Identification of serotonergic (5HT1A-type) receptors in broiler small intestine by application of serotonin and its agonists and antagonists
Serotonin or 5-hydroxytryptamine (5-HT), is a monoamine neurotransmitter synthesised from L-tryptophan in serotonergic neurons and enterochromaffin cells of the gastrointestinal tract. This neurotransmitter is widely distributed in the animal and plant kingdom and regulates some central and...
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| Format: | Article |
| Language: | English |
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Faculty of Veterinary Medicine, Belgrade
2011-01-01
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| Series: | Veterinarski Glasnik |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0350-2457/2011/0350-24571102051M.pdf |
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| author | Mujezinović Indira Ćupić Vitomir Smajlović Ahmed Muminović Mehmed |
| author_facet | Mujezinović Indira Ćupić Vitomir Smajlović Ahmed Muminović Mehmed |
| author_sort | Mujezinović Indira |
| collection | DOAJ |
| description | Serotonin or 5-hydroxytryptamine (5-HT), is a monoamine neurotransmitter
synthesised from L-tryptophan in serotonergic neurons and enterochromaffin
cells of the gastrointestinal tract. This neurotransmitter is widely
distributed in the animal and plant kingdom and regulates some central and
peripheral functions through several types of specific serotonergic (5-HT)
receptors. Since it is known that the effect of serotonin, especially in
pathological conditions, is very important, we believe that determining the
types of receptors for this substance would make it possible to use their
agonist or antagonists, which would undoubtedly enhance the pharmacotherapy
of functional disruption of the small intestine in broilers. Investigations
were carried out on isolated smooth muscle strips of the circular and
longitudinal layer of the broiler small intestine (strip dimension 3-4 mm x 2
cm). The muscle strips were placed in an isolated organ bath. The mechanical
activity of the preparations was recorded via an isotonic force transducer
coupled to a pen recorder. This was done following the addition of serotonin
(nonselective 5-HT agonist), 8-OH-DPAT (selective 5-HT1A agonist) and
spiroxatrin (selective 5-HT1A antagonist). The sensitivity of the tissues to
acetylcholine was tested before starting the experiments. Using the obtained
results, it can be concluded that 5HT1A type receptors are present in smooth
muscles of the broiler small intestine, duodenum and ileum, especially in the
longitudinal smooth muscle layer which reacted with contractions even to low
serotonin concentration (10-6), but not in the jejunum. |
| first_indexed | 2024-12-20T15:34:12Z |
| format | Article |
| id | doaj.art-c5b5d383afac473091120c7b8b0dcdde |
| institution | Directory Open Access Journal |
| issn | 0350-2457 2406-0771 |
| language | English |
| last_indexed | 2024-12-20T15:34:12Z |
| publishDate | 2011-01-01 |
| publisher | Faculty of Veterinary Medicine, Belgrade |
| record_format | Article |
| series | Veterinarski Glasnik |
| spelling | doaj.art-c5b5d383afac473091120c7b8b0dcdde2022-12-21T19:35:29ZengFaculty of Veterinary Medicine, BelgradeVeterinarski Glasnik0350-24572406-07712011-01-01651-2515910.2298/VETGL1102051M0350-24571102051MIdentification of serotonergic (5HT1A-type) receptors in broiler small intestine by application of serotonin and its agonists and antagonistsMujezinović Indira0Ćupić Vitomir1Smajlović Ahmed2Muminović Mehmed3Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Sarajevo, Bosnia and HerzegovinaDepartment of Pharmacology and Toxicology, Faculty of Veterinary Medicine, BelgradeDepartment of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Sarajevo, Bosnia and HerzegovinaDepartment of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Sarajevo, Bosnia and HerzegovinaSerotonin or 5-hydroxytryptamine (5-HT), is a monoamine neurotransmitter synthesised from L-tryptophan in serotonergic neurons and enterochromaffin cells of the gastrointestinal tract. This neurotransmitter is widely distributed in the animal and plant kingdom and regulates some central and peripheral functions through several types of specific serotonergic (5-HT) receptors. Since it is known that the effect of serotonin, especially in pathological conditions, is very important, we believe that determining the types of receptors for this substance would make it possible to use their agonist or antagonists, which would undoubtedly enhance the pharmacotherapy of functional disruption of the small intestine in broilers. Investigations were carried out on isolated smooth muscle strips of the circular and longitudinal layer of the broiler small intestine (strip dimension 3-4 mm x 2 cm). The muscle strips were placed in an isolated organ bath. The mechanical activity of the preparations was recorded via an isotonic force transducer coupled to a pen recorder. This was done following the addition of serotonin (nonselective 5-HT agonist), 8-OH-DPAT (selective 5-HT1A agonist) and spiroxatrin (selective 5-HT1A antagonist). The sensitivity of the tissues to acetylcholine was tested before starting the experiments. Using the obtained results, it can be concluded that 5HT1A type receptors are present in smooth muscles of the broiler small intestine, duodenum and ileum, especially in the longitudinal smooth muscle layer which reacted with contractions even to low serotonin concentration (10-6), but not in the jejunum.http://www.doiserbia.nb.rs/img/doi/0350-2457/2011/0350-24571102051M.pdfbroilersserotoninserotonergic receptorsisolated smooth musclesmall intestine |
| spellingShingle | Mujezinović Indira Ćupić Vitomir Smajlović Ahmed Muminović Mehmed Identification of serotonergic (5HT1A-type) receptors in broiler small intestine by application of serotonin and its agonists and antagonists Veterinarski Glasnik broilers serotonin serotonergic receptors isolated smooth muscle small intestine |
| title | Identification of serotonergic (5HT1A-type) receptors in broiler small intestine by application of serotonin and its agonists and antagonists |
| title_full | Identification of serotonergic (5HT1A-type) receptors in broiler small intestine by application of serotonin and its agonists and antagonists |
| title_fullStr | Identification of serotonergic (5HT1A-type) receptors in broiler small intestine by application of serotonin and its agonists and antagonists |
| title_full_unstemmed | Identification of serotonergic (5HT1A-type) receptors in broiler small intestine by application of serotonin and its agonists and antagonists |
| title_short | Identification of serotonergic (5HT1A-type) receptors in broiler small intestine by application of serotonin and its agonists and antagonists |
| title_sort | identification of serotonergic 5ht1a type receptors in broiler small intestine by application of serotonin and its agonists and antagonists |
| topic | broilers serotonin serotonergic receptors isolated smooth muscle small intestine |
| url | http://www.doiserbia.nb.rs/img/doi/0350-2457/2011/0350-24571102051M.pdf |
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