The effects of presynch-10 and ovsynch on some endometrial tolland nod-like receptor gene expressions in repeat breeder cows
Subclinical endometritis (SE) is one cause of repeat breeder syndrome (RBS). When endometrial cells come into contact with antigens during inflammatory events like endometritis, they act like innate immune system cells. Ovsynch-based therapies stimulate the innate immune system, which is how they ma...
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2019-08-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=2637 |
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| author | Ali Reha AĞAOĞLU Özgecan KORKMAZ AĞAOĞLU Selim ASLAN Mesih KOCAMÜFTÜOĞLU Afşin KÖKER Yunus ÇETİN Örsan GÜNGÖR Dilek ÖZTÜRK Mustafa SAATCI |
| author_facet | Ali Reha AĞAOĞLU Özgecan KORKMAZ AĞAOĞLU Selim ASLAN Mesih KOCAMÜFTÜOĞLU Afşin KÖKER Yunus ÇETİN Örsan GÜNGÖR Dilek ÖZTÜRK Mustafa SAATCI |
| author_sort | Ali Reha AĞAOĞLU |
| collection | DOAJ |
| description | Subclinical endometritis (SE) is one cause of repeat breeder syndrome (RBS). When endometrial cells come into contact with antigens during
inflammatory events like endometritis, they act like innate immune system cells. Ovsynch-based therapies stimulate the innate immune system,
which is how they may be able to cure SE and then RBS. This study aimed to assess the effects of presynch-10 and ovsynch applications on mRNA
expressions of some endometrial Toll- and Nod-like receptors (TLRs and NLRs) in cows with RBS. A total of 40 repeat breeder cows were used in the
study. The presynch-10 and ovsynch protocols were used in group 1 (G1, n=20), and no protocol was used in group 2 (G2, n=20). Endometrial samples
were collected before and after the synchronization protocols in G1. In G2, samples were collected, and after waiting time up to the duration of
synchronization protocol in G1, samples were taken again. The expression profiles of the genes being investigated were examined using RT-qPCR.
NLRC4, NALP3 and TLR4 were basically expressed in all of the samples while NOD2 expression was completely undetectable. There was a statistical
difference when NOD1 and TLR2 expressions in the second samples were compared between G1 and G2 (P<0.05). It was concluded that presynch-10
and ovsynch administration in cows may have a positively effect on the innate immune response and so be effective in the treatment of SE. |
| first_indexed | 2024-03-13T04:38:41Z |
| format | Article |
| id | doaj.art-c5d18d18502c4c72b6e26f9fc8ef4389 |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:38:41Z |
| publishDate | 2019-08-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-c5d18d18502c4c72b6e26f9fc8ef43892023-06-19T06:33:12ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512019-08-0126111112010.9775/kvfd.2019.224282637The effects of presynch-10 and ovsynch on some endometrial tolland nod-like receptor gene expressions in repeat breeder cowsAli Reha AĞAOĞLU0Özgecan KORKMAZ AĞAOĞLU1Selim ASLAN2Mesih KOCAMÜFTÜOĞLU3Afşin KÖKER4Yunus ÇETİN5Örsan GÜNGÖR6Dilek ÖZTÜRK7Mustafa SAATCI8Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology, TR-15030 Burdur - TURKEYMehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Animal Science, TR-15030 Burdur - TURKEYNear East University, Veterinary Faculty, Department of Obstetrics and Gynecology, Nicosia; North Cyprus, Mersin 10, TURKEYMehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology, TR-15030 Burdur - TURKEYMehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology, TR-15030 Burdur - TURKEYMehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology, TR-15030 Burdur - TURKEYMehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology, TR-15030 Burdur - TURKEYMehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Microbiology, TR-15030 Burdur - TURKEYMuğla Sıtkı Koçman University, Fethiye Agriculture Faculty, Department of Animal Science, TR-48000 Muğla - TURKEYSubclinical endometritis (SE) is one cause of repeat breeder syndrome (RBS). When endometrial cells come into contact with antigens during inflammatory events like endometritis, they act like innate immune system cells. Ovsynch-based therapies stimulate the innate immune system, which is how they may be able to cure SE and then RBS. This study aimed to assess the effects of presynch-10 and ovsynch applications on mRNA expressions of some endometrial Toll- and Nod-like receptors (TLRs and NLRs) in cows with RBS. A total of 40 repeat breeder cows were used in the study. The presynch-10 and ovsynch protocols were used in group 1 (G1, n=20), and no protocol was used in group 2 (G2, n=20). Endometrial samples were collected before and after the synchronization protocols in G1. In G2, samples were collected, and after waiting time up to the duration of synchronization protocol in G1, samples were taken again. The expression profiles of the genes being investigated were examined using RT-qPCR. NLRC4, NALP3 and TLR4 were basically expressed in all of the samples while NOD2 expression was completely undetectable. There was a statistical difference when NOD1 and TLR2 expressions in the second samples were compared between G1 and G2 (P<0.05). It was concluded that presynch-10 and ovsynch administration in cows may have a positively effect on the innate immune response and so be effective in the treatment of SE.https://vetdergikafkas.org/pdf.php?id=2637endometritiscowinnate immunitypattern recognition receptors |
| spellingShingle | Ali Reha AĞAOĞLU Özgecan KORKMAZ AĞAOĞLU Selim ASLAN Mesih KOCAMÜFTÜOĞLU Afşin KÖKER Yunus ÇETİN Örsan GÜNGÖR Dilek ÖZTÜRK Mustafa SAATCI The effects of presynch-10 and ovsynch on some endometrial tolland nod-like receptor gene expressions in repeat breeder cows Kafkas Universitesi Veteriner Fakültesi Dergisi endometritis cow innate immunity pattern recognition receptors |
| title | The effects of presynch-10 and ovsynch on some endometrial tolland nod-like receptor gene expressions in repeat breeder cows |
| title_full | The effects of presynch-10 and ovsynch on some endometrial tolland nod-like receptor gene expressions in repeat breeder cows |
| title_fullStr | The effects of presynch-10 and ovsynch on some endometrial tolland nod-like receptor gene expressions in repeat breeder cows |
| title_full_unstemmed | The effects of presynch-10 and ovsynch on some endometrial tolland nod-like receptor gene expressions in repeat breeder cows |
| title_short | The effects of presynch-10 and ovsynch on some endometrial tolland nod-like receptor gene expressions in repeat breeder cows |
| title_sort | effects of presynch 10 and ovsynch on some endometrial tolland nod like receptor gene expressions in repeat breeder cows |
| topic | endometritis cow innate immunity pattern recognition receptors |
| url | https://vetdergikafkas.org/pdf.php?id=2637 |
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