Effect of propolis extract on clinical parameters and salivary level of matrix metalloproteinase 8 in periodontitis patients: A randomized controlled clinical trial
Background. Periodontitis is the bacterial-induced inflammation of tooth-supporting structures. Local antibacterial agents are used as adjunctive therapy in the treatment of periodontitis. This study aimed to compare the effect of subgingivally delivered propolis extract (a resin produced by honey b...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tabriz University of Medical Sciences
2021-12-01
|
| Series: | Journal of Advanced Periodontology and Implant Dentistry |
| Subjects: | |
| Online Access: | https://japid.tbzmed.ac.ir/PDF/japid-13-84.pdf |
| _version_ | 1818469499169406976 |
|---|---|
| author | Reyhaneh Eghbali Zarch Mitra Askari Hamidreza boostani Iraj Mirzaii-Dizgah |
| author_facet | Reyhaneh Eghbali Zarch Mitra Askari Hamidreza boostani Iraj Mirzaii-Dizgah |
| author_sort | Reyhaneh Eghbali Zarch |
| collection | DOAJ |
| description | Background. Periodontitis is the bacterial-induced inflammation of tooth-supporting structures. Local antibacterial agents are used as adjunctive therapy in the treatment of periodontitis. This study aimed to compare the effect of subgingivally delivered propolis extract (a resin produced by honey bees) with chlorhexidine (CHX) mouthwash on clinical parameters and salivary levels of matrix metalloproteinase 8 (MMP-8) in periodontitis patients.Methods. Twenty-eight periodontitis patients in stage II or III and grade B, who had deep periodontal pockets (≥4 mm) around at least three non-adjacent teeth, were divided into two groups. In the control group, patients were prescribed 0.2% CHX mouthwash twice a day for two weeks. In the 20% propolis hydroalcoholic group, subgingival irrigation was performed twice a week for two weeks. Clinical parameters were measured at baseline and after two months. Salivary samples were collected from the propolis and control groups at baseline and two months later to assess MMP-8 levels using the enzyme-linked immunosorbent assay. Additionally, salivary samples from 12 periodontally healthy subjects were used to determine the normal levels of MMP-8. The data were analyzed using SPSS. P<0.05 was considered the level of significance.Results. In the healthy group, the mean salivary levels of MMP-8 were significantly lower than that in the control and propolis groups at baseline (P<0.001). The results indicated a significant improvement in clinical parameters (P<0.001) in the propolis group compared to the control group, while MMP-8 levels decreased significantly in both groups (P<0.001).Conclusion. Propolis is recommended as adjunctive therapy for periodontitis patients. Clinical trials registration code: IRCT2016122030475N3. |
| first_indexed | 2024-04-13T21:25:21Z |
| format | Article |
| id | doaj.art-c5ec3e622c0949fdb3fd20f9532779bd |
| institution | Directory Open Access Journal |
| issn | 2645-5390 |
| language | English |
| last_indexed | 2024-04-13T21:25:21Z |
| publishDate | 2021-12-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | Journal of Advanced Periodontology and Implant Dentistry |
| spelling | doaj.art-c5ec3e622c0949fdb3fd20f9532779bd2022-12-22T02:29:21ZengTabriz University of Medical SciencesJournal of Advanced Periodontology and Implant Dentistry2645-53902021-12-01132849010.34172/japid.2021.013japid-1148Effect of propolis extract on clinical parameters and salivary level of matrix metalloproteinase 8 in periodontitis patients: A randomized controlled clinical trialReyhaneh Eghbali Zarch0Mitra Askari1Hamidreza boostani2Iraj Mirzaii-Dizgah3Faculty of Dentistry, Tehran University of Medical Sciences, Tehran, IranOral and Maxillofacial Pathology Department, Faculty of Dentistry, Tehran University of Medical Sciences, International Campus, Tehran, IranPeriodontist, Private Practice, Tehran, IranPhysiology Department, Aja University of Medical Sciences, Tehran, IranBackground. Periodontitis is the bacterial-induced inflammation of tooth-supporting structures. Local antibacterial agents are used as adjunctive therapy in the treatment of periodontitis. This study aimed to compare the effect of subgingivally delivered propolis extract (a resin produced by honey bees) with chlorhexidine (CHX) mouthwash on clinical parameters and salivary levels of matrix metalloproteinase 8 (MMP-8) in periodontitis patients.Methods. Twenty-eight periodontitis patients in stage II or III and grade B, who had deep periodontal pockets (≥4 mm) around at least three non-adjacent teeth, were divided into two groups. In the control group, patients were prescribed 0.2% CHX mouthwash twice a day for two weeks. In the 20% propolis hydroalcoholic group, subgingival irrigation was performed twice a week for two weeks. Clinical parameters were measured at baseline and after two months. Salivary samples were collected from the propolis and control groups at baseline and two months later to assess MMP-8 levels using the enzyme-linked immunosorbent assay. Additionally, salivary samples from 12 periodontally healthy subjects were used to determine the normal levels of MMP-8. The data were analyzed using SPSS. P<0.05 was considered the level of significance.Results. In the healthy group, the mean salivary levels of MMP-8 were significantly lower than that in the control and propolis groups at baseline (P<0.001). The results indicated a significant improvement in clinical parameters (P<0.001) in the propolis group compared to the control group, while MMP-8 levels decreased significantly in both groups (P<0.001).Conclusion. Propolis is recommended as adjunctive therapy for periodontitis patients. Clinical trials registration code: IRCT2016122030475N3.https://japid.tbzmed.ac.ir/PDF/japid-13-84.pdfmatrix metalloproteinase 8periodontitispropolisrandomized controlled trialsaliva |
| spellingShingle | Reyhaneh Eghbali Zarch Mitra Askari Hamidreza boostani Iraj Mirzaii-Dizgah Effect of propolis extract on clinical parameters and salivary level of matrix metalloproteinase 8 in periodontitis patients: A randomized controlled clinical trial Journal of Advanced Periodontology and Implant Dentistry matrix metalloproteinase 8 periodontitis propolis randomized controlled trial saliva |
| title | Effect of propolis extract on clinical parameters and salivary level of matrix metalloproteinase 8 in periodontitis patients: A randomized controlled clinical trial |
| title_full | Effect of propolis extract on clinical parameters and salivary level of matrix metalloproteinase 8 in periodontitis patients: A randomized controlled clinical trial |
| title_fullStr | Effect of propolis extract on clinical parameters and salivary level of matrix metalloproteinase 8 in periodontitis patients: A randomized controlled clinical trial |
| title_full_unstemmed | Effect of propolis extract on clinical parameters and salivary level of matrix metalloproteinase 8 in periodontitis patients: A randomized controlled clinical trial |
| title_short | Effect of propolis extract on clinical parameters and salivary level of matrix metalloproteinase 8 in periodontitis patients: A randomized controlled clinical trial |
| title_sort | effect of propolis extract on clinical parameters and salivary level of matrix metalloproteinase 8 in periodontitis patients a randomized controlled clinical trial |
| topic | matrix metalloproteinase 8 periodontitis propolis randomized controlled trial saliva |
| url | https://japid.tbzmed.ac.ir/PDF/japid-13-84.pdf |
| work_keys_str_mv | AT reyhaneheghbalizarch effectofpropolisextractonclinicalparametersandsalivarylevelofmatrixmetalloproteinase8inperiodontitispatientsarandomizedcontrolledclinicaltrial AT mitraaskari effectofpropolisextractonclinicalparametersandsalivarylevelofmatrixmetalloproteinase8inperiodontitispatientsarandomizedcontrolledclinicaltrial AT hamidrezaboostani effectofpropolisextractonclinicalparametersandsalivarylevelofmatrixmetalloproteinase8inperiodontitispatientsarandomizedcontrolledclinicaltrial AT irajmirzaiidizgah effectofpropolisextractonclinicalparametersandsalivarylevelofmatrixmetalloproteinase8inperiodontitispatientsarandomizedcontrolledclinicaltrial |