Vitamin D, serum 25(OH)D, LL-37 and polymorphisms in a Canadian First Nation population with endemic tuberculosis
Background: Canadian First Nation populations have experienced endemic and epidemic tuberculosis (TB) for decades. Vitamin D–mediated induction of the host defence peptide LL-37 is known to enhance control of pathogens such as Mycobacterium tuberculosis. Objective: Evaluate associations between seru...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2015-08-01
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| Series: | International Journal of Circumpolar Health |
| Subjects: | |
| Online Access: | http://www.circumpolarhealthjournal.net/index.php/ijch/article/view/28952/pdf_40 |
| _version_ | 1818136873127641088 |
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| author | Linda Larcombe Neeloffer Mookherjee Joyce Slater Caroline Slivinski Joe Dantouze Matthew Singer Chris Whaley Lizette Denechezhe Sara Matyas Kate Decter Emily Turner-Brannen Clare Ramsey Peter Nickerson Pamela Orr |
| author_facet | Linda Larcombe Neeloffer Mookherjee Joyce Slater Caroline Slivinski Joe Dantouze Matthew Singer Chris Whaley Lizette Denechezhe Sara Matyas Kate Decter Emily Turner-Brannen Clare Ramsey Peter Nickerson Pamela Orr |
| author_sort | Linda Larcombe |
| collection | DOAJ |
| description | Background: Canadian First Nation populations have experienced endemic and epidemic tuberculosis (TB) for decades. Vitamin D–mediated induction of the host defence peptide LL-37 is known to enhance control of pathogens such as Mycobacterium tuberculosis. Objective: Evaluate associations between serum levels of 25-hydroxy vitamin D (25(OH)D) and LL-37, in adult Dene First Nation participants (N = 34) and assess correlations with single nucleotide polymorphisms (SNPs) in the vitamin D receptor (VDR) and vitamin D binding protein (VDBP). Design: Venous blood was collected from all participants at baseline (winter and summer) and in conjunction with taking vitamin D supplements (1,000 IU/day) (winter and summer). Samples were analysed using ELISA for concentrations of vitamin D and LL-37, and SNPs in the VDR and VDBP regions were genotyped. Results: Circulating levels of 25(OH)D were not altered by vitamin D supplementation, but LL-37 levels were significantly decreased. VDBP and VDR SNPs did not correlate with serum concentrations of 25(OH)D, but LL-37 levels significantly decreased in individuals with VDBP D432E T/G and T/T, and with VDR SNP Bsm1 T/T genotypes. Conclusions: Our findings suggest that vitamin D supplementation may not be beneficial as an intervention to boost innate immune resistance to M. tuberculosis in the Dene population. |
| first_indexed | 2024-12-11T09:47:19Z |
| format | Article |
| id | doaj.art-c5e41148987f4bf1b0433ee207d968ca |
| institution | Directory Open Access Journal |
| issn | 2242-3982 |
| language | English |
| last_indexed | 2024-12-11T09:47:19Z |
| publishDate | 2015-08-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | International Journal of Circumpolar Health |
| spelling | doaj.art-c5e41148987f4bf1b0433ee207d968ca2022-12-22T01:12:32ZengTaylor & Francis GroupInternational Journal of Circumpolar Health2242-39822015-08-017401910.3402/ijch.v74.2895228952Vitamin D, serum 25(OH)D, LL-37 and polymorphisms in a Canadian First Nation population with endemic tuberculosisLinda Larcombe0Neeloffer Mookherjee1Joyce Slater2Caroline Slivinski3Joe Dantouze4Matthew Singer5Chris Whaley6Lizette Denechezhe7Sara Matyas8Kate Decter9Emily Turner-Brannen10Clare Ramsey11Peter Nickerson12Pamela Orr13 Department of Internal Medicine, University of Manitoba, Winnipeg, MB, Canada Department of Internal Medicine, University of Manitoba, Winnipeg, MB, Canada Department of Human Nutritional Sciences, University of Manitoba, Winnipeg, MB, Canada Department of Medical Microbiology, University of Manitoba, Winnipeg, MB, Canada Northlands Denesuline First Nation, Lac Brochet, MB, Canada Department of Medical Microbiology, University of Manitoba, Winnipeg, MB, Canada Department of Medical Microbiology, University of Manitoba, Winnipeg, MB, Canada Northlands Denesuline First Nation, Lac Brochet, MB, Canada Department of Internal Medicine, University of Manitoba, Winnipeg, MB, Canada Department of Internal Medicine, University of Manitoba, Winnipeg, MB, Canada Department of Internal Medicine, University of Manitoba, Winnipeg, MB, Canada Department of Internal Medicine, University of Manitoba, Winnipeg, MB, Canada Department of Internal Medicine, University of Manitoba, Winnipeg, MB, Canada Department of Internal Medicine, University of Manitoba, Winnipeg, MB, CanadaBackground: Canadian First Nation populations have experienced endemic and epidemic tuberculosis (TB) for decades. Vitamin D–mediated induction of the host defence peptide LL-37 is known to enhance control of pathogens such as Mycobacterium tuberculosis. Objective: Evaluate associations between serum levels of 25-hydroxy vitamin D (25(OH)D) and LL-37, in adult Dene First Nation participants (N = 34) and assess correlations with single nucleotide polymorphisms (SNPs) in the vitamin D receptor (VDR) and vitamin D binding protein (VDBP). Design: Venous blood was collected from all participants at baseline (winter and summer) and in conjunction with taking vitamin D supplements (1,000 IU/day) (winter and summer). Samples were analysed using ELISA for concentrations of vitamin D and LL-37, and SNPs in the VDR and VDBP regions were genotyped. Results: Circulating levels of 25(OH)D were not altered by vitamin D supplementation, but LL-37 levels were significantly decreased. VDBP and VDR SNPs did not correlate with serum concentrations of 25(OH)D, but LL-37 levels significantly decreased in individuals with VDBP D432E T/G and T/T, and with VDR SNP Bsm1 T/T genotypes. Conclusions: Our findings suggest that vitamin D supplementation may not be beneficial as an intervention to boost innate immune resistance to M. tuberculosis in the Dene population.http://www.circumpolarhealthjournal.net/index.php/ijch/article/view/28952/pdf_40vitamin Dindigenousgeneticscathelicidin (LL-37)tuberculosis |
| spellingShingle | Linda Larcombe Neeloffer Mookherjee Joyce Slater Caroline Slivinski Joe Dantouze Matthew Singer Chris Whaley Lizette Denechezhe Sara Matyas Kate Decter Emily Turner-Brannen Clare Ramsey Peter Nickerson Pamela Orr Vitamin D, serum 25(OH)D, LL-37 and polymorphisms in a Canadian First Nation population with endemic tuberculosis International Journal of Circumpolar Health vitamin D indigenous genetics cathelicidin (LL-37) tuberculosis |
| title | Vitamin D, serum 25(OH)D, LL-37 and polymorphisms in a Canadian First Nation population with endemic tuberculosis |
| title_full | Vitamin D, serum 25(OH)D, LL-37 and polymorphisms in a Canadian First Nation population with endemic tuberculosis |
| title_fullStr | Vitamin D, serum 25(OH)D, LL-37 and polymorphisms in a Canadian First Nation population with endemic tuberculosis |
| title_full_unstemmed | Vitamin D, serum 25(OH)D, LL-37 and polymorphisms in a Canadian First Nation population with endemic tuberculosis |
| title_short | Vitamin D, serum 25(OH)D, LL-37 and polymorphisms in a Canadian First Nation population with endemic tuberculosis |
| title_sort | vitamin d serum 25 oh d ll 37 and polymorphisms in a canadian first nation population with endemic tuberculosis |
| topic | vitamin D indigenous genetics cathelicidin (LL-37) tuberculosis |
| url | http://www.circumpolarhealthjournal.net/index.php/ijch/article/view/28952/pdf_40 |
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