Expression of myogenes in longissimus dorsi muscle during prenatal development in commercial and local Piau pigs
Abstract This study used qRT-PCR to examine variation in the expression of 13 myogenes during muscle development in four prenatal periods (21, 40, 70 and 90 days post-insemination) in commercial (the three-way Duroc, Landrace and Large-White cross) and local Piau pig breeds that differ in muscle mas...
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Sociedade Brasileira de Genética
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| Series: | Genetics and Molecular Biology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016005017102&lng=en&tlng=en |
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| author | Evelyze Pinheiro dos Reis Débora Martins Paixão Otávio José Bernardes Brustolini Fabyano Fonseca e Silva Walmir Silva Flávio Marcos Gomes de Araújo Anna Christina de Matos Salim Guilherme Oliveira Simone Eliza Facioni Guimarães |
| author_facet | Evelyze Pinheiro dos Reis Débora Martins Paixão Otávio José Bernardes Brustolini Fabyano Fonseca e Silva Walmir Silva Flávio Marcos Gomes de Araújo Anna Christina de Matos Salim Guilherme Oliveira Simone Eliza Facioni Guimarães |
| author_sort | Evelyze Pinheiro dos Reis |
| collection | DOAJ |
| description | Abstract This study used qRT-PCR to examine variation in the expression of 13 myogenes during muscle development in four prenatal periods (21, 40, 70 and 90 days post-insemination) in commercial (the three-way Duroc, Landrace and Large-White cross) and local Piau pig breeds that differ in muscle mass. There was no variation in the expression of the CHD8, EID2B, HIF1AN, IKBKB, RSPO3, SOX7 and SUFU genes at the various prenatal ages or between breeds. The MAP2K1 and RBM24 genes showed similar expression between commercial and Piau pigs but greater expression (p < 0.05) in at least one prenatal period. Pair-wise comparisons of prenatal periods in each breed showed that only the CSRP3, LEF1, MRAS and MYOG genes had higher expression (p < 0.05) in at least one prenatal period in commercial and Piau pigs. Overall, these results identified the LEF1 gene as a primary candidate to account for differences in muscle mass between the pig breeds since activation of this gene may lead to greater myoblast fusion in the commercial breed compared to Piau pigs. Such fusion could explain the different muscularity between breeds in the postnatal periods. |
| first_indexed | 2024-12-14T18:12:43Z |
| format | Article |
| id | doaj.art-c5c6a9c450444b5b821ea025b2cb84e9 |
| institution | Directory Open Access Journal |
| issn | 1678-4685 |
| language | English |
| last_indexed | 2024-12-14T18:12:43Z |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-c5c6a9c450444b5b821ea025b2cb84e92022-12-21T22:52:15ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1678-4685010.1590/1678-4685-GMB-2015-0295S1415-47572016005017102Expression of myogenes in longissimus dorsi muscle during prenatal development in commercial and local Piau pigsEvelyze Pinheiro dos ReisDébora Martins PaixãoOtávio José Bernardes BrustoliniFabyano Fonseca e SilvaWalmir SilvaFlávio Marcos Gomes de AraújoAnna Christina de Matos SalimGuilherme OliveiraSimone Eliza Facioni GuimarãesAbstract This study used qRT-PCR to examine variation in the expression of 13 myogenes during muscle development in four prenatal periods (21, 40, 70 and 90 days post-insemination) in commercial (the three-way Duroc, Landrace and Large-White cross) and local Piau pig breeds that differ in muscle mass. There was no variation in the expression of the CHD8, EID2B, HIF1AN, IKBKB, RSPO3, SOX7 and SUFU genes at the various prenatal ages or between breeds. The MAP2K1 and RBM24 genes showed similar expression between commercial and Piau pigs but greater expression (p < 0.05) in at least one prenatal period. Pair-wise comparisons of prenatal periods in each breed showed that only the CSRP3, LEF1, MRAS and MYOG genes had higher expression (p < 0.05) in at least one prenatal period in commercial and Piau pigs. Overall, these results identified the LEF1 gene as a primary candidate to account for differences in muscle mass between the pig breeds since activation of this gene may lead to greater myoblast fusion in the commercial breed compared to Piau pigs. Such fusion could explain the different muscularity between breeds in the postnatal periods.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016005017102&lng=en&tlng=encommercial linegene expressionmyogenesispigPiau breed |
| spellingShingle | Evelyze Pinheiro dos Reis Débora Martins Paixão Otávio José Bernardes Brustolini Fabyano Fonseca e Silva Walmir Silva Flávio Marcos Gomes de Araújo Anna Christina de Matos Salim Guilherme Oliveira Simone Eliza Facioni Guimarães Expression of myogenes in longissimus dorsi muscle during prenatal development in commercial and local Piau pigs Genetics and Molecular Biology commercial line gene expression myogenesis pig Piau breed |
| title | Expression of myogenes in longissimus dorsi muscle during prenatal development in commercial and local Piau pigs |
| title_full | Expression of myogenes in longissimus dorsi muscle during prenatal development in commercial and local Piau pigs |
| title_fullStr | Expression of myogenes in longissimus dorsi muscle during prenatal development in commercial and local Piau pigs |
| title_full_unstemmed | Expression of myogenes in longissimus dorsi muscle during prenatal development in commercial and local Piau pigs |
| title_short | Expression of myogenes in longissimus dorsi muscle during prenatal development in commercial and local Piau pigs |
| title_sort | expression of myogenes in longissimus dorsi muscle during prenatal development in commercial and local piau pigs |
| topic | commercial line gene expression myogenesis pig Piau breed |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016005017102&lng=en&tlng=en |
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