Effect of dietary zinc source, zinc concentration, and exogenous phytase on intestinal phytate degradation products, bone mineralization, and zinc status of broiler chickens

ABSTRACT: This study aimed to determine the effect of Zn source and dietary level on intestinal myo-inositol hexakisphosphate (InsP6) disappearance, intestinal accumulation of lower InsP and myo-inositol (MI), prececal mineral digestibility, bone mineralization, and Zn status of broilers without and...

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Main Authors: Hanna Philippi, Vera Sommerfeld, Oluyinka A. Olukosi, Wilhelm Windisch, Alessandra Monteiro, Markus Rodehutscord
Format: Article
Language:English
Published: Elsevier 2023-12-01
Series:Poultry Science
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S003257912300679X
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author Hanna Philippi
Vera Sommerfeld
Oluyinka A. Olukosi
Wilhelm Windisch
Alessandra Monteiro
Markus Rodehutscord
author_facet Hanna Philippi
Vera Sommerfeld
Oluyinka A. Olukosi
Wilhelm Windisch
Alessandra Monteiro
Markus Rodehutscord
author_sort Hanna Philippi
collection DOAJ
description ABSTRACT: This study aimed to determine the effect of Zn source and dietary level on intestinal myo-inositol hexakisphosphate (InsP6) disappearance, intestinal accumulation of lower InsP and myo-inositol (MI), prececal mineral digestibility, bone mineralization, and Zn status of broilers without and with exogenous phytase in the feed. Male Ross 308 broilers were allocated in groups of 10 to 8 treatments with 8 pens each. Experimental diets were fed from d 7 to d 28 and contained 33 mg/kg dry matter plant-intrinsic Zn. Experimental factors were phytase supplementation (0 or 750 FTU/kg) and Zn source (none [0 mg/kg Zn], Zn-sulfate [30 mg/kg Zn], Zn-oxide [30 mg/kg Zn]). Additional treatments with 90 mg/kg Zn as Zn-sulfate or Zn-oxide and phytase were included to test the effect of Zn level. No Zn source or Zn level effects were observed for ADG, feed conversion ratio, prececal P digestibility, intestinal InsP6 disappearance, and bone ash concentration. However, those measurements were increased by exogenous phytase (P < 0.001), except the feed conversion ratio, which was decreased (P < 0.001). Ileal MI concentrations were affected by phytase × Zn source interaction (P < 0.030). Birds receiving exogenous phytase and Zn supplementation had the highest MI concentrations regardless of exogenous Zn source, whereas MI concentrations were intermediate for birds receiving exogenous phytase only. Exogenous phytase and exogenous Zn source increased the Zn concentration in bone and blood of broilers (P < 0.001). In conclusion, measures of exogenous phytase efficacy were not affected by phytase × Zn source interaction. Further studies are needed to rule out an effect from Zn sources other than those tested in this study and to investigate the effect of Zn supplementation on endogenous phosphatases. The missing effect of increasing Zn supplementation from 30 to 90 mg/kg in phytase-supplemented diets gives reason to reconsider the Zn supplementation level used by the industry.
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spelling doaj.art-c5bdff3d429242eeae57e9ee50b34fa72023-12-07T05:27:25ZengElsevierPoultry Science0032-57912023-12-0110212103160Effect of dietary zinc source, zinc concentration, and exogenous phytase on intestinal phytate degradation products, bone mineralization, and zinc status of broiler chickensHanna Philippi0Vera Sommerfeld1Oluyinka A. Olukosi2Wilhelm Windisch3Alessandra Monteiro4Markus Rodehutscord5Institute of Animal Science, University of Hohenheim, Stuttgart 70599, GermanyInstitute of Animal Science, University of Hohenheim, Stuttgart 70599, GermanyDepartment of Poultry Science, University of Georgia, Athens, GA 30602, USAChair of Animal Nutrition, TUM School of Life Sciences, Technical University of Munich, Freising 85354, GermanyAnimine, Annecy 74960, FranceInstitute of Animal Science, University of Hohenheim, Stuttgart 70599, Germany; Corresponding author:ABSTRACT: This study aimed to determine the effect of Zn source and dietary level on intestinal myo-inositol hexakisphosphate (InsP6) disappearance, intestinal accumulation of lower InsP and myo-inositol (MI), prececal mineral digestibility, bone mineralization, and Zn status of broilers without and with exogenous phytase in the feed. Male Ross 308 broilers were allocated in groups of 10 to 8 treatments with 8 pens each. Experimental diets were fed from d 7 to d 28 and contained 33 mg/kg dry matter plant-intrinsic Zn. Experimental factors were phytase supplementation (0 or 750 FTU/kg) and Zn source (none [0 mg/kg Zn], Zn-sulfate [30 mg/kg Zn], Zn-oxide [30 mg/kg Zn]). Additional treatments with 90 mg/kg Zn as Zn-sulfate or Zn-oxide and phytase were included to test the effect of Zn level. No Zn source or Zn level effects were observed for ADG, feed conversion ratio, prececal P digestibility, intestinal InsP6 disappearance, and bone ash concentration. However, those measurements were increased by exogenous phytase (P < 0.001), except the feed conversion ratio, which was decreased (P < 0.001). Ileal MI concentrations were affected by phytase × Zn source interaction (P < 0.030). Birds receiving exogenous phytase and Zn supplementation had the highest MI concentrations regardless of exogenous Zn source, whereas MI concentrations were intermediate for birds receiving exogenous phytase only. Exogenous phytase and exogenous Zn source increased the Zn concentration in bone and blood of broilers (P < 0.001). In conclusion, measures of exogenous phytase efficacy were not affected by phytase × Zn source interaction. Further studies are needed to rule out an effect from Zn sources other than those tested in this study and to investigate the effect of Zn supplementation on endogenous phosphatases. The missing effect of increasing Zn supplementation from 30 to 90 mg/kg in phytase-supplemented diets gives reason to reconsider the Zn supplementation level used by the industry.http://www.sciencedirect.com/science/article/pii/S003257912300679Xphytate degradationzincphytasebone mineralizationbroiler
spellingShingle Hanna Philippi
Vera Sommerfeld
Oluyinka A. Olukosi
Wilhelm Windisch
Alessandra Monteiro
Markus Rodehutscord
Effect of dietary zinc source, zinc concentration, and exogenous phytase on intestinal phytate degradation products, bone mineralization, and zinc status of broiler chickens
Poultry Science
phytate degradation
zinc
phytase
bone mineralization
broiler
title Effect of dietary zinc source, zinc concentration, and exogenous phytase on intestinal phytate degradation products, bone mineralization, and zinc status of broiler chickens
title_full Effect of dietary zinc source, zinc concentration, and exogenous phytase on intestinal phytate degradation products, bone mineralization, and zinc status of broiler chickens
title_fullStr Effect of dietary zinc source, zinc concentration, and exogenous phytase on intestinal phytate degradation products, bone mineralization, and zinc status of broiler chickens
title_full_unstemmed Effect of dietary zinc source, zinc concentration, and exogenous phytase on intestinal phytate degradation products, bone mineralization, and zinc status of broiler chickens
title_short Effect of dietary zinc source, zinc concentration, and exogenous phytase on intestinal phytate degradation products, bone mineralization, and zinc status of broiler chickens
title_sort effect of dietary zinc source zinc concentration and exogenous phytase on intestinal phytate degradation products bone mineralization and zinc status of broiler chickens
topic phytate degradation
zinc
phytase
bone mineralization
broiler
url http://www.sciencedirect.com/science/article/pii/S003257912300679X
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