Identification of Vimentin as a Potential Therapeutic Target against HIV Infection
A combination of antiviral drugs known as antiretroviral therapy (ART) has shown effectiveness against the human immunodeficiency virus (HIV). ART has markedly decreased mortality and morbidity among HIV-infected patients, having even reduced HIV transmission. However, an important current disadvant...
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| Format: | Article |
| Language: | English |
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MDPI AG
2016-06-01
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| Series: | Viruses |
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| Online Access: | http://www.mdpi.com/1999-4915/8/6/98 |
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| author | Celia Fernández-Ortega Anna Ramírez Dionne Casillas Taimi Paneque Raimundo Ubieta Marta Dubed Leonor Navea Lila Castellanos-Serra Carlos Duarte Viviana Falcon Osvaldo Reyes Hilda Garay Eladio Silva Enrique Noa Yassel Ramos Vladimir Besada Lázaro Betancourt |
| author_facet | Celia Fernández-Ortega Anna Ramírez Dionne Casillas Taimi Paneque Raimundo Ubieta Marta Dubed Leonor Navea Lila Castellanos-Serra Carlos Duarte Viviana Falcon Osvaldo Reyes Hilda Garay Eladio Silva Enrique Noa Yassel Ramos Vladimir Besada Lázaro Betancourt |
| author_sort | Celia Fernández-Ortega |
| collection | DOAJ |
| description | A combination of antiviral drugs known as antiretroviral therapy (ART) has shown effectiveness against the human immunodeficiency virus (HIV). ART has markedly decreased mortality and morbidity among HIV-infected patients, having even reduced HIV transmission. However, an important current disadvantage, resistance development, remains to be solved. Hope is focused on developing drugs against cellular targets. This strategy is expected to prevent the emergence of viral resistance. In this study, using a comparative proteomic approach in MT4 cells treated with an anti-HIV leukocyte extract, we identified vimentin, a molecule forming intermediate filaments in the cell, as a possible target against HIV infection. We demonstrated a strong reduction of an HIV-1 based lentivirus expressing the enhanced green fluorescent protein (eGFP) in vimentin knockdown cells, and a noteworthy decrease of HIV-1 capsid protein antigen (CAp24) in those cells using a multiround infectivity assay. Electron micrographs showed changes in the structure of intermediate filaments when MT4 cells were treated with an anti-HIV leukocyte extract. Changes in the structure of intermediate filaments were also observed in vimentin knockdown MT4 cells. A synthetic peptide derived from a cytoskeleton protein showed potent inhibitory activity on HIV-1 infection, and low cytotoxicity. Our data suggest that vimentin can be a suitable target to inhibit HIV-1. |
| first_indexed | 2024-04-13T20:23:17Z |
| format | Article |
| id | doaj.art-c5cc3b38b55a492399329cb042f50564 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-04-13T20:23:17Z |
| publishDate | 2016-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-c5cc3b38b55a492399329cb042f505642022-12-22T02:31:29ZengMDPI AGViruses1999-49152016-06-01869810.3390/v8060098v8060098Identification of Vimentin as a Potential Therapeutic Target against HIV InfectionCelia Fernández-Ortega0Anna Ramírez1Dionne Casillas2Taimi Paneque3Raimundo Ubieta4Marta Dubed5Leonor Navea6Lila Castellanos-Serra7Carlos Duarte8Viviana Falcon9Osvaldo Reyes10Hilda Garay11Eladio Silva12Enrique Noa13Yassel Ramos14Vladimir Besada15Lázaro Betancourt16Center for Genetic Engineering and Biotechnology, Ave 31/158 and 190, Cubanacán, Playa, P.O. Box 6162, Havana 10600, CubaCenter for Genetic Engineering and Biotechnology, Ave 31/158 and 190, Cubanacán, Playa, P.O. Box 6162, Havana 10600, CubaCenter for Genetic Engineering and Biotechnology, Ave 31/158 and 190, Cubanacán, Playa, P.O. Box 6162, Havana 10600, CubaCenter for Genetic Engineering and Biotechnology, Ave 31/158 and 190, Cubanacán, Playa, P.O. Box 6162, Havana 10600, CubaCenter for Genetic Engineering and Biotechnology, Ave 31/158 and 190, Cubanacán, Playa, P.O. Box 6162, Havana 10600, CubaLaboratory for AIDS Research, Carretera de Tapaste y Autopista Nacional, San José de las Lajas, Mayabeque CP 32700, CubaLaboratory for AIDS Research, Carretera de Tapaste y Autopista Nacional, San José de las Lajas, Mayabeque CP 32700, CubaCenter for Genetic Engineering and Biotechnology, Ave 31/158 and 190, Cubanacán, Playa, P.O. Box 6162, Havana 10600, CubaCenter for Genetic Engineering and Biotechnology, Ave 31/158 and 190, Cubanacán, Playa, P.O. Box 6162, Havana 10600, CubaCenter for Genetic Engineering and Biotechnology, Ave 31/158 and 190, Cubanacán, Playa, P.O. Box 6162, Havana 10600, CubaCenter for Genetic Engineering and Biotechnology, Ave 31/158 and 190, Cubanacán, Playa, P.O. Box 6162, Havana 10600, CubaCenter for Genetic Engineering and Biotechnology, Ave 31/158 and 190, Cubanacán, Playa, P.O. Box 6162, Havana 10600, CubaLaboratory for AIDS Research, Carretera de Tapaste y Autopista Nacional, San José de las Lajas, Mayabeque CP 32700, CubaLaboratory for AIDS Research, Carretera de Tapaste y Autopista Nacional, San José de las Lajas, Mayabeque CP 32700, CubaCenter for Genetic Engineering and Biotechnology, Ave 31/158 and 190, Cubanacán, Playa, P.O. Box 6162, Havana 10600, CubaCenter for Genetic Engineering and Biotechnology, Ave 31/158 and 190, Cubanacán, Playa, P.O. Box 6162, Havana 10600, CubaCenter for Genetic Engineering and Biotechnology, Ave 31/158 and 190, Cubanacán, Playa, P.O. Box 6162, Havana 10600, CubaA combination of antiviral drugs known as antiretroviral therapy (ART) has shown effectiveness against the human immunodeficiency virus (HIV). ART has markedly decreased mortality and morbidity among HIV-infected patients, having even reduced HIV transmission. However, an important current disadvantage, resistance development, remains to be solved. Hope is focused on developing drugs against cellular targets. This strategy is expected to prevent the emergence of viral resistance. In this study, using a comparative proteomic approach in MT4 cells treated with an anti-HIV leukocyte extract, we identified vimentin, a molecule forming intermediate filaments in the cell, as a possible target against HIV infection. We demonstrated a strong reduction of an HIV-1 based lentivirus expressing the enhanced green fluorescent protein (eGFP) in vimentin knockdown cells, and a noteworthy decrease of HIV-1 capsid protein antigen (CAp24) in those cells using a multiround infectivity assay. Electron micrographs showed changes in the structure of intermediate filaments when MT4 cells were treated with an anti-HIV leukocyte extract. Changes in the structure of intermediate filaments were also observed in vimentin knockdown MT4 cells. A synthetic peptide derived from a cytoskeleton protein showed potent inhibitory activity on HIV-1 infection, and low cytotoxicity. Our data suggest that vimentin can be a suitable target to inhibit HIV-1.http://www.mdpi.com/1999-4915/8/6/98leukocyte extractvimentinintermediate filamentscytoskeletonHIVanti-HIV activityproteomics |
| spellingShingle | Celia Fernández-Ortega Anna Ramírez Dionne Casillas Taimi Paneque Raimundo Ubieta Marta Dubed Leonor Navea Lila Castellanos-Serra Carlos Duarte Viviana Falcon Osvaldo Reyes Hilda Garay Eladio Silva Enrique Noa Yassel Ramos Vladimir Besada Lázaro Betancourt Identification of Vimentin as a Potential Therapeutic Target against HIV Infection Viruses leukocyte extract vimentin intermediate filaments cytoskeleton HIV anti-HIV activity proteomics |
| title | Identification of Vimentin as a Potential Therapeutic Target against HIV Infection |
| title_full | Identification of Vimentin as a Potential Therapeutic Target against HIV Infection |
| title_fullStr | Identification of Vimentin as a Potential Therapeutic Target against HIV Infection |
| title_full_unstemmed | Identification of Vimentin as a Potential Therapeutic Target against HIV Infection |
| title_short | Identification of Vimentin as a Potential Therapeutic Target against HIV Infection |
| title_sort | identification of vimentin as a potential therapeutic target against hiv infection |
| topic | leukocyte extract vimentin intermediate filaments cytoskeleton HIV anti-HIV activity proteomics |
| url | http://www.mdpi.com/1999-4915/8/6/98 |
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