Improving the Thermostability of Acidic Pullulanase from Bacillus naganoensis by Rational Design.

Improving the Thermostability of Acidic Pullulanase from Bacillus naganoensis by Rational Design.

Pullulanase (EC 3.2.1.41) plays an important role in the specific hydrolysis of branch points in amylopectin. Enhancing its thermostability is required for its industrial application. In this study, rational protein design was used to improve the thermostability of PulB from Bacillus naganoensis (AB...

Full description

Bibliographic Details
Main Authors:	Meihui Chang, Xiaoyu Chu, Jinzhi Lv, Qingbin Li, Jian Tian, Ningfeng Wu
Format:	Article
Language:	English
Published:	Public Library of Science (PLoS) 2016-01-01
Series:	PLoS ONE
Online Access:	http://europepmc.org/articles/PMC5072709?pdf=render

Similar Items

Active Hydrogen Bond Network (AHBN) and Applications for Improvement of Thermal Stability and pH-Sensitivity of Pullulanase from Bacillus naganoensis.
by: Qing-Yan Wang, et al.
Published: (2017-01-01)

High-level expression of Bacillus naganoensis pullulanase from recombinant Escherichia coli with auto-induction: effect of lac operator.
by: Yao Nie, et al.
Published: (2013-01-01)

Safety evaluation of the food enzyme pullulanase from Pullulanibacillus naganoensis strain AE‐PL
by: EFSA Panel on Food Contact Materials, Enzymes, Flavourings and Processing Aids (CEF), et al.
Published: (2017-10-01)

Thermostable pullulanase from bacillus flavothermus KWF-1 /
by: Rozaimi Abu Samah, et al.
Published: (2004)

Partial characterization of thermostable pullulanase from bacillus flavothermus KWF-1 /
by: Rozaimi Abu Samah, author, et al.
Published: (2005)

Production purification and characterization of thermostable pullulanase from Bacillus Flavothermus KWF-1 /
by: Nur Aswati Kader Olee, 1984-, et al.
Published: (2011)

Production of thermostable pullulanase from Bacillus Flavothermus KWF-1 in fed-batch culture /
by: Shankar Ramanathan, 1984-, et al.
Published: (2011)

Production of thermostable pullulanase from Bacillus Flavothermus KWF-1 in fed-batch culture
by: Ramanathan, Shankar
Published: (2011)

Production purification and characterization of thermostable pullulanase from Bacillus Flavothermus KWF-1 [electronic resource] /
by: Nur Aswati Kader Olee, 1984-
Published: (2012)

Production of thermostable pullulanase from Bacillus Flavothermus KWF-1 in fed-batch culture [electronic resource] /
by: Shankar Ramanathan, 1984-
Published: (2011)

Enhanced Production of Thermostable Pullulanase Type 1 Using Bacillus cereus FDTA 13 and Its Mutant
by: Subhash U. Nair, et al.
Published: (2006-01-01)

Predicting changes in protein thermostability brought about by single- or multi-site mutations
by: Chu Xiaoyu, et al.
Published: (2010-07-01)

Production of thermostable pullulanase from Bacillus flavothermus KWF-1: medium development using experimental design with data transformation
by: Abu Samah, Rozaimi, et al.
Published: (2008)

Isolation, characterization, and cloning of thermostable pullulanase from Geobacillus stearothermophilus ADM-11
by: Dilara Abbas Bukhari, et al.
Published: (2024-02-01)

Cloning and erxpression if pullulanase agene from locally isolated bacillus
by: Md. Salleh, Madihah, et al.
Published: (2006)

Characterization of pullulanase production from Bacillus Flavothermus KWF-1 /
by: Wan Nooraida Wan Mohamed, author, et al.
Published: (2008)

Cloning and expression of pullulanase gene from locally isolated bacillus SP
by: Nik Mahmood, Nik Azmi, et al.
Published: (2006)

New type of pullulanase from bacillus stearothermophilus and molecular cloning and expression of the gene in bacillus subtilis /
by: 298966 Takashi, Kuriki, et al.

Characterization of pullulanase type II from Bacillus cereus H1.5
by: Hii, Siew Ling, et al.
Published: (2009)

Enhanced Expression of Pullulanase in Bacillus subtilis by New Strong Promoters Mined From Transcriptome Data, Both Alone and in Combination
by: Fanqiang Meng, et al.
Published: (2018-11-01)

Enhancement of pullulanase production from recombinant Bacillus subtilis by optimization of feeding strategy and fermentation conditions
by: Yu Zhang, et al.
Published: (2020-01-01)

Rational Design of Adenylate Kinase Thermostability through Coevolution and Sequence Divergence Analysis
by: Jian Chang, et al.
Published: (2021-03-01)

Safety evaluation of the food enzyme pullulanase from the genetically modified Bacillus licheniformis strain NZYM‐LU
by: EFSA Panel on Food Contact Materials, Enzymes and Processing Aids (CEP), et al.
Published: (2022-06-01)

Improving the thermostability of Pseudoalteromonas Porphyrae κ-carrageenase by rational design and MD simulation
by: Yuyan Sang, et al.
Published: (2024-01-01)

Optimum pullulanase concentration for production of glucose /
by: Muhammad Zulhilmi Mohd. Rosley, et al.
Published: (2010)

Use of pullulanase as a biocatalyst for starch hydrolysis: Part 1. Study of the effect of pullulanase on maize amylopectin starch
by: A. A. Papakhin, et al.
Published: (2022-01-01)

Improving the Thermostability of Serine Protease PB92 from <i>Bacillus alcalophilus</i> via Site-Directed Mutagenesis Based on Semi-Rational Design
by: Huabiao Miao, et al.
Published: (2023-08-01)

Pullulanase Is Necessary for the Efficient Intracellular Growth of Francisella tularensis.
by: Akihiko Uda, et al.
Published: (2016-01-01)

Extraction and Purification of Pullulanase from Local Mushrooms
by: Dalal Bedan
Published: (2023-03-01)

Improvement of thermostable productivity ?-amylase from local isolate Bacillus licheniformis H14.
by: Baghdad Science Journal
Published: (2010-03-01)

Thermostable L2 lipase: enzyme properties and rational design
by: Raja Abdul Rahman, Raja Noor Zaliha
Published: (2012)

Pullulanase type II from a local Bacillus isolate H1.5 : isolation, production and characterization of the enzyme
by: Hii, Siew Ling, et al.
Published: (2004)

Safety evaluation of the food enzyme pullulanase from a genetically modified Bacillus licheniformis (strain DP‐Dzp39)
by: EFSA Panel on Food Contact Materials, Enzymes, Processing Aids (CEP), et al.
Published: (2019-01-01)

Rational Design of Disulfide Bonds Increases Thermostability of a Mesophilic 1,3-1,4-β-Glucanase from Bacillus terquilensis.
by: Chengtuo Niu, et al.
Published: (2016-01-01)

Improving the expression of recombinant pullulanase by increasing mRNA stability in Escherichia coli
by: Tao Li, et al.
Published: (2017-09-01)

Production of thermostable L2 lipase from bacillus sp.
by: Raja Abdul Rahman, Raja Noor Zaliha, et al.
Published: (2007)

Molecular dynamics approach in designing Thermostable Bacillus circulans Xylanase
by: Noorbatcha, Ibrahim Ali, et al.
Published: (2011)

A newly isolated thermostable lipase from Bacillus sp.
by: Mohd Shariff, Fairolniza, et al.
Published: (2011)

High yield recombinant thermostable α-amylase production using an improved <it>Bacillus licheniformis </it>system
by: Shi Gui-Yang, et al.
Published: (2009-10-01)

Production of pullulanase from Bacillus flavothermus KWF-1 in batch culture : preliminary characterization and medium development using experimental design
by: Abu, Rozaimi
Published: (2006)