Revealing the Role of the Calcineurin B-Like Protein-Interacting Protein Kinase 9 (CIPK9) in Rice Adaptive Responses to Salinity, Osmotic Stress, and K<sup>+</sup> Deficiency
In plants, calcineurin B-like (CBL) proteins and their interacting protein kinases (CIPK) form functional complexes that transduce downstream signals to membrane effectors assisting in their adaptation to adverse environmental conditions. This study addresses the issue of the physiological role of C...
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| Format: | Article |
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MDPI AG
2021-07-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/8/1513 |
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| author | Sergey Shabala Mohammad Alnayef Jayakumar Bose Zhong-Hua Chen Gayatri Venkataraman Meixue Zhou Lana Shabala Min Yu |
| author_facet | Sergey Shabala Mohammad Alnayef Jayakumar Bose Zhong-Hua Chen Gayatri Venkataraman Meixue Zhou Lana Shabala Min Yu |
| author_sort | Sergey Shabala |
| collection | DOAJ |
| description | In plants, calcineurin B-like (CBL) proteins and their interacting protein kinases (CIPK) form functional complexes that transduce downstream signals to membrane effectors assisting in their adaptation to adverse environmental conditions. This study addresses the issue of the physiological role of CIPK9 in adaptive responses to salinity, osmotic stress, and K<sup>+</sup> deficiency in rice plants. Whole-plant physiological studies revealed that <i>Oscipk9</i> rice mutant lacks a functional CIPK9 gene and displayed a mildly stronger phenotype, both under saline and osmotic stress conditions. The reported difference was attributed to the ability of <i>Oscipk9</i> to maintain significantly higher stomatal conductance (thus, a greater carbon gain). <i>Oscipk9</i> plants contained much less K<sup>+</sup> in their tissues, implying the role of CIPK9 in K<sup>+</sup> acquisition and homeostasis in rice. <i>Oscipk9</i> roots also showed hypersensitivity to ROS under conditions of low K<sup>+</sup> availability suggesting an important role of H<sub>2</sub>O<sub>2</sub> signalling as a component of plant adaptive responses to a low-K environment. The likely mechanistic basis of above physiological responses is discussed. |
| first_indexed | 2024-03-10T08:28:40Z |
| format | Article |
| id | doaj.art-c5a9b5623a6a49ad8cd5dc3d8938e2e9 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T08:28:40Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-c5a9b5623a6a49ad8cd5dc3d8938e2e92023-11-22T09:17:26ZengMDPI AGPlants2223-77472021-07-01108151310.3390/plants10081513Revealing the Role of the Calcineurin B-Like Protein-Interacting Protein Kinase 9 (CIPK9) in Rice Adaptive Responses to Salinity, Osmotic Stress, and K<sup>+</sup> DeficiencySergey Shabala0Mohammad Alnayef1Jayakumar Bose2Zhong-Hua Chen3Gayatri Venkataraman4Meixue Zhou5Lana Shabala6Min Yu7International Research Centre for Environmental Membrane Biology, Foshan University, Foshan 528000, ChinaTasmanian Institute of Agriculture, University of Tasmania, Hobart, TAS 7005, AustraliaAustralian Research Council Centre of Excellence in Plant Energy Biology, School of Agriculture, Food and Wine, University of Adelaide, Glen Osmond, SA 5064, AustraliaHawkesbury Institute for the Environment, Western Sydney University, Penrith, NSW 2751, AustraliaPlant Molecular Biology Laboratory, M.S. Swaminathan Research Foundation, Chennai 600113, IndiaTasmanian Institute of Agriculture, University of Tasmania, Hobart, TAS 7005, AustraliaTasmanian Institute of Agriculture, University of Tasmania, Hobart, TAS 7005, AustraliaInternational Research Centre for Environmental Membrane Biology, Foshan University, Foshan 528000, ChinaIn plants, calcineurin B-like (CBL) proteins and their interacting protein kinases (CIPK) form functional complexes that transduce downstream signals to membrane effectors assisting in their adaptation to adverse environmental conditions. This study addresses the issue of the physiological role of CIPK9 in adaptive responses to salinity, osmotic stress, and K<sup>+</sup> deficiency in rice plants. Whole-plant physiological studies revealed that <i>Oscipk9</i> rice mutant lacks a functional CIPK9 gene and displayed a mildly stronger phenotype, both under saline and osmotic stress conditions. The reported difference was attributed to the ability of <i>Oscipk9</i> to maintain significantly higher stomatal conductance (thus, a greater carbon gain). <i>Oscipk9</i> plants contained much less K<sup>+</sup> in their tissues, implying the role of CIPK9 in K<sup>+</sup> acquisition and homeostasis in rice. <i>Oscipk9</i> roots also showed hypersensitivity to ROS under conditions of low K<sup>+</sup> availability suggesting an important role of H<sub>2</sub>O<sub>2</sub> signalling as a component of plant adaptive responses to a low-K environment. The likely mechanistic basis of above physiological responses is discussed.https://www.mdpi.com/2223-7747/10/8/1513calcium signallingpotassium transportAKTHAKreactive oxygen speciesABA |
| spellingShingle | Sergey Shabala Mohammad Alnayef Jayakumar Bose Zhong-Hua Chen Gayatri Venkataraman Meixue Zhou Lana Shabala Min Yu Revealing the Role of the Calcineurin B-Like Protein-Interacting Protein Kinase 9 (CIPK9) in Rice Adaptive Responses to Salinity, Osmotic Stress, and K<sup>+</sup> Deficiency Plants calcium signalling potassium transport AKT HAK reactive oxygen species ABA |
| title | Revealing the Role of the Calcineurin B-Like Protein-Interacting Protein Kinase 9 (CIPK9) in Rice Adaptive Responses to Salinity, Osmotic Stress, and K<sup>+</sup> Deficiency |
| title_full | Revealing the Role of the Calcineurin B-Like Protein-Interacting Protein Kinase 9 (CIPK9) in Rice Adaptive Responses to Salinity, Osmotic Stress, and K<sup>+</sup> Deficiency |
| title_fullStr | Revealing the Role of the Calcineurin B-Like Protein-Interacting Protein Kinase 9 (CIPK9) in Rice Adaptive Responses to Salinity, Osmotic Stress, and K<sup>+</sup> Deficiency |
| title_full_unstemmed | Revealing the Role of the Calcineurin B-Like Protein-Interacting Protein Kinase 9 (CIPK9) in Rice Adaptive Responses to Salinity, Osmotic Stress, and K<sup>+</sup> Deficiency |
| title_short | Revealing the Role of the Calcineurin B-Like Protein-Interacting Protein Kinase 9 (CIPK9) in Rice Adaptive Responses to Salinity, Osmotic Stress, and K<sup>+</sup> Deficiency |
| title_sort | revealing the role of the calcineurin b like protein interacting protein kinase 9 cipk9 in rice adaptive responses to salinity osmotic stress and k sup sup deficiency |
| topic | calcium signalling potassium transport AKT HAK reactive oxygen species ABA |
| url | https://www.mdpi.com/2223-7747/10/8/1513 |
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