Analytical Studies of Antimicrobial Peptides as Diagnostic Biomarkers for the Detection of Bacterial and Viral Pneumonia
Pneumonia remains one of the leading causes of infectious mortality and significant economic losses among our growing population. The lack of specific biomarkers for correct and timely diagnosis to detect patients’ status is a bane towards initiating a proper treatment plan for the disease; thus, cu...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/9/7/305 |
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| author | Olalekan Olanrewaju Bakare Arun Gokul Marshall Keyster |
| author_facet | Olalekan Olanrewaju Bakare Arun Gokul Marshall Keyster |
| author_sort | Olalekan Olanrewaju Bakare |
| collection | DOAJ |
| description | Pneumonia remains one of the leading causes of infectious mortality and significant economic losses among our growing population. The lack of specific biomarkers for correct and timely diagnosis to detect patients’ status is a bane towards initiating a proper treatment plan for the disease; thus, current biomarkers cannot distinguish between pneumonia and other associated conditions such as atherosclerotic plaques and human immunodeficiency virus (HIV). Antimicrobial peptides (AMPs) are potential candidates for detecting numerous illnesses due to their compensatory roles as theranostic molecules. This research sought to generate specific data for parental AMPs to identify viral and bacterial pneumonia pathogens using in silico technology. The parental antimicrobial peptides (AMPs) used in this work were AMPs discovered in our previous in silico analyses using the HMMER algorithm, which were used to generate derivative (mutated) AMPs that would bind with greater affinity, in order to detect the bacterial and viral receptors using an in silico site-directed mutagenesis approach. These AMPs’ 3D structures were subsequently predicted and docked against receptor proteins. The result shows putative AMPs with the potential capacity to detect pneumonia caused by these pathogens through their binding precision with high sensitivity, accuracy, and specificity for possible use in point-of-care diagnosis. These peptides’ tendency to detect receptor proteins of viral and bacterial pneumonia with precision justifies their use for differential diagnostics, in an attempt to reduce the problems of indiscriminate overuse, toxicity due to the wrong prescription, bacterial resistance, and the scarcity and high cost of existing pneumonia antibiotics. |
| first_indexed | 2024-03-09T10:22:35Z |
| format | Article |
| id | doaj.art-c5f187cd0b0a460583b8d58e7216614e |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-09T10:22:35Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-c5f187cd0b0a460583b8d58e7216614e2023-12-01T21:53:54ZengMDPI AGBioengineering2306-53542022-07-019730510.3390/bioengineering9070305Analytical Studies of Antimicrobial Peptides as Diagnostic Biomarkers for the Detection of Bacterial and Viral PneumoniaOlalekan Olanrewaju Bakare0Arun Gokul1Marshall Keyster2Environmental Biotechnology Laboratory (EBL), Department of Biotechnology, University of the Western Cape, Cape Town 7535, South AfricaEnvironmental Biotechnology Laboratory (EBL), Department of Biotechnology, University of the Western Cape, Cape Town 7535, South AfricaEnvironmental Biotechnology Laboratory (EBL), Department of Biotechnology, University of the Western Cape, Cape Town 7535, South AfricaPneumonia remains one of the leading causes of infectious mortality and significant economic losses among our growing population. The lack of specific biomarkers for correct and timely diagnosis to detect patients’ status is a bane towards initiating a proper treatment plan for the disease; thus, current biomarkers cannot distinguish between pneumonia and other associated conditions such as atherosclerotic plaques and human immunodeficiency virus (HIV). Antimicrobial peptides (AMPs) are potential candidates for detecting numerous illnesses due to their compensatory roles as theranostic molecules. This research sought to generate specific data for parental AMPs to identify viral and bacterial pneumonia pathogens using in silico technology. The parental antimicrobial peptides (AMPs) used in this work were AMPs discovered in our previous in silico analyses using the HMMER algorithm, which were used to generate derivative (mutated) AMPs that would bind with greater affinity, in order to detect the bacterial and viral receptors using an in silico site-directed mutagenesis approach. These AMPs’ 3D structures were subsequently predicted and docked against receptor proteins. The result shows putative AMPs with the potential capacity to detect pneumonia caused by these pathogens through their binding precision with high sensitivity, accuracy, and specificity for possible use in point-of-care diagnosis. These peptides’ tendency to detect receptor proteins of viral and bacterial pneumonia with precision justifies their use for differential diagnostics, in an attempt to reduce the problems of indiscriminate overuse, toxicity due to the wrong prescription, bacterial resistance, and the scarcity and high cost of existing pneumonia antibiotics.https://www.mdpi.com/2306-5354/9/7/305antimicrobial peptidesbacteriavirusesdatabasesmachine learning tooldiagnostics |
| spellingShingle | Olalekan Olanrewaju Bakare Arun Gokul Marshall Keyster Analytical Studies of Antimicrobial Peptides as Diagnostic Biomarkers for the Detection of Bacterial and Viral Pneumonia Bioengineering antimicrobial peptides bacteria viruses databases machine learning tool diagnostics |
| title | Analytical Studies of Antimicrobial Peptides as Diagnostic Biomarkers for the Detection of Bacterial and Viral Pneumonia |
| title_full | Analytical Studies of Antimicrobial Peptides as Diagnostic Biomarkers for the Detection of Bacterial and Viral Pneumonia |
| title_fullStr | Analytical Studies of Antimicrobial Peptides as Diagnostic Biomarkers for the Detection of Bacterial and Viral Pneumonia |
| title_full_unstemmed | Analytical Studies of Antimicrobial Peptides as Diagnostic Biomarkers for the Detection of Bacterial and Viral Pneumonia |
| title_short | Analytical Studies of Antimicrobial Peptides as Diagnostic Biomarkers for the Detection of Bacterial and Viral Pneumonia |
| title_sort | analytical studies of antimicrobial peptides as diagnostic biomarkers for the detection of bacterial and viral pneumonia |
| topic | antimicrobial peptides bacteria viruses databases machine learning tool diagnostics |
| url | https://www.mdpi.com/2306-5354/9/7/305 |
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