Simultaneous use of sodium nitrate and urea as nitrogen sources improves biomass composition of Arthrospira platensis cultivated in a tubular photobioreactor
Arthrospira platensis is widely cultivated in open ponds for industrial purposes. However, high‐protein A. platensis biomass produced in photobioreactors (PBRs) is recommended for pharmaceutical and cosmetic formulations. A. platensis was cultivated in a 3.5 L tubular airlift PBR using both sodium n...
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2016-05-01
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| Series: | Engineering in Life Sciences |
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| Online Access: | https://doi.org/10.1002/elsc.201500051 |
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| author | Ana Lucía Morocho‐Jácome Sunao Sato Laís deLara Capurro Guimarães Camila Knysak Camargo deJesus João Carlos Monteiro deCarvalho |
| author_facet | Ana Lucía Morocho‐Jácome Sunao Sato Laís deLara Capurro Guimarães Camila Knysak Camargo deJesus João Carlos Monteiro deCarvalho |
| author_sort | Ana Lucía Morocho‐Jácome |
| collection | DOAJ |
| description | Arthrospira platensis is widely cultivated in open ponds for industrial purposes. However, high‐protein A. platensis biomass produced in photobioreactors (PBRs) is recommended for pharmaceutical and cosmetic formulations. A. platensis was cultivated in a 3.5 L tubular airlift PBR using both sodium nitrate and urea as nitrogen sources. Sodium nitrate was added from the start of the cultivation using a batch process. Urea was supplied daily at exponentially increasing feeding rate using a fed‐batch process. The simultaneous optimization of the independent variables, namely, total quantity of sodium nitrate (mT1) and total quantity of urea (mT2), led to an optimal condition of mT1 = 15.0 mmol/L and mT2 = 7.5 mmol/L. Maximum biomass concentration (5183 ± 94 mg/L) corresponding to the highest biomass productivity (683 ± 13 mg/L/day) was obtained under such condition. The addition protocol of both nitrogen sources resulted in high productivities of protein (6.2 ± 0.4 mg/L/day) as well as chlorophyll‐a (372.2 ± 7.7 mg/L/day). Such innovative process could be applied in the large‐scale production of A. platensis using tubular PBR for novel applications. |
| first_indexed | 2024-12-23T13:50:19Z |
| format | Article |
| id | doaj.art-c5e654efe9034e11a0fb82d546a131c8 |
| institution | Directory Open Access Journal |
| issn | 1618-0240 1618-2863 |
| language | English |
| last_indexed | 2024-12-23T13:50:19Z |
| publishDate | 2016-05-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Engineering in Life Sciences |
| spelling | doaj.art-c5e654efe9034e11a0fb82d546a131c82022-12-21T17:44:37ZengWiley-VCHEngineering in Life Sciences1618-02401618-28632016-05-0116433834710.1002/elsc.201500051Simultaneous use of sodium nitrate and urea as nitrogen sources improves biomass composition of Arthrospira platensis cultivated in a tubular photobioreactorAna Lucía Morocho‐Jácome0Sunao Sato1Laís deLara Capurro Guimarães2Camila Knysak Camargo deJesus3João Carlos Monteiro deCarvalho4Department of Biochemical and Pharmaceutical Technology Faculty of Pharmaceutical Sciences, University of São Paulo São Paulo BrazilDepartment of Biochemical and Pharmaceutical Technology Faculty of Pharmaceutical Sciences, University of São Paulo São Paulo BrazilDepartment of Biochemical and Pharmaceutical Technology Faculty of Pharmaceutical Sciences, University of São Paulo São Paulo BrazilDepartment of Biochemical and Pharmaceutical Technology Faculty of Pharmaceutical Sciences, University of São Paulo São Paulo BrazilDepartment of Biochemical and Pharmaceutical Technology Faculty of Pharmaceutical Sciences, University of São Paulo São Paulo BrazilArthrospira platensis is widely cultivated in open ponds for industrial purposes. However, high‐protein A. platensis biomass produced in photobioreactors (PBRs) is recommended for pharmaceutical and cosmetic formulations. A. platensis was cultivated in a 3.5 L tubular airlift PBR using both sodium nitrate and urea as nitrogen sources. Sodium nitrate was added from the start of the cultivation using a batch process. Urea was supplied daily at exponentially increasing feeding rate using a fed‐batch process. The simultaneous optimization of the independent variables, namely, total quantity of sodium nitrate (mT1) and total quantity of urea (mT2), led to an optimal condition of mT1 = 15.0 mmol/L and mT2 = 7.5 mmol/L. Maximum biomass concentration (5183 ± 94 mg/L) corresponding to the highest biomass productivity (683 ± 13 mg/L/day) was obtained under such condition. The addition protocol of both nitrogen sources resulted in high productivities of protein (6.2 ± 0.4 mg/L/day) as well as chlorophyll‐a (372.2 ± 7.7 mg/L/day). Such innovative process could be applied in the large‐scale production of A. platensis using tubular PBR for novel applications.https://doi.org/10.1002/elsc.201500051Arthrospira (Spirulina) platensisBiomass compositionSodium nitrateTubular photobioreactorUrea |
| spellingShingle | Ana Lucía Morocho‐Jácome Sunao Sato Laís deLara Capurro Guimarães Camila Knysak Camargo deJesus João Carlos Monteiro deCarvalho Simultaneous use of sodium nitrate and urea as nitrogen sources improves biomass composition of Arthrospira platensis cultivated in a tubular photobioreactor Engineering in Life Sciences Arthrospira (Spirulina) platensis Biomass composition Sodium nitrate Tubular photobioreactor Urea |
| title | Simultaneous use of sodium nitrate and urea as nitrogen sources improves biomass composition of Arthrospira platensis cultivated in a tubular photobioreactor |
| title_full | Simultaneous use of sodium nitrate and urea as nitrogen sources improves biomass composition of Arthrospira platensis cultivated in a tubular photobioreactor |
| title_fullStr | Simultaneous use of sodium nitrate and urea as nitrogen sources improves biomass composition of Arthrospira platensis cultivated in a tubular photobioreactor |
| title_full_unstemmed | Simultaneous use of sodium nitrate and urea as nitrogen sources improves biomass composition of Arthrospira platensis cultivated in a tubular photobioreactor |
| title_short | Simultaneous use of sodium nitrate and urea as nitrogen sources improves biomass composition of Arthrospira platensis cultivated in a tubular photobioreactor |
| title_sort | simultaneous use of sodium nitrate and urea as nitrogen sources improves biomass composition of arthrospira platensis cultivated in a tubular photobioreactor |
| topic | Arthrospira (Spirulina) platensis Biomass composition Sodium nitrate Tubular photobioreactor Urea |
| url | https://doi.org/10.1002/elsc.201500051 |
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