Quantile normalization of single-cell RNA-seq read counts without unique molecular identifiers

Quantile normalization of single-cell RNA-seq read counts without unique molecular identifiers

Abstract Single-cell RNA-seq (scRNA-seq) profiles gene expression of individual cells. Unique molecular identifiers (UMIs) remove duplicates in read counts resulting from polymerase chain reaction, a major source of noise. For scRNA-seq data lacking UMIs, we propose quasi-UMIs: quantile normalizatio...

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Bibliographic Details
Main Authors: F. William Townes, Rafael A. Irizarry
Format: Article
Language:English
Published: BMC 2020-07-01
Series:Genome Biology
Subjects:
Gene expression
Single cell
RNA-seq
Normalization
Quasi-UMI
Online Access:http://link.springer.com/article/10.1186/s13059-020-02078-0

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