Vernalization Procedure of Tuberous Roots Affects Growth, Photosynthesis and Metabolic Profile of <i>Ranunculus asiaticus</i> L.
In <i>Ranunculus asiaticus</i> L., vernalization of propagation material is a common practice for the production scheduling of cut flowers, however little is known about the plant physiology and metabolism of this species as affected by cold treatments. We investigated the influence of t...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/3/425 |
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| author | Giovanna Marta Fusco Petronia Carillo Rosalinda Nicastro Giuseppe Carlo Modarelli Carmen Arena Stefania De Pascale Roberta Paradiso |
| author_facet | Giovanna Marta Fusco Petronia Carillo Rosalinda Nicastro Giuseppe Carlo Modarelli Carmen Arena Stefania De Pascale Roberta Paradiso |
| author_sort | Giovanna Marta Fusco |
| collection | DOAJ |
| description | In <i>Ranunculus asiaticus</i> L., vernalization of propagation material is a common practice for the production scheduling of cut flowers, however little is known about the plant physiology and metabolism of this species as affected by cold treatments. We investigated the influence of two hybrids, MBO and MDR, and three preparation procedures of tuberous roots, only rehydration (control, C), and rehydration plus vernalization at 3.5 °C for 2 weeks (V2) and for 4 weeks (V4), on plant growth and flowering, leaf photosynthesis, and leaf metabolic profile in plants grown in pot in a cold greenhouse. Net photosynthesis (NP) was higher in MDR than in MBO. In the two genotypes, the NP did not change in V2 and increased in V4 compared to C in MBO, while was unaffected by vernalization in MDR. Quantum yield of PSII electron transport (ΦPSII), linear electron transport rate (ETR) and non-photochemical quenching (NPQ) did not differ in the two hybrids, whereas maximal PSII photochemical efficiency (Fv/Fm) was higher in MBO than in MDR. Fluorescence indexes were unaffected by the preparation procedure, except for ETR, which decreased in V2 compared to C and V4 in MDR. A significant interaction between genotype and preparation procedure was found in plant leaf area, which was reduced only in V4 in MBO, while decreased in both the vernalization procedures in MDR. In Control plants, flowering started in 65 days in MBO and 69 days in MDR. Compared to controls, both the vernalization treatments anticipated flowering in MDR, while they were detrimental or only slightly efficient in promoting flowering in MBO. Vernalization always reduced the quality of flower stems in both the hybrids. |
| first_indexed | 2024-03-11T09:30:48Z |
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| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T09:30:48Z |
| publishDate | 2023-01-01 |
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| record_format | Article |
| series | Plants |
| spelling | doaj.art-c5dcbb00a3234187970aaff42f0bd0052023-11-16T17:42:09ZengMDPI AGPlants2223-77472023-01-0112342510.3390/plants12030425Vernalization Procedure of Tuberous Roots Affects Growth, Photosynthesis and Metabolic Profile of <i>Ranunculus asiaticus</i> L.Giovanna Marta Fusco0Petronia Carillo1Rosalinda Nicastro2Giuseppe Carlo Modarelli3Carmen Arena4Stefania De Pascale5Roberta Paradiso6Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania Luigi Vanvitelli, 81100 Caserta, ItalyDepartment of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania Luigi Vanvitelli, 81100 Caserta, ItalyDepartment of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania Luigi Vanvitelli, 81100 Caserta, ItalyDepartment of Agricultural Sciences, University of Naples Federico II, 80055 Naples, ItalyDepartment of Biology, University of Naples Federico II, 80126 Naples, ItalyDepartment of Agricultural Sciences, University of Naples Federico II, 80055 Naples, ItalyDepartment of Agricultural Sciences, University of Naples Federico II, 80055 Naples, ItalyIn <i>Ranunculus asiaticus</i> L., vernalization of propagation material is a common practice for the production scheduling of cut flowers, however little is known about the plant physiology and metabolism of this species as affected by cold treatments. We investigated the influence of two hybrids, MBO and MDR, and three preparation procedures of tuberous roots, only rehydration (control, C), and rehydration plus vernalization at 3.5 °C for 2 weeks (V2) and for 4 weeks (V4), on plant growth and flowering, leaf photosynthesis, and leaf metabolic profile in plants grown in pot in a cold greenhouse. Net photosynthesis (NP) was higher in MDR than in MBO. In the two genotypes, the NP did not change in V2 and increased in V4 compared to C in MBO, while was unaffected by vernalization in MDR. Quantum yield of PSII electron transport (ΦPSII), linear electron transport rate (ETR) and non-photochemical quenching (NPQ) did not differ in the two hybrids, whereas maximal PSII photochemical efficiency (Fv/Fm) was higher in MBO than in MDR. Fluorescence indexes were unaffected by the preparation procedure, except for ETR, which decreased in V2 compared to C and V4 in MDR. A significant interaction between genotype and preparation procedure was found in plant leaf area, which was reduced only in V4 in MBO, while decreased in both the vernalization procedures in MDR. In Control plants, flowering started in 65 days in MBO and 69 days in MDR. Compared to controls, both the vernalization treatments anticipated flowering in MDR, while they were detrimental or only slightly efficient in promoting flowering in MBO. Vernalization always reduced the quality of flower stems in both the hybrids.https://www.mdpi.com/2223-7747/12/3/425geophytescold requirementphotosynthetic pigmentscarbohydratesamino acidsproteins |
| spellingShingle | Giovanna Marta Fusco Petronia Carillo Rosalinda Nicastro Giuseppe Carlo Modarelli Carmen Arena Stefania De Pascale Roberta Paradiso Vernalization Procedure of Tuberous Roots Affects Growth, Photosynthesis and Metabolic Profile of <i>Ranunculus asiaticus</i> L. Plants geophytes cold requirement photosynthetic pigments carbohydrates amino acids proteins |
| title | Vernalization Procedure of Tuberous Roots Affects Growth, Photosynthesis and Metabolic Profile of <i>Ranunculus asiaticus</i> L. |
| title_full | Vernalization Procedure of Tuberous Roots Affects Growth, Photosynthesis and Metabolic Profile of <i>Ranunculus asiaticus</i> L. |
| title_fullStr | Vernalization Procedure of Tuberous Roots Affects Growth, Photosynthesis and Metabolic Profile of <i>Ranunculus asiaticus</i> L. |
| title_full_unstemmed | Vernalization Procedure of Tuberous Roots Affects Growth, Photosynthesis and Metabolic Profile of <i>Ranunculus asiaticus</i> L. |
| title_short | Vernalization Procedure of Tuberous Roots Affects Growth, Photosynthesis and Metabolic Profile of <i>Ranunculus asiaticus</i> L. |
| title_sort | vernalization procedure of tuberous roots affects growth photosynthesis and metabolic profile of i ranunculus asiaticus i l |
| topic | geophytes cold requirement photosynthetic pigments carbohydrates amino acids proteins |
| url | https://www.mdpi.com/2223-7747/12/3/425 |
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