Virulence Genes and Antibiotic Resistance Patterns of Bacillus cereus Isolated from Egyptian Milk, Milk Powder, and Ice Cream
One of the most common food poisoning illnesses globally is caused by Bacillus cereus, which causes emetic and diarrheal food poisoning. Forty–two (42%) out of 100 samples from raw milk, powdered milk, ice cream, and pasteurized milk were positive for B. cereus, with a high prevalence in raw milk a...
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| Format: | Article |
| Language: | English |
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Assiut University
2023-06-01
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| Series: | Journal of Advanced Veterinary Research |
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| Online Access: | https://advetresearch.com/index.php/AVR/article/view/1276 |
| _version_ | 1797657073338548224 |
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| author | Heba A. Dowidar Amira H. El-Baz |
| author_facet | Heba A. Dowidar Amira H. El-Baz |
| author_sort | Heba A. Dowidar |
| collection | DOAJ |
| description |
One of the most common food poisoning illnesses globally is caused by Bacillus cereus, which causes emetic and diarrheal food poisoning. Forty–two (42%) out of 100 samples from raw milk, powdered milk, ice cream, and pasteurized milk were positive for B. cereus, with a high prevalence in raw milk at 64%. Three virulence genes (nheA, cytK, and hblC genes) were characterized among 42 B. cereus isolates with variable frequencies. Detection of the nheA gene showed a high level of 90.4%, followed by the cytK gene in percentage at 50%, and the hblC gene at 47.6%. All examined strains were resistant to Penicillin, Oxacillin, then Cefixime and Ampicillin (85.7%), followed by Nalidixic acid (73.8%), Sulphamethoxazol-Trimethoprim(61.9%), and Oxytetracycline and Cephalotin (52.3%) whilst sensitive to Gentamicin (75%), followed by Enrofloxacin and Erythromycin (50%). Unfortunately, all examined strains are MDR and show 23 resistance patterns, this represents a real health malice for the people of Egypt. The obtained results demonstrated the rise of pathogenic B. cereus, a virulent organism that is multidrug-resistant, in Egypt's retail milk and milk products.
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| first_indexed | 2024-03-11T17:39:07Z |
| format | Article |
| id | doaj.art-c5d829e364b04fc29d9524029e380e3e |
| institution | Directory Open Access Journal |
| issn | 2090-6269 2090-6277 |
| language | English |
| last_indexed | 2024-03-11T17:39:07Z |
| publishDate | 2023-06-01 |
| publisher | Assiut University |
| record_format | Article |
| series | Journal of Advanced Veterinary Research |
| spelling | doaj.art-c5d829e364b04fc29d9524029e380e3e2023-10-18T14:14:15ZengAssiut UniversityJournal of Advanced Veterinary Research2090-62692090-62772023-06-01134Virulence Genes and Antibiotic Resistance Patterns of Bacillus cereus Isolated from Egyptian Milk, Milk Powder, and Ice CreamHeba A. Dowidar0Amira H. El-Baz1Department of Medical Laboratory, Higher Institute of Technology for Applied Health Science, Badr Institute for Science and Technology, Cairo, Egypt.Department of Food Hygiene, Safety, and Technology, Faculty of Veterinary Medicine, Mansoura University, Egypt. One of the most common food poisoning illnesses globally is caused by Bacillus cereus, which causes emetic and diarrheal food poisoning. Forty–two (42%) out of 100 samples from raw milk, powdered milk, ice cream, and pasteurized milk were positive for B. cereus, with a high prevalence in raw milk at 64%. Three virulence genes (nheA, cytK, and hblC genes) were characterized among 42 B. cereus isolates with variable frequencies. Detection of the nheA gene showed a high level of 90.4%, followed by the cytK gene in percentage at 50%, and the hblC gene at 47.6%. All examined strains were resistant to Penicillin, Oxacillin, then Cefixime and Ampicillin (85.7%), followed by Nalidixic acid (73.8%), Sulphamethoxazol-Trimethoprim(61.9%), and Oxytetracycline and Cephalotin (52.3%) whilst sensitive to Gentamicin (75%), followed by Enrofloxacin and Erythromycin (50%). Unfortunately, all examined strains are MDR and show 23 resistance patterns, this represents a real health malice for the people of Egypt. The obtained results demonstrated the rise of pathogenic B. cereus, a virulent organism that is multidrug-resistant, in Egypt's retail milk and milk products. https://advetresearch.com/index.php/AVR/article/view/1276 Bacillus cereus Virulence genes Antibiotic resistanceDairy products |
| spellingShingle | Heba A. Dowidar Amira H. El-Baz Virulence Genes and Antibiotic Resistance Patterns of Bacillus cereus Isolated from Egyptian Milk, Milk Powder, and Ice Cream Journal of Advanced Veterinary Research Bacillus cereus Virulence genes Antibiotic resistance Dairy products |
| title | Virulence Genes and Antibiotic Resistance Patterns of Bacillus cereus Isolated from Egyptian Milk, Milk Powder, and Ice Cream |
| title_full | Virulence Genes and Antibiotic Resistance Patterns of Bacillus cereus Isolated from Egyptian Milk, Milk Powder, and Ice Cream |
| title_fullStr | Virulence Genes and Antibiotic Resistance Patterns of Bacillus cereus Isolated from Egyptian Milk, Milk Powder, and Ice Cream |
| title_full_unstemmed | Virulence Genes and Antibiotic Resistance Patterns of Bacillus cereus Isolated from Egyptian Milk, Milk Powder, and Ice Cream |
| title_short | Virulence Genes and Antibiotic Resistance Patterns of Bacillus cereus Isolated from Egyptian Milk, Milk Powder, and Ice Cream |
| title_sort | virulence genes and antibiotic resistance patterns of bacillus cereus isolated from egyptian milk milk powder and ice cream |
| topic | Bacillus cereus Virulence genes Antibiotic resistance Dairy products |
| url | https://advetresearch.com/index.php/AVR/article/view/1276 |
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