Gambaran Histopatologi Otak Tikus Akibat Injeksi Trimetyltin sebagai Model Penyakit Alzheimer
Trimethyltin chloride (TMT) is an organotin compound which neurotoxic at limbus system and hippocampus in human and animal. Pathology changes that caused by the induction of TMT is a neurodegenerative disorder such as nerve cell death and cognitive impairment. This study was aimed to observe brain p...
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| Format: | Article |
| Language: | Indonesian |
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Universitas Gadjah Mada
2017-02-01
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| Series: | Jurnal Sain Veteriner |
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| Online Access: | https://jurnal.ugm.ac.id/jsv/article/view/22819 |
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| author | Yuli Purwandari Kristianingrum Sitarina Widyarini Sitarina Widyarini Kurniasih Kurniasih Bambang Sutrisno Bambang Sutrisno Charles Rangga Tabbu Charles Rangga Tabbu Charles Rangga Tabbu Sugiyono Sugiyono |
| author_facet | Yuli Purwandari Kristianingrum Sitarina Widyarini Sitarina Widyarini Kurniasih Kurniasih Bambang Sutrisno Bambang Sutrisno Charles Rangga Tabbu Charles Rangga Tabbu Charles Rangga Tabbu Sugiyono Sugiyono |
| author_sort | Yuli Purwandari Kristianingrum |
| collection | DOAJ |
| description | Trimethyltin chloride (TMT) is an organotin compound which neurotoxic at limbus system and hippocampus in human and animal. Pathology changes that caused by the induction of TMT is a neurodegenerative disorder such as nerve cell death and cognitive impairment. This study was aimed to observe brain pathology induced by TMT with multiple doses for 14, 21 and 28 days after treatment. Twenty seven of Wistar rats, at 2 months of age with weight ranging between 200-300 grams were used and divided randomly into 3 groups (n=9). Group I were injected by trimetyiltin with a dose of 6 mg / kg, group II were injected by
trimetyltin with a dose of 8 mg / kg and group III as control without injection. Observation of brain pathology was done by euthanasia on day 14, 21 and 28 after treatment, three rats each. Cortex and hippocampus of the brain
were observed using Hematoxilin and Eosin staining (HE). All of the research procedure was done with the approval and supervision of Animal Ethics Committee LPPT UGM No. 300/KEC-LPPT/VII/2015. The observation of histopathology of the brain's neuron cells injected by trimetyltin dose of 6 mg/kg and 8 mg/kg body weight was showed increasing cell death of brain neurons in the cortex and hippocampus compared to the control group. The highest cell death was on day 14 in the hippocampus and cortex cerebral on day 21after TMT injection. The neuron cell death characterized by the shrink of brain neurons as well as colored eosinophilic cytoplasm. One way ANOVA statistical analysis showed a significant difference number of neurons cell death
between control and treatment groups. Based on this research, it can be concluded that the trimetyltin injection dose of 6 mg / kg and 8 mg / kg of body weight caused neuron cell death in the brain rats from fourteen day after
treatment, especially in the hippocampus and cortex. |
| first_indexed | 2024-12-12T21:57:53Z |
| format | Article |
| id | doaj.art-c5ae67333a114a28bf5bef17423a2126 |
| institution | Directory Open Access Journal |
| issn | 0126-0421 2407-3733 |
| language | Indonesian |
| last_indexed | 2024-12-12T21:57:53Z |
| publishDate | 2017-02-01 |
| publisher | Universitas Gadjah Mada |
| record_format | Article |
| series | Jurnal Sain Veteriner |
| spelling | doaj.art-c5ae67333a114a28bf5bef17423a21262022-12-22T00:10:36ZindUniversitas Gadjah MadaJurnal Sain Veteriner0126-04212407-37332017-02-01341849110.22146/jsv.2281915540Gambaran Histopatologi Otak Tikus Akibat Injeksi Trimetyltin sebagai Model Penyakit AlzheimerYuli Purwandari Kristianingrum0Sitarina Widyarini Sitarina Widyarini1Kurniasih Kurniasih2Bambang Sutrisno Bambang Sutrisno3Charles Rangga Tabbu Charles Rangga Tabbu Charles Rangga Tabbu4Sugiyono Sugiyono5Departemen Patologi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada, YogyakartaDepartemen Patologi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada, YogyakartaDepartemen Patologi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada, YogyakartaDepartemen Patologi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada, YogyakartaDepartemen Patologi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada, YogyakartaDepartemen Patologi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada, YogyakartaTrimethyltin chloride (TMT) is an organotin compound which neurotoxic at limbus system and hippocampus in human and animal. Pathology changes that caused by the induction of TMT is a neurodegenerative disorder such as nerve cell death and cognitive impairment. This study was aimed to observe brain pathology induced by TMT with multiple doses for 14, 21 and 28 days after treatment. Twenty seven of Wistar rats, at 2 months of age with weight ranging between 200-300 grams were used and divided randomly into 3 groups (n=9). Group I were injected by trimetyiltin with a dose of 6 mg / kg, group II were injected by trimetyltin with a dose of 8 mg / kg and group III as control without injection. Observation of brain pathology was done by euthanasia on day 14, 21 and 28 after treatment, three rats each. Cortex and hippocampus of the brain were observed using Hematoxilin and Eosin staining (HE). All of the research procedure was done with the approval and supervision of Animal Ethics Committee LPPT UGM No. 300/KEC-LPPT/VII/2015. The observation of histopathology of the brain's neuron cells injected by trimetyltin dose of 6 mg/kg and 8 mg/kg body weight was showed increasing cell death of brain neurons in the cortex and hippocampus compared to the control group. The highest cell death was on day 14 in the hippocampus and cortex cerebral on day 21after TMT injection. The neuron cell death characterized by the shrink of brain neurons as well as colored eosinophilic cytoplasm. One way ANOVA statistical analysis showed a significant difference number of neurons cell death between control and treatment groups. Based on this research, it can be concluded that the trimetyltin injection dose of 6 mg / kg and 8 mg / kg of body weight caused neuron cell death in the brain rats from fourteen day after treatment, especially in the hippocampus and cortex.https://jurnal.ugm.ac.id/jsv/article/view/22819Trimetyltinneuron cellhippocampuscognitive |
| spellingShingle | Yuli Purwandari Kristianingrum Sitarina Widyarini Sitarina Widyarini Kurniasih Kurniasih Bambang Sutrisno Bambang Sutrisno Charles Rangga Tabbu Charles Rangga Tabbu Charles Rangga Tabbu Sugiyono Sugiyono Gambaran Histopatologi Otak Tikus Akibat Injeksi Trimetyltin sebagai Model Penyakit Alzheimer Jurnal Sain Veteriner Trimetyltin neuron cell hippocampus cognitive |
| title | Gambaran Histopatologi Otak Tikus Akibat Injeksi Trimetyltin sebagai Model Penyakit Alzheimer |
| title_full | Gambaran Histopatologi Otak Tikus Akibat Injeksi Trimetyltin sebagai Model Penyakit Alzheimer |
| title_fullStr | Gambaran Histopatologi Otak Tikus Akibat Injeksi Trimetyltin sebagai Model Penyakit Alzheimer |
| title_full_unstemmed | Gambaran Histopatologi Otak Tikus Akibat Injeksi Trimetyltin sebagai Model Penyakit Alzheimer |
| title_short | Gambaran Histopatologi Otak Tikus Akibat Injeksi Trimetyltin sebagai Model Penyakit Alzheimer |
| title_sort | gambaran histopatologi otak tikus akibat injeksi trimetyltin sebagai model penyakit alzheimer |
| topic | Trimetyltin neuron cell hippocampus cognitive |
| url | https://jurnal.ugm.ac.id/jsv/article/view/22819 |
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