Maturation of subtilisin-like protease NbSLP1 from microsporidia Nosema bombycis
Microsporidia are obligate intracellular parasites and possess a unique way of invading hosts, namely germination. Microsporidia are able to infect almost all animal cells by germination. During the process, the polar tube extrudes from the spores within, thus injecting infectious sporoplasm into th...
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.897509/full |
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| author | Rong Wang Qingyan Li Fangyan Liu Xiaoqun Dang Quan Sun Xiaotian Sheng Mingyu Hu Jialing Bao Jie Chen Guoqing Pan Zeyang Zhou Zeyang Zhou |
| author_facet | Rong Wang Qingyan Li Fangyan Liu Xiaoqun Dang Quan Sun Xiaotian Sheng Mingyu Hu Jialing Bao Jie Chen Guoqing Pan Zeyang Zhou Zeyang Zhou |
| author_sort | Rong Wang |
| collection | DOAJ |
| description | Microsporidia are obligate intracellular parasites and possess a unique way of invading hosts, namely germination. Microsporidia are able to infect almost all animal cells by germination. During the process, the polar tube extrudes from the spores within, thus injecting infectious sporoplasm into the host cells. Previous studies indicated that subtilisin-like protease 1 (NbSLP1) of microsporidia Nosema bombycis were located at the polar cap of germinated spores where the polar tube extrusion. We hypothesized that NbSLP1 is an essential player in the germination process. Normally, SLP need to be activated by autoproteolysis under conditions. In this study, we found that the signal peptide of NbSLP1 affected the activation of protease, two self-cleavage sites were involved in NbSLP1 maturation between Ala104Asp105 and Ala124Asp125 respectively. Mutants at catalytic triad of NbSLP1 confirmed the decreasing of autoproteolysis. This study demonstrates that intramolecular proteolysis is required for NbSLP1 maturation. The protease undergoes a series of sequential N-terminal cleavage events to generate the mature enzyme. Like other subtilisin-like enzymes, catalytic triad of NbSLP1 are significant for the self-activation of NbSLP1. In conclusion, clarifying the maturation of NbSLP1 will be valuable for understanding the polar tube ejection mechanism of germination. |
| first_indexed | 2024-04-11T22:01:47Z |
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| language | English |
| last_indexed | 2024-04-11T22:01:47Z |
| publishDate | 2022-08-01 |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-c5d746a3fa114a5e91f8a3ed374ecb242022-12-22T04:00:55ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-08-011210.3389/fcimb.2022.897509897509Maturation of subtilisin-like protease NbSLP1 from microsporidia Nosema bombycisRong Wang0Qingyan Li1Fangyan Liu2Xiaoqun Dang3Quan Sun4Xiaotian Sheng5Mingyu Hu6Jialing Bao7Jie Chen8Guoqing Pan9Zeyang Zhou10Zeyang Zhou11State Key Laboratory of Silkworm Genome Biology, Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing, ChinaState Key Laboratory of Silkworm Genome Biology, Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing, ChinaState Key Laboratory of Silkworm Genome Biology, Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing, ChinaCollege of Life Sciences, Chongqing Normal University, Chongqing, ChinaState Key Laboratory of Silkworm Genome Biology, Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing, ChinaState Key Laboratory of Silkworm Genome Biology, Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing, ChinaState Key Laboratory of Silkworm Genome Biology, Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing, ChinaState Key Laboratory of Silkworm Genome Biology, Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing, ChinaState Key Laboratory of Silkworm Genome Biology, Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing, ChinaState Key Laboratory of Silkworm Genome Biology, Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing, ChinaState Key Laboratory of Silkworm Genome Biology, Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing, ChinaCollege of Life Sciences, Chongqing Normal University, Chongqing, ChinaMicrosporidia are obligate intracellular parasites and possess a unique way of invading hosts, namely germination. Microsporidia are able to infect almost all animal cells by germination. During the process, the polar tube extrudes from the spores within, thus injecting infectious sporoplasm into the host cells. Previous studies indicated that subtilisin-like protease 1 (NbSLP1) of microsporidia Nosema bombycis were located at the polar cap of germinated spores where the polar tube extrusion. We hypothesized that NbSLP1 is an essential player in the germination process. Normally, SLP need to be activated by autoproteolysis under conditions. In this study, we found that the signal peptide of NbSLP1 affected the activation of protease, two self-cleavage sites were involved in NbSLP1 maturation between Ala104Asp105 and Ala124Asp125 respectively. Mutants at catalytic triad of NbSLP1 confirmed the decreasing of autoproteolysis. This study demonstrates that intramolecular proteolysis is required for NbSLP1 maturation. The protease undergoes a series of sequential N-terminal cleavage events to generate the mature enzyme. Like other subtilisin-like enzymes, catalytic triad of NbSLP1 are significant for the self-activation of NbSLP1. In conclusion, clarifying the maturation of NbSLP1 will be valuable for understanding the polar tube ejection mechanism of germination.https://www.frontiersin.org/articles/10.3389/fcimb.2022.897509/fullmicrosporidiaNosema bombicyssubtilisin-like proteaseautoproteolysisgermination |
| spellingShingle | Rong Wang Qingyan Li Fangyan Liu Xiaoqun Dang Quan Sun Xiaotian Sheng Mingyu Hu Jialing Bao Jie Chen Guoqing Pan Zeyang Zhou Zeyang Zhou Maturation of subtilisin-like protease NbSLP1 from microsporidia Nosema bombycis Frontiers in Cellular and Infection Microbiology microsporidia Nosema bombicys subtilisin-like protease autoproteolysis germination |
| title | Maturation of subtilisin-like protease NbSLP1 from microsporidia Nosema bombycis |
| title_full | Maturation of subtilisin-like protease NbSLP1 from microsporidia Nosema bombycis |
| title_fullStr | Maturation of subtilisin-like protease NbSLP1 from microsporidia Nosema bombycis |
| title_full_unstemmed | Maturation of subtilisin-like protease NbSLP1 from microsporidia Nosema bombycis |
| title_short | Maturation of subtilisin-like protease NbSLP1 from microsporidia Nosema bombycis |
| title_sort | maturation of subtilisin like protease nbslp1 from microsporidia nosema bombycis |
| topic | microsporidia Nosema bombicys subtilisin-like protease autoproteolysis germination |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.897509/full |
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