Identification of plasma microRNA expression changes in multiple system atrophy and Parkinson’s disease
Abstract MicroRNAs (miRNAs) are endogenous small (18–25 nt), single-stranded, non-coding RNAs that play key roles in post-transcriptional gene expression regulation. The expression profiles of miRNAs in biofluids and tissues change in various diseases. Multiple system atrophy (MSA) and Parkinson’s d...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-05-01
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| Series: | Molecular Brain |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13041-019-0471-2 |
| _version_ | 1819103880204517376 |
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| author | Hisashi Uwatoko Yuka Hama Ikuko Takahashi Iwata Shinichi Shirai Masaaki Matsushima Ichiro Yabe Jun Utsumi Hidenao Sasaki |
| author_facet | Hisashi Uwatoko Yuka Hama Ikuko Takahashi Iwata Shinichi Shirai Masaaki Matsushima Ichiro Yabe Jun Utsumi Hidenao Sasaki |
| author_sort | Hisashi Uwatoko |
| collection | DOAJ |
| description | Abstract MicroRNAs (miRNAs) are endogenous small (18–25 nt), single-stranded, non-coding RNAs that play key roles in post-transcriptional gene expression regulation. The expression profiles of miRNAs in biofluids and tissues change in various diseases. Multiple system atrophy (MSA) and Parkinson’s disease (PD) are both categorized as α-synucleinopathies and often present with similar clinical manifestations. This study aimed to identify miRNAs that are differently expressed in plasma samples of PD patients, MSA patients, and healthy controls. We used microarray analysis to screen for miRNAs that are up- and down-regulated in these patients and analyzed the relative-quantitative expression levels of the identified miRNAs by reverse transcription quantitative polymerase chain reaction (RT-qPCR). Hsa-miR-671-5p, hsa-miR-19b-3p, and hsa-miR-24-3p showed significantly different expression levels among patients with MSA-C, MSA-P, or PD, and healthy controls. Hsa-miR-671-5p levels were lower in the MSA-P and PD than the MSA-C and control groups, hsa-miR-19b-3p levels were higher in the PD than the other groups, and hsa-miR-24-3p levels were higher in the PD than the MSA-C group. Hsa-miR-671-5p was the first miRNA shown to be expressed differently between MSA-C and MSA-P in plasma. Interestingly, the expression levels of hsa-miR-19b-3p and hsa-miR-24-3p were positively correlated, indicating that these miRNAs may be involved in the same processes in PD pathogenesis. Our findings suggest that hsa-miR-671-5p, hsa-miR-19b-3p, and hsa-miR-24-3p may reflect the pathophysiology or symptoms of PD and MSA. |
| first_indexed | 2024-12-22T01:57:29Z |
| format | Article |
| id | doaj.art-c5d4f82c2c2e4de695ec27524b6f93f0 |
| institution | Directory Open Access Journal |
| issn | 1756-6606 |
| language | English |
| last_indexed | 2024-12-22T01:57:29Z |
| publishDate | 2019-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Brain |
| spelling | doaj.art-c5d4f82c2c2e4de695ec27524b6f93f02022-12-21T18:42:44ZengBMCMolecular Brain1756-66062019-05-0112111010.1186/s13041-019-0471-2Identification of plasma microRNA expression changes in multiple system atrophy and Parkinson’s diseaseHisashi Uwatoko0Yuka Hama1Ikuko Takahashi Iwata2Shinichi Shirai3Masaaki Matsushima4Ichiro Yabe5Jun Utsumi6Hidenao Sasaki7Department of Neurology, Faculty of Medicine and Graduate School of Medicine, Hokkaido UniversityDepartment of Neurology, Faculty of Medicine and Graduate School of Medicine, Hokkaido UniversityDepartment of Neurology, Faculty of Medicine and Graduate School of Medicine, Hokkaido UniversityDepartment of Neurology, Faculty of Medicine and Graduate School of Medicine, Hokkaido UniversityDepartment of Neurology, Faculty of Medicine and Graduate School of Medicine, Hokkaido UniversityDepartment of Neurology, Faculty of Medicine and Graduate School of Medicine, Hokkaido UniversityDepartment of Neurology, Faculty of Medicine and Graduate School of Medicine, Hokkaido UniversityDepartment of Neurology, Faculty of Medicine and Graduate School of Medicine, Hokkaido UniversityAbstract MicroRNAs (miRNAs) are endogenous small (18–25 nt), single-stranded, non-coding RNAs that play key roles in post-transcriptional gene expression regulation. The expression profiles of miRNAs in biofluids and tissues change in various diseases. Multiple system atrophy (MSA) and Parkinson’s disease (PD) are both categorized as α-synucleinopathies and often present with similar clinical manifestations. This study aimed to identify miRNAs that are differently expressed in plasma samples of PD patients, MSA patients, and healthy controls. We used microarray analysis to screen for miRNAs that are up- and down-regulated in these patients and analyzed the relative-quantitative expression levels of the identified miRNAs by reverse transcription quantitative polymerase chain reaction (RT-qPCR). Hsa-miR-671-5p, hsa-miR-19b-3p, and hsa-miR-24-3p showed significantly different expression levels among patients with MSA-C, MSA-P, or PD, and healthy controls. Hsa-miR-671-5p levels were lower in the MSA-P and PD than the MSA-C and control groups, hsa-miR-19b-3p levels were higher in the PD than the other groups, and hsa-miR-24-3p levels were higher in the PD than the MSA-C group. Hsa-miR-671-5p was the first miRNA shown to be expressed differently between MSA-C and MSA-P in plasma. Interestingly, the expression levels of hsa-miR-19b-3p and hsa-miR-24-3p were positively correlated, indicating that these miRNAs may be involved in the same processes in PD pathogenesis. Our findings suggest that hsa-miR-671-5p, hsa-miR-19b-3p, and hsa-miR-24-3p may reflect the pathophysiology or symptoms of PD and MSA.http://link.springer.com/article/10.1186/s13041-019-0471-2Multiple system atrophyParkinson’s diseasemicroRNAPlasmaMicroarrayQuantitative polymerase chain reaction |
| spellingShingle | Hisashi Uwatoko Yuka Hama Ikuko Takahashi Iwata Shinichi Shirai Masaaki Matsushima Ichiro Yabe Jun Utsumi Hidenao Sasaki Identification of plasma microRNA expression changes in multiple system atrophy and Parkinson’s disease Molecular Brain Multiple system atrophy Parkinson’s disease microRNA Plasma Microarray Quantitative polymerase chain reaction |
| title | Identification of plasma microRNA expression changes in multiple system atrophy and Parkinson’s disease |
| title_full | Identification of plasma microRNA expression changes in multiple system atrophy and Parkinson’s disease |
| title_fullStr | Identification of plasma microRNA expression changes in multiple system atrophy and Parkinson’s disease |
| title_full_unstemmed | Identification of plasma microRNA expression changes in multiple system atrophy and Parkinson’s disease |
| title_short | Identification of plasma microRNA expression changes in multiple system atrophy and Parkinson’s disease |
| title_sort | identification of plasma microrna expression changes in multiple system atrophy and parkinson s disease |
| topic | Multiple system atrophy Parkinson’s disease microRNA Plasma Microarray Quantitative polymerase chain reaction |
| url | http://link.springer.com/article/10.1186/s13041-019-0471-2 |
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