Insulin-like Growth Factor Administration Stimulates Wound Healing on Colonic Anastomosis in Hypoxic Rat Model
Objective: Intestinal anastomosis is among the most commonly performed major abdominal procedures; however, leaks are an ongoing problem. The aim of this study was to investigate the effect of Insulin-like Growth Factor (IGF-1) on colonic healing under hypoxia. Methods: Thirty-two Sprague–Dawley ma...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Springer
2020-06-01
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| Series: | Dr. Sulaiman Al Habib Medical Journal |
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| Online Access: | https://www.atlantis-press.com/article/125941073/view |
| _version_ | 1811229062682640384 |
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| author | Khayal Al-Khayal Alanoud Saad AlWesaimer Maha Abdulla Rehan Ahmad |
| author_facet | Khayal Al-Khayal Alanoud Saad AlWesaimer Maha Abdulla Rehan Ahmad |
| author_sort | Khayal Al-Khayal |
| collection | DOAJ |
| description | Objective: Intestinal anastomosis is among the most commonly performed major abdominal procedures; however, leaks are an ongoing problem. The aim of this study was to investigate the effect of Insulin-like Growth Factor (IGF-1) on colonic healing under hypoxia.
Methods: Thirty-two Sprague–Dawley male rats underwent laparotomy with colonic transection and anastomosis. Rats were divided into four groups—Group A: normoxic (FiO2 21%) with saline [2 mg/kg, Intraperitoneally (IP)] at days 0, 2, 4, and 6; Group B: hypoxic (FiO2 11%) with saline injection; Group C: normoxic with IGF-1 injection at a dose of 2 mg/kg at days 0, 2, 4, and 6; Group D: hypoxic with IGF-1 treatment. On day 7, all animals were sacrificed, and intestine weight and bursting pressure were measured; anastomotic tissues were analyzed for mRNA level of collagen I and Matrix Metalloproteinase-13 (MMP-13) and cytokines analysis by enzyme-linked immunosorbent assay.
Results: There was significant increase in intestinal length in normoxic group treated with IGF-1 as compared to control (129.9 cm vs. 99.38 cm, p < 0.05); the length increased significantly in hypoxic group treated with IGF as compared to hypoxic control (123.1 cm vs. 82.25, p < 0.01). In the normoxic groups, a 6.7% decrease in mean body weight was found; however, the normoxic IGF-1 group sustained a weight gain of 0.5% (p < 0.01). The average weight decreased in the hypoxic control group (28.3%) and in the hypoxic IGF-1 treated group (16.6%, p < 0.05). Anastomotic bursting pressure increased significantly (p < 0.05) after IGF-1 treatment. Collagen I and MMP-13 mRNA expressions increased significantly (p < 0.001) in the IGF-1 treated group. Interleukin-10 and tumor necrosis factor alpha levels decreased under hypoxia with IGF-1 (p < 0.05). Vascular endothelial growth factor (p < 0.001), transforming growth factor beta (p < 0.05), and IGF-1 (p < 0.001) were significantly elevated in response to IGF-1.
Conclusion: IGF-1 stimulates the healing of colonic anastomosis under hypoxia. Factors promoting neoangiogenesis, collagen deposition, and paracrine expression were increased after IGF-1 treatment resulting in wound healing. |
| first_indexed | 2024-04-12T10:06:53Z |
| format | Article |
| id | doaj.art-c5eb80d253e74493a192137c19c3035e |
| institution | Directory Open Access Journal |
| issn | 2590-3349 |
| language | English |
| last_indexed | 2024-04-12T10:06:53Z |
| publishDate | 2020-06-01 |
| publisher | Springer |
| record_format | Article |
| series | Dr. Sulaiman Al Habib Medical Journal |
| spelling | doaj.art-c5eb80d253e74493a192137c19c3035e2022-12-22T03:37:24ZengSpringerDr. Sulaiman Al Habib Medical Journal2590-33492020-06-012210.2991/dsahmj.k.200529.001Insulin-like Growth Factor Administration Stimulates Wound Healing on Colonic Anastomosis in Hypoxic Rat ModelKhayal Al-KhayalAlanoud Saad AlWesaimerMaha AbdullaRehan AhmadObjective: Intestinal anastomosis is among the most commonly performed major abdominal procedures; however, leaks are an ongoing problem. The aim of this study was to investigate the effect of Insulin-like Growth Factor (IGF-1) on colonic healing under hypoxia. Methods: Thirty-two Sprague–Dawley male rats underwent laparotomy with colonic transection and anastomosis. Rats were divided into four groups—Group A: normoxic (FiO2 21%) with saline [2 mg/kg, Intraperitoneally (IP)] at days 0, 2, 4, and 6; Group B: hypoxic (FiO2 11%) with saline injection; Group C: normoxic with IGF-1 injection at a dose of 2 mg/kg at days 0, 2, 4, and 6; Group D: hypoxic with IGF-1 treatment. On day 7, all animals were sacrificed, and intestine weight and bursting pressure were measured; anastomotic tissues were analyzed for mRNA level of collagen I and Matrix Metalloproteinase-13 (MMP-13) and cytokines analysis by enzyme-linked immunosorbent assay. Results: There was significant increase in intestinal length in normoxic group treated with IGF-1 as compared to control (129.9 cm vs. 99.38 cm, p < 0.05); the length increased significantly in hypoxic group treated with IGF as compared to hypoxic control (123.1 cm vs. 82.25, p < 0.01). In the normoxic groups, a 6.7% decrease in mean body weight was found; however, the normoxic IGF-1 group sustained a weight gain of 0.5% (p < 0.01). The average weight decreased in the hypoxic control group (28.3%) and in the hypoxic IGF-1 treated group (16.6%, p < 0.05). Anastomotic bursting pressure increased significantly (p < 0.05) after IGF-1 treatment. Collagen I and MMP-13 mRNA expressions increased significantly (p < 0.001) in the IGF-1 treated group. Interleukin-10 and tumor necrosis factor alpha levels decreased under hypoxia with IGF-1 (p < 0.05). Vascular endothelial growth factor (p < 0.001), transforming growth factor beta (p < 0.05), and IGF-1 (p < 0.001) were significantly elevated in response to IGF-1. Conclusion: IGF-1 stimulates the healing of colonic anastomosis under hypoxia. Factors promoting neoangiogenesis, collagen deposition, and paracrine expression were increased after IGF-1 treatment resulting in wound healing.https://www.atlantis-press.com/article/125941073/viewCytokinegastrointestinal anastomosishypoxiainsulin-like growth factor-1TGF-β |
| spellingShingle | Khayal Al-Khayal Alanoud Saad AlWesaimer Maha Abdulla Rehan Ahmad Insulin-like Growth Factor Administration Stimulates Wound Healing on Colonic Anastomosis in Hypoxic Rat Model Dr. Sulaiman Al Habib Medical Journal Cytokine gastrointestinal anastomosis hypoxia insulin-like growth factor-1 TGF-β |
| title | Insulin-like Growth Factor Administration Stimulates Wound Healing on Colonic Anastomosis in Hypoxic Rat Model |
| title_full | Insulin-like Growth Factor Administration Stimulates Wound Healing on Colonic Anastomosis in Hypoxic Rat Model |
| title_fullStr | Insulin-like Growth Factor Administration Stimulates Wound Healing on Colonic Anastomosis in Hypoxic Rat Model |
| title_full_unstemmed | Insulin-like Growth Factor Administration Stimulates Wound Healing on Colonic Anastomosis in Hypoxic Rat Model |
| title_short | Insulin-like Growth Factor Administration Stimulates Wound Healing on Colonic Anastomosis in Hypoxic Rat Model |
| title_sort | insulin like growth factor administration stimulates wound healing on colonic anastomosis in hypoxic rat model |
| topic | Cytokine gastrointestinal anastomosis hypoxia insulin-like growth factor-1 TGF-β |
| url | https://www.atlantis-press.com/article/125941073/view |
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