Molecular Epidemiological Evaluation of Acinetobacter baumannii Isolates Isolated As the Agent of Hospital Infections in Türkiye
Introduction: Acinetobacter baumannii is a major nosocomial pathogen which can cause infections with high morbidity and mortality in hospitalized patients. The aim of this study was to evaluate antibiotic susceptibility of nosocomial Acinetobacter baumannii isolates and to determine, by using the re...
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| Format: | Article |
| Language: | English |
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Bilimsel Tip Yayinevi
2022-06-01
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| Series: | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| Subjects: | |
| Online Access: | http://floradergisi.org/managete/fu_folder/2022-02/227-240%20Arzu%20Kayis.pdf |
| _version_ | 1797907733066809344 |
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| author | Arzu KAYIŞ Murat ARAL |
| author_facet | Arzu KAYIŞ Murat ARAL |
| author_sort | Arzu KAYIŞ |
| collection | DOAJ |
| description | Introduction: Acinetobacter baumannii is a major nosocomial pathogen which can cause infections with high morbidity and mortality in hospitalized patients. The aim of this study was to evaluate antibiotic susceptibility of nosocomial Acinetobacter baumannii isolates and to determine, by using the rep-PCR method, the clonal relationship between these isolates.
Materials and Methods: A total of 70 nosocomial Acinetobacter baumannii isolates identified by BD Phoenix automated microbiology system and isolated by standard bacteriologic methods from various clinical samples that was sent to Medical Microbiology Laboratory of a university research and practice hospital at the period of June 2014-October 2016 were used in this study. The sensitivity of Acinetobacter baumannii isolates to different antibiotics was determined by BD Phoenix automated microbiology system.
Results: Antibiotic resistance rates obtained from isolates of Acinetobacter baumannii by BD Phoenix method; ertapenem 100%; amoxicillin-clavulanate, ampicillin, ceftriaxon, cefuroxime 98.6%; aztreonam, ceftazidime, ciprofloxacin, imipenem, piperacillin and piperacillin-tazobactam 97.1%; cefepime, gentamicin, meropenem and netilmicine 95.7%; amikacine 91.4%; trimethoprim-sulfamethoxazole 88.5%; tigecycline 45.7%; colistine 4.3% respectively. As a result of the clonal correlation analysis with Rep-PCR; 10 clones were identified, one being the main clone. The similarity rate between isolates was 95.8%. Clone 1 was found to be the dominant type. The time interval between the first and last isolate was eighteen months in dominant clone.
Conclusion: It was concluded that Acinetobacter baumannii isolates were scattered as a result of cross transmission and patient transfer among clinics in the hospital. The clonal relationship of resistant isolates in the hospital environment once again showed the importance of infection control measures |
| first_indexed | 2024-04-10T10:41:29Z |
| format | Article |
| id | doaj.art-c5e02f808a8145db92c226d5c54595dc |
| institution | Directory Open Access Journal |
| issn | 1300-932X 1308-5115 |
| language | English |
| last_indexed | 2024-04-10T10:41:29Z |
| publishDate | 2022-06-01 |
| publisher | Bilimsel Tip Yayinevi |
| record_format | Article |
| series | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| spelling | doaj.art-c5e02f808a8145db92c226d5c54595dc2023-02-15T16:20:34ZengBilimsel Tip YayineviFlora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi1300-932X1308-51152022-06-0127222724010.5578/flora.20229806Molecular Epidemiological Evaluation of Acinetobacter baumannii Isolates Isolated As the Agent of Hospital Infections in TürkiyeArzu KAYIŞ0https://orcid.org/0000-0002-0061-1907Murat ARAL1https://orcid.org/0000-0002-3576-4380Program of Medical Laboratory Techniques, Kahramanmaraş Sütçü İmam University Vocational School of Health Services, Kahramanmaraş, TürkiyeClinic of Medical Microbiology, Kahramanmaraş Sütçü İmam University Research and Practice Hospital, Kahramanmaraş, TürkiyeIntroduction: Acinetobacter baumannii is a major nosocomial pathogen which can cause infections with high morbidity and mortality in hospitalized patients. The aim of this study was to evaluate antibiotic susceptibility of nosocomial Acinetobacter baumannii isolates and to determine, by using the rep-PCR method, the clonal relationship between these isolates. Materials and Methods: A total of 70 nosocomial Acinetobacter baumannii isolates identified by BD Phoenix automated microbiology system and isolated by standard bacteriologic methods from various clinical samples that was sent to Medical Microbiology Laboratory of a university research and practice hospital at the period of June 2014-October 2016 were used in this study. The sensitivity of Acinetobacter baumannii isolates to different antibiotics was determined by BD Phoenix automated microbiology system. Results: Antibiotic resistance rates obtained from isolates of Acinetobacter baumannii by BD Phoenix method; ertapenem 100%; amoxicillin-clavulanate, ampicillin, ceftriaxon, cefuroxime 98.6%; aztreonam, ceftazidime, ciprofloxacin, imipenem, piperacillin and piperacillin-tazobactam 97.1%; cefepime, gentamicin, meropenem and netilmicine 95.7%; amikacine 91.4%; trimethoprim-sulfamethoxazole 88.5%; tigecycline 45.7%; colistine 4.3% respectively. As a result of the clonal correlation analysis with Rep-PCR; 10 clones were identified, one being the main clone. The similarity rate between isolates was 95.8%. Clone 1 was found to be the dominant type. The time interval between the first and last isolate was eighteen months in dominant clone. Conclusion: It was concluded that Acinetobacter baumannii isolates were scattered as a result of cross transmission and patient transfer among clinics in the hospital. The clonal relationship of resistant isolates in the hospital environment once again showed the importance of infection control measureshttp://floradergisi.org/managete/fu_folder/2022-02/227-240%20Arzu%20Kayis.pdfacinetobacter baumanniiantibiotic resistanceclonal relationshiprep-pcrnosocomial infection |
| spellingShingle | Arzu KAYIŞ Murat ARAL Molecular Epidemiological Evaluation of Acinetobacter baumannii Isolates Isolated As the Agent of Hospital Infections in Türkiye Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi acinetobacter baumannii antibiotic resistance clonal relationship rep-pcr nosocomial infection |
| title | Molecular Epidemiological Evaluation of Acinetobacter baumannii Isolates Isolated As the Agent of Hospital Infections in Türkiye |
| title_full | Molecular Epidemiological Evaluation of Acinetobacter baumannii Isolates Isolated As the Agent of Hospital Infections in Türkiye |
| title_fullStr | Molecular Epidemiological Evaluation of Acinetobacter baumannii Isolates Isolated As the Agent of Hospital Infections in Türkiye |
| title_full_unstemmed | Molecular Epidemiological Evaluation of Acinetobacter baumannii Isolates Isolated As the Agent of Hospital Infections in Türkiye |
| title_short | Molecular Epidemiological Evaluation of Acinetobacter baumannii Isolates Isolated As the Agent of Hospital Infections in Türkiye |
| title_sort | molecular epidemiological evaluation of acinetobacter baumannii isolates isolated as the agent of hospital infections in turkiye |
| topic | acinetobacter baumannii antibiotic resistance clonal relationship rep-pcr nosocomial infection |
| url | http://floradergisi.org/managete/fu_folder/2022-02/227-240%20Arzu%20Kayis.pdf |
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