The Effects of Lactose Induction on a Plasmid-Free <i>E. coli</i> T7 Expression System

Recombinant production of pharmaceutical proteins like antigen binding fragments (Fabs) in the commonly-used production host <i>Escherichia coli</i> presents several challenges. The predominantly-used plasmid-based expression systems exhibit the drawback of either excessive plasmid ampli...

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Bibliographic Details
Main Authors: Johanna Hausjell, Regina Kutscha, Jeannine D. Gesson, Daniela Reinisch, Oliver Spadiut
Format: Article
Language:English
Published: MDPI AG 2020-01-01
Series:Bioengineering
Subjects:
Online Access:https://www.mdpi.com/2306-5354/7/1/8