Nickel ions influence the transepithelial sodium transport in the trachea, intestine and skin
Abstract Measurements of transepithelial potential and resistance in tissue and organ model systems enable the evaluation of the Ni2+ effect on the epithelial sodium channels, aquaporin 3, and the sodium–potassium pump in the epithelial cells. The aim of the presented study was to assess the immedia...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-04-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-33690-2 |
| _version_ | 1797836438458335232 |
|---|---|
| author | Iga Hołyńska-Iwan Marta Sobiesiak Wojciech Kowalczyk Marcin Wróblewski Anna Cwynar Karolina Szewczyk-Golec |
| author_facet | Iga Hołyńska-Iwan Marta Sobiesiak Wojciech Kowalczyk Marcin Wróblewski Anna Cwynar Karolina Szewczyk-Golec |
| author_sort | Iga Hołyńska-Iwan |
| collection | DOAJ |
| description | Abstract Measurements of transepithelial potential and resistance in tissue and organ model systems enable the evaluation of the Ni2+ effect on the epithelial sodium channels, aquaporin 3, and the sodium–potassium pump in the epithelial cells. The aim of the presented study was to assess the immediate and prolonged effect of nickel ions on the transport of sodium ions in tissues exposed to direct contact with nickel, including airways, digestive tract and the skin. The influence of 0.1 mM nickel solution was performed on the trachea (n = 34), intestine (n = 44), and skin (n = 51) samples descended from 16 New Zealand albino rabbits. The electrophysiological parameters were measured in a modified Ussing chamber in stationary conditions and during a 15-s mechanical-chemical stimulation. A statistically significant decrease in the electric resistance values and the smallest range of the measured potential were observed for the Ni-treated trachea specimens. The use of nickel solution did not affect the sodium transport in the intestine epithelium. The skin fragments showed altered sodium ion transport, as demonstrated by the lower range and intensity of the measured potential. The gastrointestinal tract seems to be an organ best adapted to contact with nickel ions. In airways, nickel ions most likely enter epithelial cells and the space between them, modifying proteins and the airway surface liquid. The skin turned out to be the most sensitive tissue to the intensification of sodium ion transport through nickel ions. |
| first_indexed | 2024-04-09T15:10:03Z |
| format | Article |
| id | doaj.art-c5bbf26ff2874bc4a3e4eebfb433c40a |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T15:10:03Z |
| publishDate | 2023-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-c5bbf26ff2874bc4a3e4eebfb433c40a2023-04-30T11:17:25ZengNature PortfolioScientific Reports2045-23222023-04-0113111110.1038/s41598-023-33690-2Nickel ions influence the transepithelial sodium transport in the trachea, intestine and skinIga Hołyńska-Iwan0Marta Sobiesiak1Wojciech Kowalczyk2Marcin Wróblewski3Anna Cwynar4Karolina Szewczyk-Golec5Laboratory of Electrophysiology of Epithelial Tissue and Skin, Department of Pathobiochemistry and Clinical Chemistry, Faculty of Pharmacy, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in TorunDepartment of Inorganic and Analytical Chemistry, Faculty of Pharmacy, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in TorunClinic of Allergology, Clinical Immunology and Internal Diseases, Dr Jan Biziel’s University Hospital No. 2Department of Medical Biology and Biochemistry, Faculty of Medicine, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in TorunLaboratory of Electrophysiology of Epithelial Tissue and Skin, Department of Pathobiochemistry and Clinical Chemistry, Faculty of Pharmacy, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in TorunDepartment of Medical Biology and Biochemistry, Faculty of Medicine, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in TorunAbstract Measurements of transepithelial potential and resistance in tissue and organ model systems enable the evaluation of the Ni2+ effect on the epithelial sodium channels, aquaporin 3, and the sodium–potassium pump in the epithelial cells. The aim of the presented study was to assess the immediate and prolonged effect of nickel ions on the transport of sodium ions in tissues exposed to direct contact with nickel, including airways, digestive tract and the skin. The influence of 0.1 mM nickel solution was performed on the trachea (n = 34), intestine (n = 44), and skin (n = 51) samples descended from 16 New Zealand albino rabbits. The electrophysiological parameters were measured in a modified Ussing chamber in stationary conditions and during a 15-s mechanical-chemical stimulation. A statistically significant decrease in the electric resistance values and the smallest range of the measured potential were observed for the Ni-treated trachea specimens. The use of nickel solution did not affect the sodium transport in the intestine epithelium. The skin fragments showed altered sodium ion transport, as demonstrated by the lower range and intensity of the measured potential. The gastrointestinal tract seems to be an organ best adapted to contact with nickel ions. In airways, nickel ions most likely enter epithelial cells and the space between them, modifying proteins and the airway surface liquid. The skin turned out to be the most sensitive tissue to the intensification of sodium ion transport through nickel ions.https://doi.org/10.1038/s41598-023-33690-2 |
| spellingShingle | Iga Hołyńska-Iwan Marta Sobiesiak Wojciech Kowalczyk Marcin Wróblewski Anna Cwynar Karolina Szewczyk-Golec Nickel ions influence the transepithelial sodium transport in the trachea, intestine and skin Scientific Reports |
| title | Nickel ions influence the transepithelial sodium transport in the trachea, intestine and skin |
| title_full | Nickel ions influence the transepithelial sodium transport in the trachea, intestine and skin |
| title_fullStr | Nickel ions influence the transepithelial sodium transport in the trachea, intestine and skin |
| title_full_unstemmed | Nickel ions influence the transepithelial sodium transport in the trachea, intestine and skin |
| title_short | Nickel ions influence the transepithelial sodium transport in the trachea, intestine and skin |
| title_sort | nickel ions influence the transepithelial sodium transport in the trachea intestine and skin |
| url | https://doi.org/10.1038/s41598-023-33690-2 |
| work_keys_str_mv | AT igahołynskaiwan nickelionsinfluencethetransepithelialsodiumtransportinthetracheaintestineandskin AT martasobiesiak nickelionsinfluencethetransepithelialsodiumtransportinthetracheaintestineandskin AT wojciechkowalczyk nickelionsinfluencethetransepithelialsodiumtransportinthetracheaintestineandskin AT marcinwroblewski nickelionsinfluencethetransepithelialsodiumtransportinthetracheaintestineandskin AT annacwynar nickelionsinfluencethetransepithelialsodiumtransportinthetracheaintestineandskin AT karolinaszewczykgolec nickelionsinfluencethetransepithelialsodiumtransportinthetracheaintestineandskin |