounds, used as therapeutic agent. Molecular docking is a computational technique use frequently, to find novel drugs targets and drugs-receptors interactions. The current study was designed to isolate and identify endophytic bacteria for the extraction of bioactive compounds. Further, to characterized extracts and to explore compounds interactions with bacterial cell wall and outer membrane synthesizing proteins. Endophytes were identified using 16s rRNA amplification technique. For bioactive compounds, solvent extraction method was followed and characterized further through GC-MS analysis. To find targets and drugs-receptors interactions, molecular docking studies and biological assays were performed. The isolated endophytes belong to five different genera namely Enterobacter, Bacillus, Erwinia, Stenotrophomonas and Pantoea. In case of antibacterial assay Stenotrophomonas maltophilia extract showed significant inhibitory zones (15.11±0.11mm and 11.3±0.16) against Staphylococcus caseolyticus and Acinetobacter baumanni, with MIC 33.3 and 50μg/mL respectively. Among the characterized fifty compounds, from endophytic bacteria “antibacterial compound” N-(5-benzyl-10b-hydroxy-2-methyl-3,6-dioxooctahydro-8H-oxazolo[3,2-α] pyrrolo[2,1c] pyrazin-2-yl)-7-methyl2,3,3a,3a1,6,6a,7,8,9,10,10a,10b-dodecahydro-1H-4λ2-indolo[4,3-fg]quinoline-9-carboxamide of bacteria Stenotrophomonas maltophilia were an excellent binder with MurF ligase active site, with binding energy of -10.2 kcal/mol. Extracts of endophytic bacteria composed of various pharmacologically active ingredients such as antibacterial compounds. Molecular docking studies provide important information regarding drug-receptor interaction, thus can be used in novel drug discovery.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9754247/?tool=EBI |
| spellingShingle | Lubna Rahman Asma Mukhtar Sajjad Ahmad Lutfur Rahman Muhammad Ali Muhammad Saeed Zabta Khan Shinwari Endophytic bacteria of Fagonia indica Burm. f revealed to harbour rich secondary antibacterial metabolites PLoS ONE |
| title | Endophytic bacteria of Fagonia indica Burm. f revealed to harbour rich secondary antibacterial metabolites |
| title_full | Endophytic bacteria of Fagonia indica Burm. f revealed to harbour rich secondary antibacterial metabolites |
| title_fullStr | Endophytic bacteria of Fagonia indica Burm. f revealed to harbour rich secondary antibacterial metabolites |
| title_full_unstemmed | Endophytic bacteria of Fagonia indica Burm. f revealed to harbour rich secondary antibacterial metabolites |
| title_short | Endophytic bacteria of Fagonia indica Burm. f revealed to harbour rich secondary antibacterial metabolites |
| title_sort | endophytic bacteria of fagonia indica burm f revealed to harbour rich secondary antibacterial metabolites |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9754247/?tool=EBI |
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