The Use of Juçara (Euterpe edulis Mart.) Supplementation for Suppression of NF-κB Pathway in the Hypothalamus after High-Fat Diet in Wistar Rats
Obesity is associated with modern diets that are rich in saturated fatty acids. These dietary patterns are linked to low-grade proinflammatory mechanisms, such as the toll-like receptor 4/nuclear factor kappa-B (NF-κB) pathway rapidly activated through high-fat diets. Juçara is...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2018-07-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1420-3049/23/7/1814 |
| _version_ | 1811269587171278848 |
|---|---|
| author | Aline Boveto Santamarina Giovana Jamar Laís Vales Mennitti Veridiana Vera de Rosso Helena Cassia Cesar Lila Missae Oyama Luciana Pellegrini Pisani |
| author_facet | Aline Boveto Santamarina Giovana Jamar Laís Vales Mennitti Veridiana Vera de Rosso Helena Cassia Cesar Lila Missae Oyama Luciana Pellegrini Pisani |
| author_sort | Aline Boveto Santamarina |
| collection | DOAJ |
| description | Obesity is associated with modern diets that are rich in saturated fatty acids. These dietary patterns are linked to low-grade proinflammatory mechanisms, such as the toll-like receptor 4/nuclear factor kappa-B (NF-κB) pathway rapidly activated through high-fat diets. Juçara is a berry rich in anthocyanins and unsaturated fatty acids, which prevents obesity and associated comorbidities. We evaluated the effect of different doses of freeze-dried juçara pulp on NF-κB pathway after the consumption of short-term high-fat diet. Male Wistar rats with ad libitum access to food and water were divided into four groups: Control diet (C), high-fat diet (HFC), high-fat diet with 0.25% juçara (HFJ 0.25%), and high-fat diet with 0.5% juçara (HFJ 0.5%). Energy intake and body weight gain were increased in HFC and HFJ 0.5% groups compared to C group. The hypothalamus weight reduced in the HFC group compared to C and HFJ 0.25% groups. Cytokines, MYD88, TRAF6, and pNF-κBp50 levels in the hypothalamus, serum triacylglycerol, LDL-cholesterol (LDL-C), and free fatty acid levels were improved in the HFJ 0.25% group. In summary, the HFJ 0.25% group had better protective effects than those in the HFJ 0.5%. Therefore, 0.25% juçara can be used to protect against central inflammation through the high-fat diet-induced NF-κB pathway. |
| first_indexed | 2024-04-12T21:44:45Z |
| format | Article |
| id | doaj.art-c5f5a0e4f06f4bf0a84c55d9cffadc8b |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-12T21:44:45Z |
| publishDate | 2018-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-c5f5a0e4f06f4bf0a84c55d9cffadc8b2022-12-22T03:15:40ZengMDPI AGMolecules1420-30492018-07-01237181410.3390/molecules23071814molecules23071814The Use of Juçara (Euterpe edulis Mart.) Supplementation for Suppression of NF-κB Pathway in the Hypothalamus after High-Fat Diet in Wistar RatsAline Boveto Santamarina0Giovana Jamar1Laís Vales Mennitti2Veridiana Vera de Rosso3Helena Cassia Cesar4Lila Missae Oyama5Luciana Pellegrini Pisani6Programa de Pós-Graduação Interdisciplinar em Ciências da Saúde, Universidade Federal de São Paulo, São Paulo 11015-020, BrazilPrograma de Pós-Graduação Interdisciplinar em Ciências da Saúde, Universidade Federal de São Paulo, São Paulo 11015-020, BrazilPrograma de Pós-Graduação Interdisciplinar em Ciências da Saúde, Universidade Federal de São Paulo, São Paulo 11015-020, BrazilDepartamento de Biociências, Universidade Federal de São Paulo, São Paulo 11015-020, BrazilPrograma de Pós-Graduação Interdisciplinar em Ciências da Saúde, Universidade Federal de São Paulo, São Paulo 11015-020, BrazilDepartamento de Fisiologia, Universidade Federal de São Paulo, São Paulo 04023-062, BrazilDepartamento de Biociências, Universidade Federal de São Paulo, São Paulo 11015-020, BrazilObesity is associated with modern diets that are rich in saturated fatty acids. These dietary patterns are linked to low-grade proinflammatory mechanisms, such as the toll-like receptor 4/nuclear factor kappa-B (NF-κB) pathway rapidly activated through high-fat diets. Juçara is a berry rich in anthocyanins and unsaturated fatty acids, which prevents obesity and associated comorbidities. We evaluated the effect of different doses of freeze-dried juçara pulp on NF-κB pathway after the consumption of short-term high-fat diet. Male Wistar rats with ad libitum access to food and water were divided into four groups: Control diet (C), high-fat diet (HFC), high-fat diet with 0.25% juçara (HFJ 0.25%), and high-fat diet with 0.5% juçara (HFJ 0.5%). Energy intake and body weight gain were increased in HFC and HFJ 0.5% groups compared to C group. The hypothalamus weight reduced in the HFC group compared to C and HFJ 0.25% groups. Cytokines, MYD88, TRAF6, and pNF-κBp50 levels in the hypothalamus, serum triacylglycerol, LDL-cholesterol (LDL-C), and free fatty acid levels were improved in the HFJ 0.25% group. In summary, the HFJ 0.25% group had better protective effects than those in the HFJ 0.5%. Therefore, 0.25% juçara can be used to protect against central inflammation through the high-fat diet-induced NF-κB pathway.http://www.mdpi.com/1420-3049/23/7/1814juçarainflammationhypothalamusshort-term high-fat dietNF-κB pathway |
| spellingShingle | Aline Boveto Santamarina Giovana Jamar Laís Vales Mennitti Veridiana Vera de Rosso Helena Cassia Cesar Lila Missae Oyama Luciana Pellegrini Pisani The Use of Juçara (Euterpe edulis Mart.) Supplementation for Suppression of NF-κB Pathway in the Hypothalamus after High-Fat Diet in Wistar Rats Molecules juçara inflammation hypothalamus short-term high-fat diet NF-κB pathway |
| title | The Use of Juçara (Euterpe edulis Mart.) Supplementation for Suppression of NF-κB Pathway in the Hypothalamus after High-Fat Diet in Wistar Rats |
| title_full | The Use of Juçara (Euterpe edulis Mart.) Supplementation for Suppression of NF-κB Pathway in the Hypothalamus after High-Fat Diet in Wistar Rats |
| title_fullStr | The Use of Juçara (Euterpe edulis Mart.) Supplementation for Suppression of NF-κB Pathway in the Hypothalamus after High-Fat Diet in Wistar Rats |
| title_full_unstemmed | The Use of Juçara (Euterpe edulis Mart.) Supplementation for Suppression of NF-κB Pathway in the Hypothalamus after High-Fat Diet in Wistar Rats |
| title_short | The Use of Juçara (Euterpe edulis Mart.) Supplementation for Suppression of NF-κB Pathway in the Hypothalamus after High-Fat Diet in Wistar Rats |
| title_sort | use of jucara euterpe edulis mart supplementation for suppression of nf κb pathway in the hypothalamus after high fat diet in wistar rats |
| topic | juçara inflammation hypothalamus short-term high-fat diet NF-κB pathway |
| url | http://www.mdpi.com/1420-3049/23/7/1814 |
| work_keys_str_mv | AT alinebovetosantamarina theuseofjucaraeuterpeedulismartsupplementationforsuppressionofnfkbpathwayinthehypothalamusafterhighfatdietinwistarrats AT giovanajamar theuseofjucaraeuterpeedulismartsupplementationforsuppressionofnfkbpathwayinthehypothalamusafterhighfatdietinwistarrats AT laisvalesmennitti theuseofjucaraeuterpeedulismartsupplementationforsuppressionofnfkbpathwayinthehypothalamusafterhighfatdietinwistarrats AT veridianaveraderosso theuseofjucaraeuterpeedulismartsupplementationforsuppressionofnfkbpathwayinthehypothalamusafterhighfatdietinwistarrats AT helenacassiacesar theuseofjucaraeuterpeedulismartsupplementationforsuppressionofnfkbpathwayinthehypothalamusafterhighfatdietinwistarrats AT lilamissaeoyama theuseofjucaraeuterpeedulismartsupplementationforsuppressionofnfkbpathwayinthehypothalamusafterhighfatdietinwistarrats AT lucianapellegrinipisani theuseofjucaraeuterpeedulismartsupplementationforsuppressionofnfkbpathwayinthehypothalamusafterhighfatdietinwistarrats AT alinebovetosantamarina useofjucaraeuterpeedulismartsupplementationforsuppressionofnfkbpathwayinthehypothalamusafterhighfatdietinwistarrats AT giovanajamar useofjucaraeuterpeedulismartsupplementationforsuppressionofnfkbpathwayinthehypothalamusafterhighfatdietinwistarrats AT laisvalesmennitti useofjucaraeuterpeedulismartsupplementationforsuppressionofnfkbpathwayinthehypothalamusafterhighfatdietinwistarrats AT veridianaveraderosso useofjucaraeuterpeedulismartsupplementationforsuppressionofnfkbpathwayinthehypothalamusafterhighfatdietinwistarrats AT helenacassiacesar useofjucaraeuterpeedulismartsupplementationforsuppressionofnfkbpathwayinthehypothalamusafterhighfatdietinwistarrats AT lilamissaeoyama useofjucaraeuterpeedulismartsupplementationforsuppressionofnfkbpathwayinthehypothalamusafterhighfatdietinwistarrats AT lucianapellegrinipisani useofjucaraeuterpeedulismartsupplementationforsuppressionofnfkbpathwayinthehypothalamusafterhighfatdietinwistarrats |