Minor histocompatibility antigens HA‐8 and PANE1 in the TUNISIAN population
Abstract Background Minor histocompatibility antigens (mHAgs) are endogenous immunogenic peptides initially identified due to complications detected in several contexts of HLA geno‐identical hematopoietic stem cell transplantation (HSCT). In this study, we chose to examine the molecular polymorphism...
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| Format: | Article |
| Language: | English |
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Wiley
2022-11-01
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| Series: | Molecular Genetics & Genomic Medicine |
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| Online Access: | https://doi.org/10.1002/mgg3.2050 |
| _version_ | 1797984294218498048 |
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| author | Rahma Said Mohamed Hichem Sellami Houda Kaabi Slama Hmida |
| author_facet | Rahma Said Mohamed Hichem Sellami Houda Kaabi Slama Hmida |
| author_sort | Rahma Said |
| collection | DOAJ |
| description | Abstract Background Minor histocompatibility antigens (mHAgs) are endogenous immunogenic peptides initially identified due to complications detected in several contexts of HLA geno‐identical hematopoietic stem cell transplantation (HSCT). In this study, we chose to examine the molecular polymorphism of the mHAgs HA‐8 and PANE1 in the Tunisian population. Material and Methods This study was conducted on 150 healthy and unrelated individuals. The DNA extraction and Sequence‐Specific Primers PCR (PCR‐SSP) methods were used for the molecular genotyping of the selected SNPs: PUM3 (rs2173904) and CENPM (rs5758511). Results Our results show that, 94% of Tunisians are carriers of the PANE1R allele (immunogenic variant of the PANE1 mHAg) and 68% of Tunisians are carriers of the HA‐8R allele (immunogenic variant of the HA‐8 mHAg). Furthermore, this study shows that about 5% of the Tunisians are carrier of the PANE1R antigen and its HLA molecule of presentation (the PANE1R/HLA‐A*0301 combination). However, only 2% of Tunisians are carrier of the HA‐8R/HLA‐A*0201 combination, that is, the HA8 immunogenic variant and its specific HLA molecule of presentation. Conclusion Our results are close to those reported in Caucasian, Asiatic, and African populations, this may be explained by the historical events experienced by Tunisia for millennia. These results could be used for further clinical and anthropological studies. |
| first_indexed | 2024-04-11T06:59:19Z |
| format | Article |
| id | doaj.art-c5cac86e0f4a4de48b46b9773b45299d |
| institution | Directory Open Access Journal |
| issn | 2324-9269 |
| language | English |
| last_indexed | 2024-04-11T06:59:19Z |
| publishDate | 2022-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Molecular Genetics & Genomic Medicine |
| spelling | doaj.art-c5cac86e0f4a4de48b46b9773b45299d2022-12-22T04:38:52ZengWileyMolecular Genetics & Genomic Medicine2324-92692022-11-011011n/an/a10.1002/mgg3.2050Minor histocompatibility antigens HA‐8 and PANE1 in the TUNISIAN populationRahma Said0Mohamed Hichem Sellami1Houda Kaabi2Slama Hmida3Department of Immunohaematology National Blood Transfusion Center of Tunis Tunis TunisiaDepartment of Immunohaematology National Blood Transfusion Center of Tunis Tunis TunisiaDepartment of Immunohaematology National Blood Transfusion Center of Tunis Tunis TunisiaDepartment of Immunohaematology National Blood Transfusion Center of Tunis Tunis TunisiaAbstract Background Minor histocompatibility antigens (mHAgs) are endogenous immunogenic peptides initially identified due to complications detected in several contexts of HLA geno‐identical hematopoietic stem cell transplantation (HSCT). In this study, we chose to examine the molecular polymorphism of the mHAgs HA‐8 and PANE1 in the Tunisian population. Material and Methods This study was conducted on 150 healthy and unrelated individuals. The DNA extraction and Sequence‐Specific Primers PCR (PCR‐SSP) methods were used for the molecular genotyping of the selected SNPs: PUM3 (rs2173904) and CENPM (rs5758511). Results Our results show that, 94% of Tunisians are carriers of the PANE1R allele (immunogenic variant of the PANE1 mHAg) and 68% of Tunisians are carriers of the HA‐8R allele (immunogenic variant of the HA‐8 mHAg). Furthermore, this study shows that about 5% of the Tunisians are carrier of the PANE1R antigen and its HLA molecule of presentation (the PANE1R/HLA‐A*0301 combination). However, only 2% of Tunisians are carrier of the HA‐8R/HLA‐A*0201 combination, that is, the HA8 immunogenic variant and its specific HLA molecule of presentation. Conclusion Our results are close to those reported in Caucasian, Asiatic, and African populations, this may be explained by the historical events experienced by Tunisia for millennia. These results could be used for further clinical and anthropological studies.https://doi.org/10.1002/mgg3.2050HA‐8hematopoietic stem cell transplantationminor histocompatibility antigensPANE1SNP |
| spellingShingle | Rahma Said Mohamed Hichem Sellami Houda Kaabi Slama Hmida Minor histocompatibility antigens HA‐8 and PANE1 in the TUNISIAN population Molecular Genetics & Genomic Medicine HA‐8 hematopoietic stem cell transplantation minor histocompatibility antigens PANE1 SNP |
| title | Minor histocompatibility antigens HA‐8 and PANE1 in the TUNISIAN population |
| title_full | Minor histocompatibility antigens HA‐8 and PANE1 in the TUNISIAN population |
| title_fullStr | Minor histocompatibility antigens HA‐8 and PANE1 in the TUNISIAN population |
| title_full_unstemmed | Minor histocompatibility antigens HA‐8 and PANE1 in the TUNISIAN population |
| title_short | Minor histocompatibility antigens HA‐8 and PANE1 in the TUNISIAN population |
| title_sort | minor histocompatibility antigens ha 8 and pane1 in the tunisian population |
| topic | HA‐8 hematopoietic stem cell transplantation minor histocompatibility antigens PANE1 SNP |
| url | https://doi.org/10.1002/mgg3.2050 |
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