Systems parasitology: effects of Fasciola hepatica on the neurochemical profile in the rat brain
Abstract We characterize the integrated response of a rat host to the liver fluke Fasciola hepatica using a combination of 1H nuclear magnetic resonance spectroscopic profiles (liver, kidney, intestine, brain, spleen, plasma, urine, feces) and multiplex cytokine markers of systemic inflammation. Mul...
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| Format: | Article |
| Language: | English |
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Springer Nature
2010-07-01
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| Series: | Molecular Systems Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/msb.2010.49 |
| _version_ | 1826990468163960832 |
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| author | Jasmina Saric Jia V Li Jürg Utzinger Yulan Wang Jennifer Keiser Stephan Dirnhofer Olaf Beckonert Mansour T A Sharabiani Judith M Fonville Jeremy K Nicholson Elaine Holmes |
| author_facet | Jasmina Saric Jia V Li Jürg Utzinger Yulan Wang Jennifer Keiser Stephan Dirnhofer Olaf Beckonert Mansour T A Sharabiani Judith M Fonville Jeremy K Nicholson Elaine Holmes |
| author_sort | Jasmina Saric |
| collection | DOAJ |
| description | Abstract We characterize the integrated response of a rat host to the liver fluke Fasciola hepatica using a combination of 1H nuclear magnetic resonance spectroscopic profiles (liver, kidney, intestine, brain, spleen, plasma, urine, feces) and multiplex cytokine markers of systemic inflammation. Multivariate mathematical models were built to describe the main features of the infection at the systems level. In addition to the expected modulation of hepatic choline and energy metabolism, we found significant perturbations of the nucleotide balance in the brain, together with increased plasma IL‐13, suggesting a shift toward modulation of immune reactions to minimize inflammatory damage, which may favor the co‐existence of the parasite in the host. Subsequent analysis of brain extracts from other trematode infection models (i.e. Schistosoma mansoni, and Echinostoma caproni) did not elicit a change in neural nucleotide levels, indicating that the neural effects of F. hepatica infection are specific. We propose that the topographically extended response to invasion of the host as characterized by the modulated global metabolic phenotype is stratified across several bio‐organizational levels and reflects the direct manipulation of host–nucleotide balance. |
| first_indexed | 2024-03-07T16:38:44Z |
| format | Article |
| id | doaj.art-c5f2b867c9e94694b1d010bb41d92d71 |
| institution | Directory Open Access Journal |
| issn | 1744-4292 |
| language | English |
| last_indexed | 2025-02-18T08:21:03Z |
| publishDate | 2010-07-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | Molecular Systems Biology |
| spelling | doaj.art-c5f2b867c9e94694b1d010bb41d92d712024-11-03T12:53:46ZengSpringer NatureMolecular Systems Biology1744-42922010-07-016111010.1038/msb.2010.49Systems parasitology: effects of Fasciola hepatica on the neurochemical profile in the rat brainJasmina Saric0Jia V Li1Jürg Utzinger2Yulan Wang3Jennifer Keiser4Stephan Dirnhofer5Olaf Beckonert6Mansour T A Sharabiani7Judith M Fonville8Jeremy K Nicholson9Elaine Holmes10Biomolecular Medicine, Department of Surgery and Cancer, Faculty of Medicine, Imperial College LondonBiomolecular Medicine, Department of Surgery and Cancer, Faculty of Medicine, Imperial College LondonDepartment of Epidemiology and Public Health, Swiss Tropical and Public Health InstituteState Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Center for Magnetic Resonance, Wuhan Institute of Physics and Mathematics, Chinese Academy of SciencesUniversity of BaselInstitute of Pathology, University Hospital BaselBiomolecular Medicine, Department of Surgery and Cancer, Faculty of Medicine, Imperial College LondonDepartment of Epidemiology and Biostatistics, School of Public Health, Faculty of Medicine, Imperial College LondonBiomolecular Medicine, Department of Surgery and Cancer, Faculty of Medicine, Imperial College LondonBiomolecular Medicine, Department of Surgery and Cancer, Faculty of Medicine, Imperial College LondonBiomolecular Medicine, Department of Surgery and Cancer, Faculty of Medicine, Imperial College LondonAbstract We characterize the integrated response of a rat host to the liver fluke Fasciola hepatica using a combination of 1H nuclear magnetic resonance spectroscopic profiles (liver, kidney, intestine, brain, spleen, plasma, urine, feces) and multiplex cytokine markers of systemic inflammation. Multivariate mathematical models were built to describe the main features of the infection at the systems level. In addition to the expected modulation of hepatic choline and energy metabolism, we found significant perturbations of the nucleotide balance in the brain, together with increased plasma IL‐13, suggesting a shift toward modulation of immune reactions to minimize inflammatory damage, which may favor the co‐existence of the parasite in the host. Subsequent analysis of brain extracts from other trematode infection models (i.e. Schistosoma mansoni, and Echinostoma caproni) did not elicit a change in neural nucleotide levels, indicating that the neural effects of F. hepatica infection are specific. We propose that the topographically extended response to invasion of the host as characterized by the modulated global metabolic phenotype is stratified across several bio‐organizational levels and reflects the direct manipulation of host–nucleotide balance.https://doi.org/10.1038/msb.2010.49brainFasciola hepaticaimmunologymetabolic profilingnuclear magnetic resonance spectroscopy |
| spellingShingle | Jasmina Saric Jia V Li Jürg Utzinger Yulan Wang Jennifer Keiser Stephan Dirnhofer Olaf Beckonert Mansour T A Sharabiani Judith M Fonville Jeremy K Nicholson Elaine Holmes Systems parasitology: effects of Fasciola hepatica on the neurochemical profile in the rat brain Molecular Systems Biology brain Fasciola hepatica immunology metabolic profiling nuclear magnetic resonance spectroscopy |
| title | Systems parasitology: effects of Fasciola hepatica on the neurochemical profile in the rat brain |
| title_full | Systems parasitology: effects of Fasciola hepatica on the neurochemical profile in the rat brain |
| title_fullStr | Systems parasitology: effects of Fasciola hepatica on the neurochemical profile in the rat brain |
| title_full_unstemmed | Systems parasitology: effects of Fasciola hepatica on the neurochemical profile in the rat brain |
| title_short | Systems parasitology: effects of Fasciola hepatica on the neurochemical profile in the rat brain |
| title_sort | systems parasitology effects of fasciola hepatica on the neurochemical profile in the rat brain |
| topic | brain Fasciola hepatica immunology metabolic profiling nuclear magnetic resonance spectroscopy |
| url | https://doi.org/10.1038/msb.2010.49 |
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