Emerging applications at the interface of DNA nanotechnology and cellular membranes: Perspectives from biology, engineering, and physics
DNA nanotechnology has proven exceptionally apt at probing and manipulating biological environments as it can create nanostructures of almost arbitrary shape that permit countless types of modifications, all while being inherently biocompatible. Emergent areas of particular interest are applications...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
AIP Publishing LLC
2020-12-01
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| Series: | APL Bioengineering |
| Online Access: | http://dx.doi.org/10.1063/5.0027022 |
| _version_ | 1818723959130030080 |
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| author | Weitao Wang D. Sebastian Arias Markus Deserno Xi Ren Rebecca E. Taylor |
| author_facet | Weitao Wang D. Sebastian Arias Markus Deserno Xi Ren Rebecca E. Taylor |
| author_sort | Weitao Wang |
| collection | DOAJ |
| description | DNA nanotechnology has proven exceptionally apt at probing and manipulating biological environments as it can create nanostructures of almost arbitrary shape that permit countless types of modifications, all while being inherently biocompatible. Emergent areas of particular interest are applications involving cellular membranes, but to fully explore the range of possibilities requires interdisciplinary knowledge of DNA nanotechnology, cell and membrane biology, and biophysics. In this review, we aim for a concise introduction to the intersection of these three fields. After briefly revisiting DNA nanotechnology, as well as the biological and mechanical properties of lipid bilayers and cellular membranes, we summarize strategies to mediate interactions between membranes and DNA nanostructures, with a focus on programmed delivery onto, into, and through lipid membranes. We also highlight emerging applications, including membrane sculpting, multicell self-assembly, spatial arrangement and organization of ligands and proteins, biomechanical sensing, synthetic DNA nanopores, biological imaging, and biomelecular sensing. Many critical but exciting challenges lie ahead, and we outline what strikes us as promising directions when translating DNA nanostructures for future in vitro and in vivo membrane applications. |
| first_indexed | 2024-12-17T21:18:48Z |
| format | Article |
| id | doaj.art-c5afd5a949a24f29acbc6e654af3434a |
| institution | Directory Open Access Journal |
| issn | 2473-2877 |
| language | English |
| last_indexed | 2024-12-17T21:18:48Z |
| publishDate | 2020-12-01 |
| publisher | AIP Publishing LLC |
| record_format | Article |
| series | APL Bioengineering |
| spelling | doaj.art-c5afd5a949a24f29acbc6e654af3434a2022-12-21T21:32:16ZengAIP Publishing LLCAPL Bioengineering2473-28772020-12-0144041507041507-1910.1063/5.0027022Emerging applications at the interface of DNA nanotechnology and cellular membranes: Perspectives from biology, engineering, and physicsWeitao Wang0D. Sebastian Arias1Markus Deserno2Xi Ren3Rebecca E. Taylor4 Department of Mechanical Engineering, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA Department of Mechanical Engineering, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA Department of Physics, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA Department of Mechanical Engineering, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USADNA nanotechnology has proven exceptionally apt at probing and manipulating biological environments as it can create nanostructures of almost arbitrary shape that permit countless types of modifications, all while being inherently biocompatible. Emergent areas of particular interest are applications involving cellular membranes, but to fully explore the range of possibilities requires interdisciplinary knowledge of DNA nanotechnology, cell and membrane biology, and biophysics. In this review, we aim for a concise introduction to the intersection of these three fields. After briefly revisiting DNA nanotechnology, as well as the biological and mechanical properties of lipid bilayers and cellular membranes, we summarize strategies to mediate interactions between membranes and DNA nanostructures, with a focus on programmed delivery onto, into, and through lipid membranes. We also highlight emerging applications, including membrane sculpting, multicell self-assembly, spatial arrangement and organization of ligands and proteins, biomechanical sensing, synthetic DNA nanopores, biological imaging, and biomelecular sensing. Many critical but exciting challenges lie ahead, and we outline what strikes us as promising directions when translating DNA nanostructures for future in vitro and in vivo membrane applications.http://dx.doi.org/10.1063/5.0027022 |
| spellingShingle | Weitao Wang D. Sebastian Arias Markus Deserno Xi Ren Rebecca E. Taylor Emerging applications at the interface of DNA nanotechnology and cellular membranes: Perspectives from biology, engineering, and physics APL Bioengineering |
| title | Emerging applications at the interface of DNA nanotechnology and cellular membranes: Perspectives from biology, engineering, and physics |
| title_full | Emerging applications at the interface of DNA nanotechnology and cellular membranes: Perspectives from biology, engineering, and physics |
| title_fullStr | Emerging applications at the interface of DNA nanotechnology and cellular membranes: Perspectives from biology, engineering, and physics |
| title_full_unstemmed | Emerging applications at the interface of DNA nanotechnology and cellular membranes: Perspectives from biology, engineering, and physics |
| title_short | Emerging applications at the interface of DNA nanotechnology and cellular membranes: Perspectives from biology, engineering, and physics |
| title_sort | emerging applications at the interface of dna nanotechnology and cellular membranes perspectives from biology engineering and physics |
| url | http://dx.doi.org/10.1063/5.0027022 |
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