Homeology of sex chromosomes in Amazonian Harttia armored catfishes supports the X-fission hypothesis for the X1X2Y sex chromosome system origin
Abstract The Neotropical monophyletic catfish genus Harttia represents an excellent model to study karyotype and sex chromosome evolution in teleosts. Its species split into three phylogenetic clades distributed along the Brazilian territory and they differ widely in karyotype traits, including the...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-42617-w |
| _version_ | 1797559790711341056 |
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| author | Francisco de Menezes Cavalcante Sassi Alexandr Sember Geize Aparecida Deon Thomas Liehr Niklas Padutsch Osvaldo Takeshi Oyakawa Marcelo Ricardo Vicari Luiz Antonio Carlos Bertollo Orlando Moreira-Filho Marcelo de Bello Cioffi |
| author_facet | Francisco de Menezes Cavalcante Sassi Alexandr Sember Geize Aparecida Deon Thomas Liehr Niklas Padutsch Osvaldo Takeshi Oyakawa Marcelo Ricardo Vicari Luiz Antonio Carlos Bertollo Orlando Moreira-Filho Marcelo de Bello Cioffi |
| author_sort | Francisco de Menezes Cavalcante Sassi |
| collection | DOAJ |
| description | Abstract The Neotropical monophyletic catfish genus Harttia represents an excellent model to study karyotype and sex chromosome evolution in teleosts. Its species split into three phylogenetic clades distributed along the Brazilian territory and they differ widely in karyotype traits, including the presence of standard or multiple sex chromosome systems in some members. Here, we investigate the chromosomal rearrangements and associated synteny blocks involved in the origin of a multiple X1X2Y sex chromosome system present in three out of six sampled Amazonian-clade species. Using 5S and 18S ribosomal DNA fluorescence in situ hybridization and whole chromosome painting with probes corresponding to X1 and X2 chromosomes of X1X2Y system from H. punctata, we confirm previous assumptions that X1X2Y sex chromosome systems of H. punctata, H. duriventris and H. villasboas represent the same linkage groups which also form the putative XY sex chromosomes of H. rondoni. The shared homeology between X1X2Y sex chromosomes suggests they might have originated once in the common ancestor of these closely related species. A joint arrangement of mapped H. punctata X1 and X2 sex chromosomes in early diverging species of different Harttia clades suggests that the X1X2Y sex chromosome system may have formed through an X chromosome fission rather than previously proposed Y-autosome fusion. |
| first_indexed | 2024-03-10T17:50:15Z |
| format | Article |
| id | doaj.art-c5b345a0caa2441fab61318b09ccbc0c |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-10T17:50:15Z |
| publishDate | 2023-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-c5b345a0caa2441fab61318b09ccbc0c2023-11-20T09:23:46ZengNature PortfolioScientific Reports2045-23222023-09-0113111110.1038/s41598-023-42617-wHomeology of sex chromosomes in Amazonian Harttia armored catfishes supports the X-fission hypothesis for the X1X2Y sex chromosome system originFrancisco de Menezes Cavalcante Sassi0Alexandr Sember1Geize Aparecida Deon2Thomas Liehr3Niklas Padutsch4Osvaldo Takeshi Oyakawa5Marcelo Ricardo Vicari6Luiz Antonio Carlos Bertollo7Orlando Moreira-Filho8Marcelo de Bello Cioffi9Laboratório de Citogenética de Peixes, Departamento de Genética e Evolução, Universidade Federal de São CarlosLaboratory of Fish Genetics, Institute of Animal Physiology and Genetics, Czech Academy of SciencesLaboratório de Citogenética de Peixes, Departamento de Genética e Evolução, Universidade Federal de São CarlosInstitut für Humangenetik, Universitätsklinikum JenaInstitut für Humangenetik, Universitätsklinikum JenaMuseu de Zoologia, Universidade de São PauloDepartamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta GrossaLaboratório de Citogenética de Peixes, Departamento de Genética e Evolução, Universidade Federal de São CarlosLaboratório de Citogenética de Peixes, Departamento de Genética e Evolução, Universidade Federal de São CarlosLaboratório de Citogenética de Peixes, Departamento de Genética e Evolução, Universidade Federal de São CarlosAbstract The Neotropical monophyletic catfish genus Harttia represents an excellent model to study karyotype and sex chromosome evolution in teleosts. Its species split into three phylogenetic clades distributed along the Brazilian territory and they differ widely in karyotype traits, including the presence of standard or multiple sex chromosome systems in some members. Here, we investigate the chromosomal rearrangements and associated synteny blocks involved in the origin of a multiple X1X2Y sex chromosome system present in three out of six sampled Amazonian-clade species. Using 5S and 18S ribosomal DNA fluorescence in situ hybridization and whole chromosome painting with probes corresponding to X1 and X2 chromosomes of X1X2Y system from H. punctata, we confirm previous assumptions that X1X2Y sex chromosome systems of H. punctata, H. duriventris and H. villasboas represent the same linkage groups which also form the putative XY sex chromosomes of H. rondoni. The shared homeology between X1X2Y sex chromosomes suggests they might have originated once in the common ancestor of these closely related species. A joint arrangement of mapped H. punctata X1 and X2 sex chromosomes in early diverging species of different Harttia clades suggests that the X1X2Y sex chromosome system may have formed through an X chromosome fission rather than previously proposed Y-autosome fusion.https://doi.org/10.1038/s41598-023-42617-w |
| spellingShingle | Francisco de Menezes Cavalcante Sassi Alexandr Sember Geize Aparecida Deon Thomas Liehr Niklas Padutsch Osvaldo Takeshi Oyakawa Marcelo Ricardo Vicari Luiz Antonio Carlos Bertollo Orlando Moreira-Filho Marcelo de Bello Cioffi Homeology of sex chromosomes in Amazonian Harttia armored catfishes supports the X-fission hypothesis for the X1X2Y sex chromosome system origin Scientific Reports |
| title | Homeology of sex chromosomes in Amazonian Harttia armored catfishes supports the X-fission hypothesis for the X1X2Y sex chromosome system origin |
| title_full | Homeology of sex chromosomes in Amazonian Harttia armored catfishes supports the X-fission hypothesis for the X1X2Y sex chromosome system origin |
| title_fullStr | Homeology of sex chromosomes in Amazonian Harttia armored catfishes supports the X-fission hypothesis for the X1X2Y sex chromosome system origin |
| title_full_unstemmed | Homeology of sex chromosomes in Amazonian Harttia armored catfishes supports the X-fission hypothesis for the X1X2Y sex chromosome system origin |
| title_short | Homeology of sex chromosomes in Amazonian Harttia armored catfishes supports the X-fission hypothesis for the X1X2Y sex chromosome system origin |
| title_sort | homeology of sex chromosomes in amazonian harttia armored catfishes supports the x fission hypothesis for the x1x2y sex chromosome system origin |
| url | https://doi.org/10.1038/s41598-023-42617-w |
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