Soil Bacterial Diversity Responds to Long-Term Establishment of Perennial Legumes in Warm-Season Grassland at Two Soil Depths
The introduction of rhizoma peanut (RP <i>Arachis glabrata</i> Benth) into bahiagrass (<i>Paspalum notatum</i> Flüggé) may require time to develop stable plant–soil microbe interactions as the microbial legacy of the previous plant community may be long-lasting. A previous st...
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MDPI AG
2023-12-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/12/3002 |
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| author | Adesuwa Sylvia Erhunmwunse Victor Alonso Guerra Jung-Chen Liu Cheryl L. Mackowiak Ann Rachel Soffes Blount José Carlos Batista Dubeux Hui-Ling Liao |
| author_facet | Adesuwa Sylvia Erhunmwunse Victor Alonso Guerra Jung-Chen Liu Cheryl L. Mackowiak Ann Rachel Soffes Blount José Carlos Batista Dubeux Hui-Ling Liao |
| author_sort | Adesuwa Sylvia Erhunmwunse |
| collection | DOAJ |
| description | The introduction of rhizoma peanut (RP <i>Arachis glabrata</i> Benth) into bahiagrass (<i>Paspalum notatum</i> Flüggé) may require time to develop stable plant–soil microbe interactions as the microbial legacy of the previous plant community may be long-lasting. A previous study showed that <2 years of introducing rhizoma peanut into bahiagrass pastures minimally affected soil bacterial diversity and community composition. In this study, we compared the effects of the long-term inclusion of rhizoma peanut (>8 years) into bahiagrass on soil bacterial diversity and community composition against their monocultures at 0 to 15 and 15 to 30 cm soil depths using next-generation sequencing to target bacterial 16S V3–V4 regions. We observed that a well-established RP–bahiagrass mixed stand led to a 36% increase in bacterial alpha diversity compared to the bahiagrass monoculture. There was a shift from a soil bacterial community dominated by Proteobacteria (~26%) reported in other bahiagrass and rhizoma peanut studies to a soil bacterial community dominated by Firmicutes (39%) in our study. The relative abundance of the bacterial genus <i>Crossiella</i>, known for its antimicrobial traits, was enhanced in the presence of RP. Differences in soil bacterial diversity and community composition were substantial between 0 to 15 and 15 to 30 cm soil layers, with N<sub>2</sub>-fixing bacteria belonging to the phylum Proteobacteria concentrated in 0 to 15 cm. Introducing RP into bahiagrass pastures is a highly sustainable alternative to mineral N fertilizer inputs. Our results provide evidence that this system also promotes greater soil microbial diversity and is associated with unique taxa that require further study to better understand their contributions to healthy pastures. |
| first_indexed | 2024-03-08T20:31:17Z |
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| id | doaj.art-c5f8f6ebd1374c96bb82c1b3c0fb1747 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-08T20:31:17Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-c5f8f6ebd1374c96bb82c1b3c0fb17472023-12-22T14:26:21ZengMDPI AGMicroorganisms2076-26072023-12-011112300210.3390/microorganisms11123002Soil Bacterial Diversity Responds to Long-Term Establishment of Perennial Legumes in Warm-Season Grassland at Two Soil DepthsAdesuwa Sylvia Erhunmwunse0Victor Alonso Guerra1Jung-Chen Liu2Cheryl L. Mackowiak3Ann Rachel Soffes Blount4José Carlos Batista Dubeux5Hui-Ling Liao6North Florida Research and Education Center, University of Florida, 155 Research Road, Quincy, FL 32351, USANorth Florida Research and Education Center, University of Florida, 155 Research Road, Quincy, FL 32351, USANorth Florida Research and Education Center, University of Florida, 155 Research Road, Quincy, FL 32351, USANorth Florida Research and Education Center, University of Florida, 155 Research Road, Quincy, FL 32351, USANorth Florida Research and Education Center, University of Florida, 155 Research Road, Quincy, FL 32351, USANorth Florida Research and Education Center, University of Florida, 3925 Highway 71, Marianna, FL 32446, USANorth Florida Research and Education Center, University of Florida, 155 Research Road, Quincy, FL 32351, USAThe introduction of rhizoma peanut (RP <i>Arachis glabrata</i> Benth) into bahiagrass (<i>Paspalum notatum</i> Flüggé) may require time to develop stable plant–soil microbe interactions as the microbial legacy of the previous plant community may be long-lasting. A previous study showed that <2 years of introducing rhizoma peanut into bahiagrass pastures minimally affected soil bacterial diversity and community composition. In this study, we compared the effects of the long-term inclusion of rhizoma peanut (>8 years) into bahiagrass on soil bacterial diversity and community composition against their monocultures at 0 to 15 and 15 to 30 cm soil depths using next-generation sequencing to target bacterial 16S V3–V4 regions. We observed that a well-established RP–bahiagrass mixed stand led to a 36% increase in bacterial alpha diversity compared to the bahiagrass monoculture. There was a shift from a soil bacterial community dominated by Proteobacteria (~26%) reported in other bahiagrass and rhizoma peanut studies to a soil bacterial community dominated by Firmicutes (39%) in our study. The relative abundance of the bacterial genus <i>Crossiella</i>, known for its antimicrobial traits, was enhanced in the presence of RP. Differences in soil bacterial diversity and community composition were substantial between 0 to 15 and 15 to 30 cm soil layers, with N<sub>2</sub>-fixing bacteria belonging to the phylum Proteobacteria concentrated in 0 to 15 cm. Introducing RP into bahiagrass pastures is a highly sustainable alternative to mineral N fertilizer inputs. Our results provide evidence that this system also promotes greater soil microbial diversity and is associated with unique taxa that require further study to better understand their contributions to healthy pastures.https://www.mdpi.com/2076-2607/11/12/3002rhizoma peanutbahiagrassalpha diversitybeta diversityN fixing bacteria |
| spellingShingle | Adesuwa Sylvia Erhunmwunse Victor Alonso Guerra Jung-Chen Liu Cheryl L. Mackowiak Ann Rachel Soffes Blount José Carlos Batista Dubeux Hui-Ling Liao Soil Bacterial Diversity Responds to Long-Term Establishment of Perennial Legumes in Warm-Season Grassland at Two Soil Depths Microorganisms rhizoma peanut bahiagrass alpha diversity beta diversity N fixing bacteria |
| title | Soil Bacterial Diversity Responds to Long-Term Establishment of Perennial Legumes in Warm-Season Grassland at Two Soil Depths |
| title_full | Soil Bacterial Diversity Responds to Long-Term Establishment of Perennial Legumes in Warm-Season Grassland at Two Soil Depths |
| title_fullStr | Soil Bacterial Diversity Responds to Long-Term Establishment of Perennial Legumes in Warm-Season Grassland at Two Soil Depths |
| title_full_unstemmed | Soil Bacterial Diversity Responds to Long-Term Establishment of Perennial Legumes in Warm-Season Grassland at Two Soil Depths |
| title_short | Soil Bacterial Diversity Responds to Long-Term Establishment of Perennial Legumes in Warm-Season Grassland at Two Soil Depths |
| title_sort | soil bacterial diversity responds to long term establishment of perennial legumes in warm season grassland at two soil depths |
| topic | rhizoma peanut bahiagrass alpha diversity beta diversity N fixing bacteria |
| url | https://www.mdpi.com/2076-2607/11/12/3002 |
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