Development of Stable Liposomal Drug Delivery System of Thymoquinone and Its In Vitro Anticancer Studies Using Breast Cancer and Cervical Cancer Cell Lines
Thymoquinone, a well-known phytoconstituent derived from the seeds of <i>Nigella sativa</i>, exhibits unique pharmacological activities However, despite the various medicinal properties of thymoquinone, its administration in vivo remains challenging due to poor aqueous solubility, bioava...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/19/6744 |
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| author | Mohammad Hossain Shariare Md Asaduzzaman Khan Abdullah Al-Masum Junayet Hossain Khan Jamal Uddin Mohsin Kazi |
| author_facet | Mohammad Hossain Shariare Md Asaduzzaman Khan Abdullah Al-Masum Junayet Hossain Khan Jamal Uddin Mohsin Kazi |
| author_sort | Mohammad Hossain Shariare |
| collection | DOAJ |
| description | Thymoquinone, a well-known phytoconstituent derived from the seeds of <i>Nigella sativa</i>, exhibits unique pharmacological activities However, despite the various medicinal properties of thymoquinone, its administration in vivo remains challenging due to poor aqueous solubility, bioavailability, and stability. Therefore, an advanced drugdelivery system is required to improve the therapeutic outcome of thymoquinone by enhancing its solubility and stability in biological systems. Therefore, this study is mainly focused on preparing thymoquinone-loaded liposomes to improve its physicochemical stability in gastric media and its performance in different cancer cell line studies. Liposomes were prepared using phospholipid extracted from egg yolk. The liposomal nano preparations were evaluated in terms of hydrodynamic diameter, zeta potential, microscopic analysis, and entrapment efficiency. Cell-viability measurements were conducted using breast and cervical cancer cell lines. Optimized liposomal preparation exhibited polygonal, globule-like shape with a hydrodynamic diameter of less than 260 nm, PDI of 0.6, and zeta potential values of −23.0 mV. Solid-state characterizations performed using DSC and XRPD showed that the freeze-dried liposomal preparations were amorphous in nature. Gastric pH stability data showed no physical changes (precipitation, degradation) or significant growth in the average size of blank and thymoquinone-loaded liposomes after 24 h. Cell line studies exhibited better performance for thymoquinone-loaded liposomal drug delivery system compared with the thymoquinone-only solution; this finding can play a critical role in improving breast and cervical cancer treatment management. |
| first_indexed | 2024-03-09T21:23:06Z |
| format | Article |
| id | doaj.art-c5b5dd3d075e4f61af0d496dcb48dbcb |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T21:23:06Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-c5b5dd3d075e4f61af0d496dcb48dbcb2023-11-23T21:16:43ZengMDPI AGMolecules1420-30492022-10-012719674410.3390/molecules27196744Development of Stable Liposomal Drug Delivery System of Thymoquinone and Its In Vitro Anticancer Studies Using Breast Cancer and Cervical Cancer Cell LinesMohammad Hossain Shariare0Md Asaduzzaman Khan1Abdullah Al-Masum2Junayet Hossain Khan3Jamal Uddin4Mohsin Kazi5Department of Pharmaceutical Sciences, North South University, Dhaka 1229, BangladeshResearch Center for Preclinical Medicine, Southwest Medical University, Luzhou 646000, ChinaDepartment of Pharmaceutical Sciences, North South University, Dhaka 1229, BangladeshDepartment of Pharmaceutical Sciences, North South University, Dhaka 1229, BangladeshCenter for Nanotechnology, Department of Natural Sciences, Coppin State University, Baltimore, MD 21216, USADepartment of Pharmaceutics, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi ArabiaThymoquinone, a well-known phytoconstituent derived from the seeds of <i>Nigella sativa</i>, exhibits unique pharmacological activities However, despite the various medicinal properties of thymoquinone, its administration in vivo remains challenging due to poor aqueous solubility, bioavailability, and stability. Therefore, an advanced drugdelivery system is required to improve the therapeutic outcome of thymoquinone by enhancing its solubility and stability in biological systems. Therefore, this study is mainly focused on preparing thymoquinone-loaded liposomes to improve its physicochemical stability in gastric media and its performance in different cancer cell line studies. Liposomes were prepared using phospholipid extracted from egg yolk. The liposomal nano preparations were evaluated in terms of hydrodynamic diameter, zeta potential, microscopic analysis, and entrapment efficiency. Cell-viability measurements were conducted using breast and cervical cancer cell lines. Optimized liposomal preparation exhibited polygonal, globule-like shape with a hydrodynamic diameter of less than 260 nm, PDI of 0.6, and zeta potential values of −23.0 mV. Solid-state characterizations performed using DSC and XRPD showed that the freeze-dried liposomal preparations were amorphous in nature. Gastric pH stability data showed no physical changes (precipitation, degradation) or significant growth in the average size of blank and thymoquinone-loaded liposomes after 24 h. Cell line studies exhibited better performance for thymoquinone-loaded liposomal drug delivery system compared with the thymoquinone-only solution; this finding can play a critical role in improving breast and cervical cancer treatment management.https://www.mdpi.com/1420-3049/27/19/6744liposomethymoquinonecell cytotoxicityparticle sizeanticancer |
| spellingShingle | Mohammad Hossain Shariare Md Asaduzzaman Khan Abdullah Al-Masum Junayet Hossain Khan Jamal Uddin Mohsin Kazi Development of Stable Liposomal Drug Delivery System of Thymoquinone and Its In Vitro Anticancer Studies Using Breast Cancer and Cervical Cancer Cell Lines Molecules liposome thymoquinone cell cytotoxicity particle size anticancer |
| title | Development of Stable Liposomal Drug Delivery System of Thymoquinone and Its In Vitro Anticancer Studies Using Breast Cancer and Cervical Cancer Cell Lines |
| title_full | Development of Stable Liposomal Drug Delivery System of Thymoquinone and Its In Vitro Anticancer Studies Using Breast Cancer and Cervical Cancer Cell Lines |
| title_fullStr | Development of Stable Liposomal Drug Delivery System of Thymoquinone and Its In Vitro Anticancer Studies Using Breast Cancer and Cervical Cancer Cell Lines |
| title_full_unstemmed | Development of Stable Liposomal Drug Delivery System of Thymoquinone and Its In Vitro Anticancer Studies Using Breast Cancer and Cervical Cancer Cell Lines |
| title_short | Development of Stable Liposomal Drug Delivery System of Thymoquinone and Its In Vitro Anticancer Studies Using Breast Cancer and Cervical Cancer Cell Lines |
| title_sort | development of stable liposomal drug delivery system of thymoquinone and its in vitro anticancer studies using breast cancer and cervical cancer cell lines |
| topic | liposome thymoquinone cell cytotoxicity particle size anticancer |
| url | https://www.mdpi.com/1420-3049/27/19/6744 |
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