Structured Illumination Microscopy of Mitochondrial in Mouse Hepatocytes with an Improved Image Reconstruction Algorithm
In this paper, a structured illumination microscopy (SIM) image reconstruction algorithm combined with notch function (N-SIM) is proposed. This method suppresses the defocus signal in the imaging process by processing the low-frequency signal of the image. The existing super-resolution image reconst...
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| Format: | Article |
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MDPI AG
2023-03-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/14/3/642 |
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| author | Kai Hu Xuejuan Hu Ting He Jingxin Liu Shiqian Liu Jiaming Zhang Yadan Tan Xiaokun Yang Hengliang Wang Yifei Liang Jianze Ye |
| author_facet | Kai Hu Xuejuan Hu Ting He Jingxin Liu Shiqian Liu Jiaming Zhang Yadan Tan Xiaokun Yang Hengliang Wang Yifei Liang Jianze Ye |
| author_sort | Kai Hu |
| collection | DOAJ |
| description | In this paper, a structured illumination microscopy (SIM) image reconstruction algorithm combined with notch function (N-SIM) is proposed. This method suppresses the defocus signal in the imaging process by processing the low-frequency signal of the image. The existing super-resolution image reconstruction algorithm produces streak artifacts caused by defocus signal. The experimental results show that the algorithm proposed in our study can well suppress the streak artifacts caused by defocused signals during the imaging process without losing the effective information of the image. The image reconstruction algorithm is used to analyze the mouse hepatocytes, and the image processing tool developed by MATLAB is applied to identify, detect and count the reconstructed images of mitochondria and lipid droplets, respectively. It is found that the mitochondrial activity in oxidative stress induced growth inhibitor 1 (OSGIN1) overexpressed mouse hepatocytes is higher than that in normal cells, and the interaction with lipid droplets is more obvious. This paper provides a reliable subcellular observation platform, which is very meaningful for biomedical work. |
| first_indexed | 2024-03-11T06:09:39Z |
| format | Article |
| id | doaj.art-c5db14837ae742c9a765521d1506c906 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-11T06:09:39Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-c5db14837ae742c9a765521d1506c9062023-11-17T12:43:39ZengMDPI AGMicromachines2072-666X2023-03-0114364210.3390/mi14030642Structured Illumination Microscopy of Mitochondrial in Mouse Hepatocytes with an Improved Image Reconstruction AlgorithmKai Hu0Xuejuan Hu1Ting He2Jingxin Liu3Shiqian Liu4Jiaming Zhang5Yadan Tan6Xiaokun Yang7Hengliang Wang8Yifei Liang9Jianze Ye10Sino-German College of Intelligent Manufacturing, Shenzhen Technology University, Shenzhen 518118, ChinaSino-German College of Intelligent Manufacturing, Shenzhen Technology University, Shenzhen 518118, ChinaSino-German College of Intelligent Manufacturing, Shenzhen Technology University, Shenzhen 518118, ChinaCollege of Pharmacy, Shenzhen Technology University, Shenzhen 518118, ChinaLaboratory of Advanced Optical Precision Manufacturing Technology of Guangdong Provincial Higher Education Institute, Shenzhen Technology University, Shenzhen 518118, ChinaSino-German College of Intelligent Manufacturing, Shenzhen Technology University, Shenzhen 518118, ChinaSino-German College of Intelligent Manufacturing, Shenzhen Technology University, Shenzhen 518118, ChinaSino-German College of Intelligent Manufacturing, Shenzhen Technology University, Shenzhen 518118, ChinaSino-German College of Intelligent Manufacturing, Shenzhen Technology University, Shenzhen 518118, ChinaSino-German College of Intelligent Manufacturing, Shenzhen Technology University, Shenzhen 518118, ChinaSino-German College of Intelligent Manufacturing, Shenzhen Technology University, Shenzhen 518118, ChinaIn this paper, a structured illumination microscopy (SIM) image reconstruction algorithm combined with notch function (N-SIM) is proposed. This method suppresses the defocus signal in the imaging process by processing the low-frequency signal of the image. The existing super-resolution image reconstruction algorithm produces streak artifacts caused by defocus signal. The experimental results show that the algorithm proposed in our study can well suppress the streak artifacts caused by defocused signals during the imaging process without losing the effective information of the image. The image reconstruction algorithm is used to analyze the mouse hepatocytes, and the image processing tool developed by MATLAB is applied to identify, detect and count the reconstructed images of mitochondria and lipid droplets, respectively. It is found that the mitochondrial activity in oxidative stress induced growth inhibitor 1 (OSGIN1) overexpressed mouse hepatocytes is higher than that in normal cells, and the interaction with lipid droplets is more obvious. This paper provides a reliable subcellular observation platform, which is very meaningful for biomedical work.https://www.mdpi.com/2072-666X/14/3/642super-resolutionSIMartifactnotch filterhepatocytemitochondria |
| spellingShingle | Kai Hu Xuejuan Hu Ting He Jingxin Liu Shiqian Liu Jiaming Zhang Yadan Tan Xiaokun Yang Hengliang Wang Yifei Liang Jianze Ye Structured Illumination Microscopy of Mitochondrial in Mouse Hepatocytes with an Improved Image Reconstruction Algorithm Micromachines super-resolution SIM artifact notch filter hepatocyte mitochondria |
| title | Structured Illumination Microscopy of Mitochondrial in Mouse Hepatocytes with an Improved Image Reconstruction Algorithm |
| title_full | Structured Illumination Microscopy of Mitochondrial in Mouse Hepatocytes with an Improved Image Reconstruction Algorithm |
| title_fullStr | Structured Illumination Microscopy of Mitochondrial in Mouse Hepatocytes with an Improved Image Reconstruction Algorithm |
| title_full_unstemmed | Structured Illumination Microscopy of Mitochondrial in Mouse Hepatocytes with an Improved Image Reconstruction Algorithm |
| title_short | Structured Illumination Microscopy of Mitochondrial in Mouse Hepatocytes with an Improved Image Reconstruction Algorithm |
| title_sort | structured illumination microscopy of mitochondrial in mouse hepatocytes with an improved image reconstruction algorithm |
| topic | super-resolution SIM artifact notch filter hepatocyte mitochondria |
| url | https://www.mdpi.com/2072-666X/14/3/642 |
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