Expression of Zeb1 in the differentiation of mouse embryonic stem cell
Embryonic stem cells (ESCs) differentiation is a process of replication and refinement, and the directional lineage differentiation of ESCs involves the epithelial-mesenchymal transition (EMT)- mesenchymal-epithelial transition (MET) process. A previous study revealed that Zinc finger E-box-binding...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2022-05-01
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| Series: | Open Life Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/biol-2022-0042 |
| _version_ | 1797998132928184320 |
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| author | Chen Ting Pan Peng Wei Wei Zhang Yanmin Cui Guanghui Yu Zhendong Guo Xin |
| author_facet | Chen Ting Pan Peng Wei Wei Zhang Yanmin Cui Guanghui Yu Zhendong Guo Xin |
| author_sort | Chen Ting |
| collection | DOAJ |
| description | Embryonic stem cells (ESCs) differentiation is a process of replication and refinement, and the directional lineage differentiation of ESCs involves the epithelial-mesenchymal transition (EMT)- mesenchymal-epithelial transition (MET) process. A previous study revealed that Zinc finger E-box-binding homeobox 1 (Zeb1) plays a vital role in EMT, which could repress E-cadherin promoter and induce an EMT in cells. To verify the expression of Zeb1 and its correlation with Lin28a in mouse ESCs differentiation, we performed qRT-PCR and western blots to detect the expression of Lin28a mRNA and protein after Zeb1 knockdown. The expression of Zeb1 decreased over time of mouse ESCs differentiation but significantly increased in mouse embryonal carcinoma cells. After knockdown of Zeb1, Lin28a and Vimentin expression were decreased, while E-cadherin expression increased both in mouse ESCs, EBs, GC1, and P19 cells. We found that Zeb1 promoted the invasive ability of mouse embryonal carcinoma cells. These results revealed that expression of Zeb1 decreased during the differentiation of ESCs, and Lin28a and EMT processes can be regulated by Zeb1, which need to be verified in the future studies. |
| first_indexed | 2024-04-11T10:42:30Z |
| format | Article |
| id | doaj.art-c5ef7fd282874af3bed52ac3265c287e |
| institution | Directory Open Access Journal |
| issn | 2391-5412 |
| language | English |
| last_indexed | 2024-04-11T10:42:30Z |
| publishDate | 2022-05-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Life Sciences |
| spelling | doaj.art-c5ef7fd282874af3bed52ac3265c287e2022-12-22T04:29:08ZengDe GruyterOpen Life Sciences2391-54122022-05-0117145546210.1515/biol-2022-0042Expression of Zeb1 in the differentiation of mouse embryonic stem cellChen Ting0Pan Peng1Wei Wei2Zhang Yanmin3Cui Guanghui4Yu Zhendong5Guo Xin6Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, 1120 Lianhua Road, Shenzhen, Guangdong 518036, ChinaGuangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, 1120 Lianhua Road, Shenzhen, Guangdong 518036, ChinaDepartment of Blood Vessel Surgical Treatment Area, Changchun Provincial People’s Hospital, 1183 Industrial and Agricultural Road, Changchun, 130021, ChinaGuangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, 1120 Lianhua Road, Shenzhen, Guangdong 518036, ChinaGuangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, 1120 Lianhua Road, Shenzhen, Guangdong 518036, ChinaGuangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, 1120 Lianhua Road, Shenzhen, Guangdong 518036, ChinaGuangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, 1120 Lianhua Road, Shenzhen, Guangdong 518036, ChinaEmbryonic stem cells (ESCs) differentiation is a process of replication and refinement, and the directional lineage differentiation of ESCs involves the epithelial-mesenchymal transition (EMT)- mesenchymal-epithelial transition (MET) process. A previous study revealed that Zinc finger E-box-binding homeobox 1 (Zeb1) plays a vital role in EMT, which could repress E-cadherin promoter and induce an EMT in cells. To verify the expression of Zeb1 and its correlation with Lin28a in mouse ESCs differentiation, we performed qRT-PCR and western blots to detect the expression of Lin28a mRNA and protein after Zeb1 knockdown. The expression of Zeb1 decreased over time of mouse ESCs differentiation but significantly increased in mouse embryonal carcinoma cells. After knockdown of Zeb1, Lin28a and Vimentin expression were decreased, while E-cadherin expression increased both in mouse ESCs, EBs, GC1, and P19 cells. We found that Zeb1 promoted the invasive ability of mouse embryonal carcinoma cells. These results revealed that expression of Zeb1 decreased during the differentiation of ESCs, and Lin28a and EMT processes can be regulated by Zeb1, which need to be verified in the future studies.https://doi.org/10.1515/biol-2022-0042zeb1lin28aembryonic stem cellsmouse embryonal carcinoma cells |
| spellingShingle | Chen Ting Pan Peng Wei Wei Zhang Yanmin Cui Guanghui Yu Zhendong Guo Xin Expression of Zeb1 in the differentiation of mouse embryonic stem cell Open Life Sciences zeb1 lin28a embryonic stem cells mouse embryonal carcinoma cells |
| title | Expression of Zeb1 in the differentiation of mouse embryonic stem cell |
| title_full | Expression of Zeb1 in the differentiation of mouse embryonic stem cell |
| title_fullStr | Expression of Zeb1 in the differentiation of mouse embryonic stem cell |
| title_full_unstemmed | Expression of Zeb1 in the differentiation of mouse embryonic stem cell |
| title_short | Expression of Zeb1 in the differentiation of mouse embryonic stem cell |
| title_sort | expression of zeb1 in the differentiation of mouse embryonic stem cell |
| topic | zeb1 lin28a embryonic stem cells mouse embryonal carcinoma cells |
| url | https://doi.org/10.1515/biol-2022-0042 |
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