Natural Agents against Bovine Mastitis Pathogens
Bovine mastitis is the most widespread and economically important disease worldwide. The present study aimed to determine bioactive compounds in two essential oils (EOs) from wild <i>(Thymus serpyllum)</i> and common thyme <i>(Thymus vulgaris)</i> and to assess the antioxidan...
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| Jezik: | English |
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MDPI AG
2021-02-01
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| Serija: | Antibiotics |
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| Online pristup: | https://www.mdpi.com/2079-6382/10/2/205 |
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| author | Zorana Kovačević Miodrag Radinović Ivana Čabarkapa Nebojša Kladar Biljana Božin |
| author_facet | Zorana Kovačević Miodrag Radinović Ivana Čabarkapa Nebojša Kladar Biljana Božin |
| author_sort | Zorana Kovačević |
| collection | DOAJ |
| description | Bovine mastitis is the most widespread and economically important disease worldwide. The present study aimed to determine bioactive compounds in two essential oils (EOs) from wild <i>(Thymus serpyllum)</i> and common thyme <i>(Thymus vulgaris)</i> and to assess the antioxidant potential as well as antibacterial efficacy of the EOs against mastitis-associated bacteria. The study also included antibiotic susceptibility tests. The strains were previously isolated from lactating animals with clinical and subclinical mastitis. The antioxidant potential of the commercial EOs of wild and common thyme was evaluated by five in vitro assays. The antibacterial activity was performed using the microdilution technique, while antibiotic susceptibility testing was performed by the Kirby–Bauer disc diffusion method. The dominant compound in wild thyme was thymol (45.22%), followed by <i>p</i>-cymene (23.83%) and γ-terpinene (3.12%), while in common thyme, it was thymol (54.17%), followed by γ-terpinene (22.18%) and <i>p</i>-cymene (16.66%). Among the fourteen mastitis-associated bacteria, strain IX <i>Streptococcus</i> spp. (β-hemolytic) was the most sensitive to the tested EOs (minimum inhibitory concentration (MIC)/minimal bactericidal concentration (MBC) were 0.78/1.56 and 0.39/0.78 mg/mL for <i>T. serpyllum</i> (TS) and <i>T. vulgaris (TV)</i>, respectively). Regarding <i>Streptococcus</i> spp. β heamoliticus, MICs for <i>TS</i> ranged from 0.78 to 1.56 mg/mL, while for the same oil, MBCs ranged from 1.56 to 12.5 mg/mL. In the case of <i>T. vulgaris,</i> MICs ranged from 0.39 to 3.125 mg/ mL, while MBCs ranged from 3.125 to 6.25 mg/mL. TV is more active against <i>E. coli, E. sakazakii,</i> and <i>Streptococcus</i> spp., while it is less effective against <i>Staphylococcus</i> spp. than TS. The study revealed that the tested EOs possess remarkable antioxidative and antibacterial activities and could be used in the development of pharmaceutical formulation as an alternative to conventional mastitis therapy. |
| first_indexed | 2024-03-09T00:45:03Z |
| format | Article |
| id | doaj.art-c5cdfa11edd74990b2b5d41a7ce6b3df |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T00:45:03Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-c5cdfa11edd74990b2b5d41a7ce6b3df2023-12-11T17:36:01ZengMDPI AGAntibiotics2079-63822021-02-0110220510.3390/antibiotics10020205Natural Agents against Bovine Mastitis PathogensZorana Kovačević0Miodrag Radinović1Ivana Čabarkapa2Nebojša Kladar3Biljana Božin4Department of Veterinary Medicine, Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovica 8, 21000 Novi Sad, SerbiaDepartment of Veterinary Medicine, Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovica 8, 21000 Novi Sad, SerbiaInstitute of Food Technology, University of Novi Sad, Bulevar cara Lazara 1, 21000 Novi Sad, SerbiaCenter for Medical and Pharmaceutical Investigations and Quality Control/Department of Pharmacy, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21000 Novi Sad, SerbiaCenter for Medical and Pharmaceutical Investigations and Quality Control/Department of Pharmacy, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21000 Novi Sad, SerbiaBovine mastitis is the most widespread and economically important disease worldwide. The present study aimed to determine bioactive compounds in two essential oils (EOs) from wild <i>(Thymus serpyllum)</i> and common thyme <i>(Thymus vulgaris)</i> and to assess the antioxidant potential as well as antibacterial efficacy of the EOs against mastitis-associated bacteria. The study also included antibiotic susceptibility tests. The strains were previously isolated from lactating animals with clinical and subclinical mastitis. The antioxidant potential of the commercial EOs of wild and common thyme was evaluated by five in vitro assays. The antibacterial activity was performed using the microdilution technique, while antibiotic susceptibility testing was performed by the Kirby–Bauer disc diffusion method. The dominant compound in wild thyme was thymol (45.22%), followed by <i>p</i>-cymene (23.83%) and γ-terpinene (3.12%), while in common thyme, it was thymol (54.17%), followed by γ-terpinene (22.18%) and <i>p</i>-cymene (16.66%). Among the fourteen mastitis-associated bacteria, strain IX <i>Streptococcus</i> spp. (β-hemolytic) was the most sensitive to the tested EOs (minimum inhibitory concentration (MIC)/minimal bactericidal concentration (MBC) were 0.78/1.56 and 0.39/0.78 mg/mL for <i>T. serpyllum</i> (TS) and <i>T. vulgaris (TV)</i>, respectively). Regarding <i>Streptococcus</i> spp. β heamoliticus, MICs for <i>TS</i> ranged from 0.78 to 1.56 mg/mL, while for the same oil, MBCs ranged from 1.56 to 12.5 mg/mL. In the case of <i>T. vulgaris,</i> MICs ranged from 0.39 to 3.125 mg/ mL, while MBCs ranged from 3.125 to 6.25 mg/mL. TV is more active against <i>E. coli, E. sakazakii,</i> and <i>Streptococcus</i> spp., while it is less effective against <i>Staphylococcus</i> spp. than TS. The study revealed that the tested EOs possess remarkable antioxidative and antibacterial activities and could be used in the development of pharmaceutical formulation as an alternative to conventional mastitis therapy.https://www.mdpi.com/2079-6382/10/2/205antibacterial activityessential oilmastitis causing bacteriaantioxidantthymolantibiotics |
| spellingShingle | Zorana Kovačević Miodrag Radinović Ivana Čabarkapa Nebojša Kladar Biljana Božin Natural Agents against Bovine Mastitis Pathogens Antibiotics antibacterial activity essential oil mastitis causing bacteria antioxidant thymol antibiotics |
| title | Natural Agents against Bovine Mastitis Pathogens |
| title_full | Natural Agents against Bovine Mastitis Pathogens |
| title_fullStr | Natural Agents against Bovine Mastitis Pathogens |
| title_full_unstemmed | Natural Agents against Bovine Mastitis Pathogens |
| title_short | Natural Agents against Bovine Mastitis Pathogens |
| title_sort | natural agents against bovine mastitis pathogens |
| topic | antibacterial activity essential oil mastitis causing bacteria antioxidant thymol antibiotics |
| url | https://www.mdpi.com/2079-6382/10/2/205 |
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