Transfer and Inactivation of <i>Listeria monocytogenes</i> during Pilot-Scale Dicing and Flume Washing of Onions
This study assessed the extent of <i>L. monocytogenes</i> transfer from onions to the surface of a commercial dicer, from inoculated onions to uninoculated onions, and the efficacy of various sanitizers during the subsequent flume washing of diced onions. Spanish yellow onions (<i>...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-02-01
|
| Series: | Applied Microbiology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2673-8007/4/1/30 |
| _version_ | 1797242235509538816 |
|---|---|
| author | Andrew M. Scollon Haiqiang Wang Elliot T. Ryser |
| author_facet | Andrew M. Scollon Haiqiang Wang Elliot T. Ryser |
| author_sort | Andrew M. Scollon |
| collection | DOAJ |
| description | This study assessed the extent of <i>L. monocytogenes</i> transfer from onions to the surface of a commercial dicer, from inoculated onions to uninoculated onions, and the efficacy of various sanitizers during the subsequent flume washing of diced onions. Spanish yellow onions (<i>Allium cepa</i> L.) were dip-inoculated in a 3-strain avirulent <i>L. monocytogenes</i> cocktail (5.9 or 4.2 log CFU/50 g) and air-dried. After dicing one 2.2 kg batch of onions inoculated at ~5.9 log CFU/50 g followed by ten uninoculated batches of 2.2 kg each, <i>L. monocytogenes</i> progressively decreased from 4.6 to 2.6 log CFU/50 g in baches 1 through 10, respectively. After onions inoculated at ~4.0 log CFU/g were diced and flume washed for 2 min in tap water, electrolyzed water containing 55 ppm free chlorine, 80 ppm free chlorine from a commercial sanitizer, or 80 ppm peroxyacetic acid and dewatered on a mechanical shaker table, <i>L. monocytogenes</i> populations decreased 0.4, 0.3, 1.4, and 1.0 log, respectively, with populations of ~1.2 log CFU/mL in water for all three sanitizers. These findings should be useful in future risk assessments and aid in the development of improved industry guidelines to better enhance the safety of diced onions. |
| first_indexed | 2024-04-24T18:36:00Z |
| format | Article |
| id | doaj.art-c5b1542d0bd84decac89216d028843dc |
| institution | Directory Open Access Journal |
| issn | 2673-8007 |
| language | English |
| last_indexed | 2024-04-24T18:36:00Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Microbiology |
| spelling | doaj.art-c5b1542d0bd84decac89216d028843dc2024-03-27T13:18:59ZengMDPI AGApplied Microbiology2673-80072024-02-014143945210.3390/applmicrobiol4010030Transfer and Inactivation of <i>Listeria monocytogenes</i> during Pilot-Scale Dicing and Flume Washing of OnionsAndrew M. Scollon0Haiqiang Wang1Elliot T. Ryser2Department of Food Science and Human Nutrition, Michigan State University, East Lansing, MI 48824, USADepartment of Food Science and Human Nutrition, Michigan State University, East Lansing, MI 48824, USADepartment of Food Science and Human Nutrition, Michigan State University, East Lansing, MI 48824, USAThis study assessed the extent of <i>L. monocytogenes</i> transfer from onions to the surface of a commercial dicer, from inoculated onions to uninoculated onions, and the efficacy of various sanitizers during the subsequent flume washing of diced onions. Spanish yellow onions (<i>Allium cepa</i> L.) were dip-inoculated in a 3-strain avirulent <i>L. monocytogenes</i> cocktail (5.9 or 4.2 log CFU/50 g) and air-dried. After dicing one 2.2 kg batch of onions inoculated at ~5.9 log CFU/50 g followed by ten uninoculated batches of 2.2 kg each, <i>L. monocytogenes</i> progressively decreased from 4.6 to 2.6 log CFU/50 g in baches 1 through 10, respectively. After onions inoculated at ~4.0 log CFU/g were diced and flume washed for 2 min in tap water, electrolyzed water containing 55 ppm free chlorine, 80 ppm free chlorine from a commercial sanitizer, or 80 ppm peroxyacetic acid and dewatered on a mechanical shaker table, <i>L. monocytogenes</i> populations decreased 0.4, 0.3, 1.4, and 1.0 log, respectively, with populations of ~1.2 log CFU/mL in water for all three sanitizers. These findings should be useful in future risk assessments and aid in the development of improved industry guidelines to better enhance the safety of diced onions.https://www.mdpi.com/2673-8007/4/1/30<i>Listeria monocytogenes</i>onionscross-contaminationdicingchlorineelectrolyzed water |
| spellingShingle | Andrew M. Scollon Haiqiang Wang Elliot T. Ryser Transfer and Inactivation of <i>Listeria monocytogenes</i> during Pilot-Scale Dicing and Flume Washing of Onions Applied Microbiology <i>Listeria monocytogenes</i> onions cross-contamination dicing chlorine electrolyzed water |
| title | Transfer and Inactivation of <i>Listeria monocytogenes</i> during Pilot-Scale Dicing and Flume Washing of Onions |
| title_full | Transfer and Inactivation of <i>Listeria monocytogenes</i> during Pilot-Scale Dicing and Flume Washing of Onions |
| title_fullStr | Transfer and Inactivation of <i>Listeria monocytogenes</i> during Pilot-Scale Dicing and Flume Washing of Onions |
| title_full_unstemmed | Transfer and Inactivation of <i>Listeria monocytogenes</i> during Pilot-Scale Dicing and Flume Washing of Onions |
| title_short | Transfer and Inactivation of <i>Listeria monocytogenes</i> during Pilot-Scale Dicing and Flume Washing of Onions |
| title_sort | transfer and inactivation of i listeria monocytogenes i during pilot scale dicing and flume washing of onions |
| topic | <i>Listeria monocytogenes</i> onions cross-contamination dicing chlorine electrolyzed water |
| url | https://www.mdpi.com/2673-8007/4/1/30 |
| work_keys_str_mv | AT andrewmscollon transferandinactivationofilisteriamonocytogenesiduringpilotscaledicingandflumewashingofonions AT haiqiangwang transferandinactivationofilisteriamonocytogenesiduringpilotscaledicingandflumewashingofonions AT elliottryser transferandinactivationofilisteriamonocytogenesiduringpilotscaledicingandflumewashingofonions |