<i>Slc7a8</i> Deletion Is Protective against Diet-Induced Obesity and Attenuates Lipid Accumulation in Multiple Organs
Adipogenesis, through adipocyte hyperplasia and/or hypertrophy, leads to increased adiposity, giving rise to obesity. A genome-wide transcriptome analysis of in vitro adipogenesis in human adipose-derived stromal/stem cells identified SLC7A8 (Solute Carrier Family 7 Member 8) as a potential novel me...
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| Format: | Article |
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MDPI AG
2022-02-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/11/2/311 |
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| author | Reabetswe R. Pitere Marlene B. van Heerden Michael S. Pepper Melvin A. Ambele |
| author_facet | Reabetswe R. Pitere Marlene B. van Heerden Michael S. Pepper Melvin A. Ambele |
| author_sort | Reabetswe R. Pitere |
| collection | DOAJ |
| description | Adipogenesis, through adipocyte hyperplasia and/or hypertrophy, leads to increased adiposity, giving rise to obesity. A genome-wide transcriptome analysis of in vitro adipogenesis in human adipose-derived stromal/stem cells identified SLC7A8 (Solute Carrier Family 7 Member 8) as a potential novel mediator. The current study has investigated the role of SLC7A8 in adipose tissue biology using a mouse model of diet-induced obesity. <i>slc7a8</i> knockout (KO) and wildtype (WT) C57BL/6J mice were fed either a control diet (CD) or a high-fat diet (HFD) for 14 weeks. On the HFD, both WT and KO mice (WTHFD and KOHFD) gained significantly more weight than their CD counterparts. However, KOHFD gained significantly less weight than WTHFD. KOHFD had significantly reduced levels of glucose intolerance compared with those observed in WTHFD. KOHFD also had significantly reduced adipocyte mass and hypertrophy in inguinal, mesenteric, perigonadal, and brown adipose depots, with a corresponding decrease in macrophage infiltration. Additionally, KOHFD had decreased lipid accumulation in the liver, heart, gastrocnemius muscle, lung, and kidney. This study demonstrates that targeting <i>slc7a8</i> protects against diet-induced obesity by reducing lipid accumulation in multiple organs and suggests that if targeted, has the potential to mitigate the development of obesity-associated comorbidities. |
| first_indexed | 2024-03-09T22:34:09Z |
| format | Article |
| id | doaj.art-c5ae7a412d4f4c2484013331f387920d |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-09T22:34:09Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-c5ae7a412d4f4c2484013331f387920d2023-11-23T18:51:16ZengMDPI AGBiology2079-77372022-02-0111231110.3390/biology11020311<i>Slc7a8</i> Deletion Is Protective against Diet-Induced Obesity and Attenuates Lipid Accumulation in Multiple OrgansReabetswe R. Pitere0Marlene B. van Heerden1Michael S. Pepper2Melvin A. Ambele3Institute for Cellular and Molecular Medicine, Department of Immunology and SAMRC Extramural Unit for Stem Cell Research and Therapy, Faculty of Health Sciences, University of Pretoria, Pretoria 0001, South AfricaDepartment of Oral Pathology and Oral Biology, School of Dentistry, Faculty of Health Sciences, University of Pretoria, Pretoria 0001, South AfricaInstitute for Cellular and Molecular Medicine, Department of Immunology and SAMRC Extramural Unit for Stem Cell Research and Therapy, Faculty of Health Sciences, University of Pretoria, Pretoria 0001, South AfricaInstitute for Cellular and Molecular Medicine, Department of Immunology and SAMRC Extramural Unit for Stem Cell Research and Therapy, Faculty of Health Sciences, University of Pretoria, Pretoria 0001, South AfricaAdipogenesis, through adipocyte hyperplasia and/or hypertrophy, leads to increased adiposity, giving rise to obesity. A genome-wide transcriptome analysis of in vitro adipogenesis in human adipose-derived stromal/stem cells identified SLC7A8 (Solute Carrier Family 7 Member 8) as a potential novel mediator. The current study has investigated the role of SLC7A8 in adipose tissue biology using a mouse model of diet-induced obesity. <i>slc7a8</i> knockout (KO) and wildtype (WT) C57BL/6J mice were fed either a control diet (CD) or a high-fat diet (HFD) for 14 weeks. On the HFD, both WT and KO mice (WTHFD and KOHFD) gained significantly more weight than their CD counterparts. However, KOHFD gained significantly less weight than WTHFD. KOHFD had significantly reduced levels of glucose intolerance compared with those observed in WTHFD. KOHFD also had significantly reduced adipocyte mass and hypertrophy in inguinal, mesenteric, perigonadal, and brown adipose depots, with a corresponding decrease in macrophage infiltration. Additionally, KOHFD had decreased lipid accumulation in the liver, heart, gastrocnemius muscle, lung, and kidney. This study demonstrates that targeting <i>slc7a8</i> protects against diet-induced obesity by reducing lipid accumulation in multiple organs and suggests that if targeted, has the potential to mitigate the development of obesity-associated comorbidities.https://www.mdpi.com/2079-7737/11/2/311obesityadipose tissueadipogenesislipid accumulationSLC7A8fat depots |
| spellingShingle | Reabetswe R. Pitere Marlene B. van Heerden Michael S. Pepper Melvin A. Ambele <i>Slc7a8</i> Deletion Is Protective against Diet-Induced Obesity and Attenuates Lipid Accumulation in Multiple Organs Biology obesity adipose tissue adipogenesis lipid accumulation SLC7A8 fat depots |
| title | <i>Slc7a8</i> Deletion Is Protective against Diet-Induced Obesity and Attenuates Lipid Accumulation in Multiple Organs |
| title_full | <i>Slc7a8</i> Deletion Is Protective against Diet-Induced Obesity and Attenuates Lipid Accumulation in Multiple Organs |
| title_fullStr | <i>Slc7a8</i> Deletion Is Protective against Diet-Induced Obesity and Attenuates Lipid Accumulation in Multiple Organs |
| title_full_unstemmed | <i>Slc7a8</i> Deletion Is Protective against Diet-Induced Obesity and Attenuates Lipid Accumulation in Multiple Organs |
| title_short | <i>Slc7a8</i> Deletion Is Protective against Diet-Induced Obesity and Attenuates Lipid Accumulation in Multiple Organs |
| title_sort | i slc7a8 i deletion is protective against diet induced obesity and attenuates lipid accumulation in multiple organs |
| topic | obesity adipose tissue adipogenesis lipid accumulation SLC7A8 fat depots |
| url | https://www.mdpi.com/2079-7737/11/2/311 |
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