Presence–absence polymorphisms of single-copy genes in the stony coral Acropora digitifera
Abstract Background Despite the importance of characterizing genetic variation among coral individuals for understanding phenotypic variation, the correlation between coral genomic diversity and phenotypic expression is still poorly understood. Results In this study, we detected a high frequency of...
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BMC
2020-02-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-020-6566-4 |
| _version_ | 1818417459166707712 |
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| author | Shiho Takahashi-Kariyazono Kazuhiko Sakai Yohey Terai |
| author_facet | Shiho Takahashi-Kariyazono Kazuhiko Sakai Yohey Terai |
| author_sort | Shiho Takahashi-Kariyazono |
| collection | DOAJ |
| description | Abstract Background Despite the importance of characterizing genetic variation among coral individuals for understanding phenotypic variation, the correlation between coral genomic diversity and phenotypic expression is still poorly understood. Results In this study, we detected a high frequency of genes showing presence–absence polymorphisms (PAPs) for single-copy genes in Acropora digitifera. Among 10,455 single-copy genes, 516 (5%) exhibited PAPs, including 32 transposable element (TE)-related genes. Five hundred sixteen genes exhibited a homozygous absence in one (102) or more than one (414) individuals (n = 33), indicating that most of the absent alleles were not rare variants. Among genes showing PAPs (PAP genes), roughly half were expressed in adults and/or larvae, and the PAP status was associated with differential expression among individuals. Although 85% of PAP genes were uncharacterized or had ambiguous annotations, 70% of these genes were specifically distributed in cnidarian lineages in eumetazoa, suggesting that these genes have functional roles related to traits related to cnidarians or the family Acroporidae or the genus Acropora. Indeed, four of these genes encoded toxins that are usually components of venom in cnidarian-specific cnidocytes. At least 17% of A. digitifera PAP genes were also PAPs in A. tenuis, the basal lineage in the genus Acropora, indicating that PAPs were shared among species in Acropora. Conclusions Expression differences caused by a high frequency of PAP genes may be a novel genomic feature in the genus Acropora; these findings will contribute to improve our understanding of correlation between genetic and phenotypic variation in corals. |
| first_indexed | 2024-12-14T12:07:07Z |
| format | Article |
| id | doaj.art-c5f4bca098a643de80329318e11a5cfb |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-14T12:07:07Z |
| publishDate | 2020-02-01 |
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| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-c5f4bca098a643de80329318e11a5cfb2022-12-21T23:01:51ZengBMCBMC Genomics1471-21642020-02-0121111310.1186/s12864-020-6566-4Presence–absence polymorphisms of single-copy genes in the stony coral Acropora digitiferaShiho Takahashi-Kariyazono0Kazuhiko Sakai1Yohey Terai2Department of Evolutionary Studies of Biosystems, SOKENDAI (The Graduate University for Advanced Studies)Sesoko Station, Tropical Biosphere Research Center, University of the RyukyusDepartment of Evolutionary Studies of Biosystems, SOKENDAI (The Graduate University for Advanced Studies)Abstract Background Despite the importance of characterizing genetic variation among coral individuals for understanding phenotypic variation, the correlation between coral genomic diversity and phenotypic expression is still poorly understood. Results In this study, we detected a high frequency of genes showing presence–absence polymorphisms (PAPs) for single-copy genes in Acropora digitifera. Among 10,455 single-copy genes, 516 (5%) exhibited PAPs, including 32 transposable element (TE)-related genes. Five hundred sixteen genes exhibited a homozygous absence in one (102) or more than one (414) individuals (n = 33), indicating that most of the absent alleles were not rare variants. Among genes showing PAPs (PAP genes), roughly half were expressed in adults and/or larvae, and the PAP status was associated with differential expression among individuals. Although 85% of PAP genes were uncharacterized or had ambiguous annotations, 70% of these genes were specifically distributed in cnidarian lineages in eumetazoa, suggesting that these genes have functional roles related to traits related to cnidarians or the family Acroporidae or the genus Acropora. Indeed, four of these genes encoded toxins that are usually components of venom in cnidarian-specific cnidocytes. At least 17% of A. digitifera PAP genes were also PAPs in A. tenuis, the basal lineage in the genus Acropora, indicating that PAPs were shared among species in Acropora. Conclusions Expression differences caused by a high frequency of PAP genes may be a novel genomic feature in the genus Acropora; these findings will contribute to improve our understanding of correlation between genetic and phenotypic variation in corals.https://doi.org/10.1186/s12864-020-6566-4Structural variationExpression differenceGenome diversity |
| spellingShingle | Shiho Takahashi-Kariyazono Kazuhiko Sakai Yohey Terai Presence–absence polymorphisms of single-copy genes in the stony coral Acropora digitifera BMC Genomics Structural variation Expression difference Genome diversity |
| title | Presence–absence polymorphisms of single-copy genes in the stony coral Acropora digitifera |
| title_full | Presence–absence polymorphisms of single-copy genes in the stony coral Acropora digitifera |
| title_fullStr | Presence–absence polymorphisms of single-copy genes in the stony coral Acropora digitifera |
| title_full_unstemmed | Presence–absence polymorphisms of single-copy genes in the stony coral Acropora digitifera |
| title_short | Presence–absence polymorphisms of single-copy genes in the stony coral Acropora digitifera |
| title_sort | presence absence polymorphisms of single copy genes in the stony coral acropora digitifera |
| topic | Structural variation Expression difference Genome diversity |
| url | https://doi.org/10.1186/s12864-020-6566-4 |
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