Domoic Acid Oxidative Effects on the Microalgae <i>Phaeodactylum tricornutum</i>
Domoic acid (DA) is a natural occurring marine biotoxin. Oxidative stress generation due to DA exposure was reported in animals, but little is known on the phytoplankton community. The aim of this work was to verify whether exposure to DA in the marine diatom <i>Phaeodactylum tricornutum</i...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/13/3/676 |
| _version_ | 1797610677758590976 |
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| author | Joaquin Cabrera Susana Puntarulo Paula Mariela González |
| author_facet | Joaquin Cabrera Susana Puntarulo Paula Mariela González |
| author_sort | Joaquin Cabrera |
| collection | DOAJ |
| description | Domoic acid (DA) is a natural occurring marine biotoxin. Oxidative stress generation due to DA exposure was reported in animals, but little is known on the phytoplankton community. The aim of this work was to verify whether exposure to DA in the marine diatom <i>Phaeodactylum tricornutum</i> favors reactive oxygen species (ROS) generation in the intracellular environment modifying its antioxidant capacity. Active species production, non-enzymatic antioxidant content, and antioxidant enzyme activities over the three growth phases of <i>P. tricornutum</i> exposed to 64 µM DA were evaluated. Results obtained in exponential growing cells showed a time-depending seven-fold increase in the 2′,7′ dichlorofluorescein diacetate dye oxidation rate. Superoxide dismutase and catalase activities showed a two-fold increase, and glutathione related enzymes activities were also significantly increased in treated diatoms as compared to controls. However, glutathione and ascorbate contents significantly decreased after incubation of the cells with DA. Similar effects were observed in latent and stationary phases of cell development. These results showed that DA could cause a severe oxidant-dependent impact on a non-toxic algae. |
| first_indexed | 2024-03-11T06:17:37Z |
| format | Article |
| id | doaj.art-c5a96db20b5a4c1399f67c899a388707 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-11T06:17:37Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-c5a96db20b5a4c1399f67c899a3887072023-11-17T12:10:50ZengMDPI AGLife2075-17292023-03-0113367610.3390/life13030676Domoic Acid Oxidative Effects on the Microalgae <i>Phaeodactylum tricornutum</i>Joaquin Cabrera0Susana Puntarulo1Paula Mariela González2Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Fisicoquímica, Buenos Aires CP 1113, ArgentinaFacultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Fisicoquímica, Buenos Aires CP 1113, ArgentinaFacultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Fisicoquímica, Buenos Aires CP 1113, ArgentinaDomoic acid (DA) is a natural occurring marine biotoxin. Oxidative stress generation due to DA exposure was reported in animals, but little is known on the phytoplankton community. The aim of this work was to verify whether exposure to DA in the marine diatom <i>Phaeodactylum tricornutum</i> favors reactive oxygen species (ROS) generation in the intracellular environment modifying its antioxidant capacity. Active species production, non-enzymatic antioxidant content, and antioxidant enzyme activities over the three growth phases of <i>P. tricornutum</i> exposed to 64 µM DA were evaluated. Results obtained in exponential growing cells showed a time-depending seven-fold increase in the 2′,7′ dichlorofluorescein diacetate dye oxidation rate. Superoxide dismutase and catalase activities showed a two-fold increase, and glutathione related enzymes activities were also significantly increased in treated diatoms as compared to controls. However, glutathione and ascorbate contents significantly decreased after incubation of the cells with DA. Similar effects were observed in latent and stationary phases of cell development. These results showed that DA could cause a severe oxidant-dependent impact on a non-toxic algae.https://www.mdpi.com/2075-1729/13/3/676allelopathyantioxidantsbiotoxindiatomsharmful algae bloomsoxidative stress |
| spellingShingle | Joaquin Cabrera Susana Puntarulo Paula Mariela González Domoic Acid Oxidative Effects on the Microalgae <i>Phaeodactylum tricornutum</i> Life allelopathy antioxidants biotoxin diatoms harmful algae blooms oxidative stress |
| title | Domoic Acid Oxidative Effects on the Microalgae <i>Phaeodactylum tricornutum</i> |
| title_full | Domoic Acid Oxidative Effects on the Microalgae <i>Phaeodactylum tricornutum</i> |
| title_fullStr | Domoic Acid Oxidative Effects on the Microalgae <i>Phaeodactylum tricornutum</i> |
| title_full_unstemmed | Domoic Acid Oxidative Effects on the Microalgae <i>Phaeodactylum tricornutum</i> |
| title_short | Domoic Acid Oxidative Effects on the Microalgae <i>Phaeodactylum tricornutum</i> |
| title_sort | domoic acid oxidative effects on the microalgae i phaeodactylum tricornutum i |
| topic | allelopathy antioxidants biotoxin diatoms harmful algae blooms oxidative stress |
| url | https://www.mdpi.com/2075-1729/13/3/676 |
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