LncRNA ELFN1-AS1 Promotes Retinoblastoma Growth and Invasion via Regulating miR-4270/SBK1 Axis
Wanguo Feng,1 Ruixi Zhu,2 Junlong Ma,3 Han Song2 1Department of Refractive Surgery, Dalian Aier Eye Hospital, Dalian, 116092, People’s Republic of China; 2Department of Ophthalmology, Heilongjiang Provincial Hospital, Harbin Institute of Technology, Harbin, 150036, People’s Repub...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2021-02-01
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| Series: | Cancer Management and Research |
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| Online Access: | https://www.dovepress.com/lncrna-elfn1-as1-promotes-retinoblastoma-growth-and-invasion-via-regul-peer-reviewed-article-CMAR |
| _version_ | 1819296216284921856 |
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| author | Feng W Zhu R Ma J Song H |
| author_facet | Feng W Zhu R Ma J Song H |
| author_sort | Feng W |
| collection | DOAJ |
| description | Wanguo Feng,1 Ruixi Zhu,2 Junlong Ma,3 Han Song2 1Department of Refractive Surgery, Dalian Aier Eye Hospital, Dalian, 116092, People’s Republic of China; 2Department of Ophthalmology, Heilongjiang Provincial Hospital, Harbin Institute of Technology, Harbin, 150036, People’s Republic of China; 3Department of Ophthalmology, Dalian University Affiliated Xinhua Hospital, Dalian, 116021, People’s Republic of ChinaCorrespondence: Han SongDepartment of Ophthalmology, Heilongjiang Provincial Hospital, Harbin Institute of Technology, Harbin, 150036, People’s Republic of ChinaEmail songhan00111@163.comBackground: Long noncoding RNA (lncRNA) has been reported to play important roles in tumor initiation. However, how lncRNA ELFN1-AS1 affects retinoblastoma development remains unclear. Thus, we sought to elucidate its functions in retinoblastoma progression.Methods: ELFN1-AS1 expression was measured in retinoblastoma tissues and normal tissues by qRT-PCR. CCK8, colony formation and Transwell assay were carried out to investigate the effects of ELFN1-AS1 knockdown on cell malignant behaviors. Bioinformatics analyses were performed to predict the relationship among ELFN1-AS1, miR-4270 and SBK1.Results: ELFN1-AS1 was highly expressed in retinoblastoma tissues and cell lines. ELFN1-AS1 was positively correlated with retinoblastoma progression and prognosis. ELFN1-AS1 knockdown curtailed retinoblastoma proliferation, migration and invasion. ELFN1-AS1 was the competing endogenous RNA for miR-4270 and promoted SBK1expression.Conclusion: Altogether, our findings demonstrated that ELFN1-AS1 promotes retinoblastoma progression through mediating miR-4270/SBK1 axis and might be a promising therapeutic target.Keywords: ELFN1-AS1, miR-4270, SBK1, retinoblastoma, progression |
| first_indexed | 2024-12-24T04:54:35Z |
| format | Article |
| id | doaj.art-c5de332bd0084583a718c538007a32a9 |
| institution | Directory Open Access Journal |
| issn | 1179-1322 |
| language | English |
| last_indexed | 2024-12-24T04:54:35Z |
| publishDate | 2021-02-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Cancer Management and Research |
| spelling | doaj.art-c5de332bd0084583a718c538007a32a92022-12-21T17:14:25ZengDove Medical PressCancer Management and Research1179-13222021-02-01Volume 131067107361821LncRNA ELFN1-AS1 Promotes Retinoblastoma Growth and Invasion via Regulating miR-4270/SBK1 AxisFeng WZhu RMa JSong HWanguo Feng,1 Ruixi Zhu,2 Junlong Ma,3 Han Song2 1Department of Refractive Surgery, Dalian Aier Eye Hospital, Dalian, 116092, People’s Republic of China; 2Department of Ophthalmology, Heilongjiang Provincial Hospital, Harbin Institute of Technology, Harbin, 150036, People’s Republic of China; 3Department of Ophthalmology, Dalian University Affiliated Xinhua Hospital, Dalian, 116021, People’s Republic of ChinaCorrespondence: Han SongDepartment of Ophthalmology, Heilongjiang Provincial Hospital, Harbin Institute of Technology, Harbin, 150036, People’s Republic of ChinaEmail songhan00111@163.comBackground: Long noncoding RNA (lncRNA) has been reported to play important roles in tumor initiation. However, how lncRNA ELFN1-AS1 affects retinoblastoma development remains unclear. Thus, we sought to elucidate its functions in retinoblastoma progression.Methods: ELFN1-AS1 expression was measured in retinoblastoma tissues and normal tissues by qRT-PCR. CCK8, colony formation and Transwell assay were carried out to investigate the effects of ELFN1-AS1 knockdown on cell malignant behaviors. Bioinformatics analyses were performed to predict the relationship among ELFN1-AS1, miR-4270 and SBK1.Results: ELFN1-AS1 was highly expressed in retinoblastoma tissues and cell lines. ELFN1-AS1 was positively correlated with retinoblastoma progression and prognosis. ELFN1-AS1 knockdown curtailed retinoblastoma proliferation, migration and invasion. ELFN1-AS1 was the competing endogenous RNA for miR-4270 and promoted SBK1expression.Conclusion: Altogether, our findings demonstrated that ELFN1-AS1 promotes retinoblastoma progression through mediating miR-4270/SBK1 axis and might be a promising therapeutic target.Keywords: ELFN1-AS1, miR-4270, SBK1, retinoblastoma, progressionhttps://www.dovepress.com/lncrna-elfn1-as1-promotes-retinoblastoma-growth-and-invasion-via-regul-peer-reviewed-article-CMARelfn1-as1mir-4270sbk1retinoblastomaprogression |
| spellingShingle | Feng W Zhu R Ma J Song H LncRNA ELFN1-AS1 Promotes Retinoblastoma Growth and Invasion via Regulating miR-4270/SBK1 Axis Cancer Management and Research elfn1-as1 mir-4270 sbk1 retinoblastoma progression |
| title | LncRNA ELFN1-AS1 Promotes Retinoblastoma Growth and Invasion via Regulating miR-4270/SBK1 Axis |
| title_full | LncRNA ELFN1-AS1 Promotes Retinoblastoma Growth and Invasion via Regulating miR-4270/SBK1 Axis |
| title_fullStr | LncRNA ELFN1-AS1 Promotes Retinoblastoma Growth and Invasion via Regulating miR-4270/SBK1 Axis |
| title_full_unstemmed | LncRNA ELFN1-AS1 Promotes Retinoblastoma Growth and Invasion via Regulating miR-4270/SBK1 Axis |
| title_short | LncRNA ELFN1-AS1 Promotes Retinoblastoma Growth and Invasion via Regulating miR-4270/SBK1 Axis |
| title_sort | lncrna elfn1 as1 promotes retinoblastoma growth and invasion via regulating mir 4270 sbk1 axis |
| topic | elfn1-as1 mir-4270 sbk1 retinoblastoma progression |
| url | https://www.dovepress.com/lncrna-elfn1-as1-promotes-retinoblastoma-growth-and-invasion-via-regul-peer-reviewed-article-CMAR |
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