Polymorphisms within RANKL and Osteoprotegerin Genes in Low Bone Mass among Postmenopausal Indonesian Women
Objective:Prior studies have shown that receptor activator of nuclear factor-B ligand (RANKL) and osteoprotegerin (OPG) have an essential role in bone remodeling and osteoporosis. This study aimed to investigate the association between RANKL (TNFSF11) and OPG (TNFRSF11B) genes’ polymorphisms with lo...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Galenos Yayinevi
2019-04-01
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| Series: | Türk Osteoporoz Dergisi |
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http://www.turkosteoporozdergisi.org/archives/archive-detail/article-preview/polymorphisms-within-rankl-and-osteoprotegerin-gen/27381
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| _version_ | 1797917346441986048 |
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| author | Ignatio Rika Haryono Angela Tulaar Herawati Sudoyo Ambrosius Purba Murdani Abdullah Sri Widia Jusman Andri Lubis Ermita Ibrahim Ilyas |
| author_facet | Ignatio Rika Haryono Angela Tulaar Herawati Sudoyo Ambrosius Purba Murdani Abdullah Sri Widia Jusman Andri Lubis Ermita Ibrahim Ilyas |
| author_sort | Ignatio Rika Haryono |
| collection | DOAJ |
| description | Objective:Prior studies have shown that receptor activator of nuclear factor-B ligand (RANKL) and osteoprotegerin (OPG) have an essential role in bone remodeling and osteoporosis. This study aimed to investigate the association between RANKL (TNFSF11) and OPG (TNFRSF11B) genes’ polymorphisms with low bone mineral density (BMD) in Indonesian postmenopausal women.Materials and Methods:Sixty postmenopausal women aged between 50-65 years were eligible. The BMD was measured by using dualenergy X-ray absorptiometry. The genotypes of TNFSF11 and TNFRSF11B were obtained by polymerase chain reaction-restriction fragment length polymorphisms and DNA sequencing methods. Three single nucleotide polymorphisms (SNPs) from TNFSF11 (-290C>T, -643C>T, -693G>C) and from TNFRSF11B (163A>G, 950T>C, 1181G>C) were selected. The association between alleles distribution and BMD was computed using chi-square or Fischer’s exact test. Multiple logistic regression was used to analyze between all SNPs and BMD at bone sites. Significance was set at p<0.05.Results:Most subjects had lower BMD (83.3%). Characteristics of subjects between healthy and low BMD were comparable (all p>0.05). The distribution of genotypes and alleles in TNSSF11 and TNFRSF11B between healthy and low BMD were not significantly different (all p>0.05). There was no association between SNPs of TNFSF11 and TNFRSF11B with BMD at all bone sites (all p>0.05).Conclusion:The present study suggests that TNFSF11 and TNFRSF11B gene polymorphisms are not associated with BMD in Indonesian postmenopausal women aged between 50-65 years old. |
| first_indexed | 2024-04-10T13:11:51Z |
| format | Article |
| id | doaj.art-c5d8a4c881d547198c8cb06068928494 |
| institution | Directory Open Access Journal |
| issn | 2147-2653 2147-2653 |
| language | English |
| last_indexed | 2024-04-10T13:11:51Z |
| publishDate | 2019-04-01 |
| publisher | Galenos Yayinevi |
| record_format | Article |
| series | Türk Osteoporoz Dergisi |
| spelling | doaj.art-c5d8a4c881d547198c8cb060689284942023-02-15T16:12:37ZengGalenos YayineviTürk Osteoporoz Dergisi2147-26532147-26532019-04-01251283410.4274/tod.galenos.2019.5881513049054Polymorphisms within RANKL and Osteoprotegerin Genes in Low Bone Mass among Postmenopausal Indonesian WomenIgnatio Rika Haryono0Angela Tulaar1Herawati Sudoyo2Ambrosius Purba3Murdani Abdullah4Sri Widia Jusman5Andri Lubis6Ermita Ibrahim Ilyas7 Universitas Katolik Indonesia Atma Jaya Faculty of Medicine, Department of Physiology, Jakarta, Indonesia Universitas Indonesia-dr. Cipto Mangunkusumo General Hospital, Clinic of Physic and Rehabilitation, Jakarta, Indonesia Eijkman Institute for Molecular Biology, Jakarta, Indonesia Padjadjaran University Faculty of Medicine, Department of Physiology, Bandung, Indonesia Universitas Indonesia-Dr. Cipto Mangunkusumo General Hospital, Clinic of Internal Medicine Division of Gastroenterology, Jakarta, Indonesia Universitas Indonesia Faculty of Medicine, Department of Biochemistry and Molecular Biology, Jakarta, Indonesia Universitas Indonesia-Dr. Cipto Mangunkusumo General Hospital, Clinic of Orthopaedic and Traumatology, Jakarta, Indonesia Universitas Indonesia Faculty of Medicine, Department of Medical Physiology, Jakarta, Indonesia Objective:Prior studies have shown that receptor activator of nuclear factor-B ligand (RANKL) and osteoprotegerin (OPG) have an essential role in bone remodeling and osteoporosis. This study aimed to investigate the association between RANKL (TNFSF11) and OPG (TNFRSF11B) genes’ polymorphisms with low bone mineral density (BMD) in Indonesian postmenopausal women.Materials and Methods:Sixty postmenopausal women aged between 50-65 years were eligible. The BMD was measured by using dualenergy X-ray absorptiometry. The genotypes of TNFSF11 and TNFRSF11B were obtained by polymerase chain reaction-restriction fragment length polymorphisms and DNA sequencing methods. Three single nucleotide polymorphisms (SNPs) from TNFSF11 (-290C>T, -643C>T, -693G>C) and from TNFRSF11B (163A>G, 950T>C, 1181G>C) were selected. The association between alleles distribution and BMD was computed using chi-square or Fischer’s exact test. Multiple logistic regression was used to analyze between all SNPs and BMD at bone sites. Significance was set at p<0.05.Results:Most subjects had lower BMD (83.3%). Characteristics of subjects between healthy and low BMD were comparable (all p>0.05). The distribution of genotypes and alleles in TNSSF11 and TNFRSF11B between healthy and low BMD were not significantly different (all p>0.05). There was no association between SNPs of TNFSF11 and TNFRSF11B with BMD at all bone sites (all p>0.05).Conclusion:The present study suggests that TNFSF11 and TNFRSF11B gene polymorphisms are not associated with BMD in Indonesian postmenopausal women aged between 50-65 years old. http://www.turkosteoporozdergisi.org/archives/archive-detail/article-preview/polymorphisms-within-rankl-and-osteoprotegerin-gen/27381 Bone mineral densityRANKL geneOPG genepolymorphismpostmenopause |
| spellingShingle | Ignatio Rika Haryono Angela Tulaar Herawati Sudoyo Ambrosius Purba Murdani Abdullah Sri Widia Jusman Andri Lubis Ermita Ibrahim Ilyas Polymorphisms within RANKL and Osteoprotegerin Genes in Low Bone Mass among Postmenopausal Indonesian Women Türk Osteoporoz Dergisi Bone mineral density RANKL gene OPG gene polymorphism postmenopause |
| title | Polymorphisms within RANKL and Osteoprotegerin Genes in Low Bone Mass among Postmenopausal Indonesian Women |
| title_full | Polymorphisms within RANKL and Osteoprotegerin Genes in Low Bone Mass among Postmenopausal Indonesian Women |
| title_fullStr | Polymorphisms within RANKL and Osteoprotegerin Genes in Low Bone Mass among Postmenopausal Indonesian Women |
| title_full_unstemmed | Polymorphisms within RANKL and Osteoprotegerin Genes in Low Bone Mass among Postmenopausal Indonesian Women |
| title_short | Polymorphisms within RANKL and Osteoprotegerin Genes in Low Bone Mass among Postmenopausal Indonesian Women |
| title_sort | polymorphisms within rankl and osteoprotegerin genes in low bone mass among postmenopausal indonesian women |
| topic | Bone mineral density RANKL gene OPG gene polymorphism postmenopause |
| url |
http://www.turkosteoporozdergisi.org/archives/archive-detail/article-preview/polymorphisms-within-rankl-and-osteoprotegerin-gen/27381
|
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